山东海洋技术创新平台的发展与对策

为促进山东海洋技术创新和海洋产业发展,文章梳理山东海洋技术创新平台的发展现状,分析存在的问题并提出对策建议。研究结果表明：山东海洋技术创新平台体系建设日趋完善、种类和数量实现新突破并推动海洋产业高质量发展,但仍存在整体发展水平较低,地区和产业分布不均衡,省级、高端和新型平台不足以及企业主体创新力量作用较弱等问题;未来山...     

Formation and early development of tetraspores of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m-2 s-1 in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.     

Effects of salinity and nutrients on the growth and chlorophyll fluorescence of Caulerpa lentillifera

Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate(SGR) and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50,and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence( Fv / Fm) were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO 4-P. The photosynthetic capacity of photosystem II(PSII) was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO3-N concentrations were greater than 1.0 mmol/L and when PO4- P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera,these data contribute important information to ensure optimal results.     

藏北高原安多地区高山草甸土的母质成因及其成土模式

基于对藏北高原安多地区高山草甸士的粒度组成及理化性质的研究,探讨了该地区高山草甸土的母质成因及成土模式.分析结果表明,安多地区高山草甸土的粒度分布以20μm为界线呈双峰态分布.主峰峰值粒径在90 μm左右,较细峰呈宽阔细尾分布,峰值粒径在3～6 μm之间.高山草甸土的各粒级组成(＜2μm、2～20 μm、20 ～ 300 μm、300 ～2 000 μm)沿剖面变化很小.高山草甸土主要由极细砂组成(50～ 125 μm),平均粒径集中在60～90 μm,明显比黄土高原黄土、川西黄土、成都粘土的粗.高山草甸土粒度分布特征与土壤底部的薄层粗骨性残留古风化层相比存在显著差别,而与该区域河谷沉积、风尘沉积具有相似的特征.总之,各实验数据指示,安多地区高山草甸土具有土层厚、质地均匀、无砾石和层理不发育的特点,与基岩原地风化形成的高山草甸土存在明显差别,其成土母质来源于风尘沉积.该区高山草甸土成土过程符合风尘“加积型”土壤发育模式,而风尘沉积在高山草甸土的形成和发育过程中发挥了重要的作用.     

冻结季沱沱河源多年冻土区活动层土壤水分含量分析

活动层含水量是表征多年冻土区气候、水文和生态过程的关键参数。长期以来,由于受多年冻土区活动层水分实测样点数量稀少的限制,各类基于遥感反演、模式模拟乃至数据融合和同化等手段生产的土壤水分空间数据均存在着较大的误差。2020年10—11月在青藏高原腹地（沱沱河源区）测定了 1 072组活动层土壤含水量数据并进行分析,探讨了该时段该区域活动层土壤水分的空间差异,并与全球陆面数据同化系统数据产品（GLDAS-Noah）和欧洲中期天气预报中心发布的第五代再分析资料（ERA5-Land）进行了对比分析。结果表明,在该区域平均厚度为2.72 m的活动层内,土壤质量含水量（总含水量）约为14.0%,活动层土壤含水量与植被发育情况存在正相关关系。除高寒沼泽草甸类型外,高寒草甸与高寒草原类型的活动层含水量随深度的增加呈现出先减小后增大的变化趋势。不同坡位类型的活动层含水量呈上坡位>下坡位>中坡位>平坡位,阳坡水分高于阴坡且两者活动层剖面水分变化相似。多年冻土区浅表层0~350 cm深度范围内的土壤含水量大于区内融区同深度的土壤含水量,两者土壤剖面水分分布均呈现出先增大后减小再增大的特征。该区域的GLDAS-Noah同化水分产品与实测数据对比的误差在10%以内,比ERA5-Land再分析土壤水分数据更为准确,但两种数据产品对土壤剖面上的水分垂直分布情况描述均与实测数据有较大差异。该研究结果可以为数据同化系统的模式冻融参数化方案优化及遥感水分产品研发提供科学依据。     

隐伏斑岩铜矿区微细粒土壤地球化学特征及其找矿指示意义-以福建罗卜岭铜钼矿为例

在风化壳覆盖区应用传统化探方法和矿物地球化学找矿方法开展斑岩型矿床深部找矿存在一定制约。土壤微细粒分离测量技术是一种可应用于覆盖区找矿的穿透性地球化学勘查技术,在干旱地区已取得良好的应用效果,亟需开展多景观、多矿种应用试验。基于此,本文选择为红土风化壳覆盖的福建省罗卜岭斑岩铜钼矿为研究区,开展风化壳土壤微细粒分离测量技术有效性实验。结果显示,与不含矿酸性岩体风化壳相比,研究区明显富集铜钼矿成矿及伴生元素,尤其是Mo、Cu、Au显著富集,与此同时,含矿岩体风化壳中元素变异系数较低,符合斑岩型矿床元素含量富集系数高、变异系数低的典型特征。元素空间分布特征与矿化蚀变、断裂构造、地形演化密切相关。Cu-Mo异常可以圈定矿化中心蚀变带,V、Hg则在外蚀变带富集。Cu与Mo在研究区西北部的分异指示了紫金山矿田由SE向NW主成矿作用由Cu-Mo演变为Cu-Au。元素剖面分布特征进一步验证了成矿蚀变、地形演化对元素分布的控制作用。地形演化决定了蚀变带在地表的分布,进而控制元素的分布。罗卜岭铜钼矿体呈马鞍状分布于古背斜两翼,在后期风化剥蚀作用下,背斜核部演化为负地形,使得中心蚀变带接近出露地表,表现为Cu的正异常;古背斜两翼现今为正地形,矿体埋深较大,前缘晕元素Hg表现为正异常。因子分析结果很好地展示了元素的组合与分异特征。总体上,可以根据Cu-Mo异常判断矿体中心位置,根据Hg判断矿体埋深,根据Sn、V、Cr等识别花岗闪长斑岩体边界。本研究系统证明微细粒土壤测量在风化壳覆盖区探测隐伏斑岩铜钼矿的有效性。     

西藏纳木错表层沉积物中正构烷烃的来源与空间分布特征

通过测定纳木错现代植物和表层沉积物样品中的正构烷烃,结合主成分分析方法,明确了表层沉积物中烷烃的主要来源,并初步分析了它们在湖泊中的空间分布特征.研究结果显示,沉水植物所含烷烃以n-C21-C25为主,具有n-C23的峰值;陆生植物主要含n-C27-C33烷烃,具有n-C29或n-C31的峰值;但垫状点地梅(Androsace tapete)和香柏(Sabina pingii)的主峰碳却是n-C33烷烃,这在以往研究中鲜有报道.在纳木错表层沉积物中,正构烷烃具有典型的n-C31和n-C23双峰分布形式,来源于低等菌藻类、沉水植物以及陆生植物,其中碳数小于C20的短链烷烃主要来源于低等菌藻类,其空间分布均一,由低等菌藻类的浮游生活型所决定;长链烷烃主要来源于高等植物,含量从滨岸到湖中心逐渐减少,这与其在运移和沉积过程中受微生物的持续降解作用有关,此外,来源于陆生植物的n-C27-C33烷烃因河流汇水面积和流量不同而存在空间差异,这值得重视和进一步研究.     

Long interspersed elements in three species of Gracilaria （Gracilariaceae, Rhodophyta）

In order to find out whether long interspersed elements (LINEs) existed in macro-algae ge- nomes or not, we tested the LINE homologues in representative families (species): Gracilaria (G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii (Sur) De-Toni), Laminaria (L. longis- sima Miyabe and L. japonica Aresch.), and Ulva (U. lactuca L. and U. pertusa Kjellm.) during 2004 to 2005. Polymerase Chain Reaction (PCR) was carried out with degenerate oligonucleotide primers de- signed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly di- verged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlor- ella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs.     

New record of <Emphasis Type="Italic">Lobophora rosacea</Emphasis> (Dictyotales; Phaeophyceae) from the South China Sea

Lobophora rosacea C.W. Vieira, Payri et De Clerck is reported from the South China Sea for the first time. Our specimens are very similar to L. rosacea recently described from New Caledonia, not only in morphology but also in rbcL and cox3 gene sequences. The fan-shaped thallus grows erectly, attaching to the substrate by a basal holdfast. The thallus is composed of a single layer of large medullary cells and three to four layers of cortical cells on both sides of the medulla. Mature sporophytes are detected, with sporangium sori scattered on both surfaces of the thallus.     

基于MODIS的青藏高原湖泊透明度遥感反演

湖泊透明度是湖泊水体性质的一个重要参数,是湖泊浮游生物和进入湖泊的有机和无机颗粒溶解程度的综合反映,对湖泊生态环境研究具有重要的科学及实践意义。遥感影像是获取面积广、时间长的湖泊透明度的重要手段,但由于实测数据缺乏,目前对青藏高原地区湖泊透明度的遥感反演研究相对不足。本文基于青藏高原地区24个湖泊实测透明度SD(Secchi Depth)值和相应的MODIS遥感影像,建立了该地区湖泊水体透明度SD值MODIS遥感反演模型。结果表明：基于MODIS绿色波段B4的单波段幂函数模型在该地区反演效果最好,精度较高(R2=0.91, N=24),并具有较好的稳定性。以当惹雍错为例,选用该模型反演得到湖泊透明度的时间变化序列,发现该湖存在明显的季节波动和较为明显的年际变化。初步分析得出,降水/融水季节的湖泊透明度与湖泊所在流域的降水率具有密切的关系。本文结果表明,利用遥感手段能够有效地开展青藏高原地区湖泊透明度的反演,可为进一步深入研究该地区湖泊透明度及其影响要素奠定基础。