Properties of Klebsiella phage P13 and associated exopolysaccharide depolymerase

The bacteriophage P13 that infects Klebsiella serotype K13 contains a heat-stable depolymerase capable of effective degradation of exopolysaccharide(EPS) produced by this microorganism. In this study, the titer of phage P13, initially 2.0 × 107 pfu mL-1, was found increasing 20 min after infection and reached 5.0 × 109 pfu mL-1 in 60 min. Accordingly, the enzyme activity of depolymerase approached the maximum 60 min after infection. Treatment at 70℃ for 30 min inactivated all the phage, but retained over 90% of the depolymerase activity. Addition of acetone into the crude phage lysate led to precipitation of the protein, with a marked increase in bacterial EPS degradation activity and a rapid drop in the titer of phage. After partial purification by acetone precipitation and ultrafiltration centrifugation, the enzyme was separated from the phage particles, showing two components with enzyme activity on Q-Sepharose Fast Flow. The soluble enzyme had an optimum degradation activity at 60℃ and pH 6.5. Transmission electron microscopy demonstrated that the phage P13 particles were spherical with a diameter of 50 nm and a short stumpy tail. It was a double-strand DNA virus consisting of a nucleic acid molecule of 45976 bp. This work provides an efficient purification operation including thermal treatment and ultrafiltration centrifugation, to dissociate depolymerase from phage particles. The characterization of phage P13 and associated EPS depolymerase is beneficial for further application of this enzyme.     

A physics-based dual-frequency approach for altimeter wind speed retrieval

The altimeter normalized radar cross section(NRCS) has been used to retrieve the sea surface wind speed for decades, and more than a dozen of wind speed retrieval algorithms have been proposed. Despite the continuing efforts to improve the wind speed measurements, a bias dependence on wave state persists in all wind algorithms. On the basis of recent evidence that short waves are essentially modulated by local winds and much less affected by wave state, we proposed a physics-based approach to retrieve the wind speed from the dual-frequency difference in terms of the mean square slope of short waves. A collocated dataset of coincident altimeter/buoy measurements were used to develop and validate the approach. Validation against buoy measurements indicates that the approach is almost unbiased and has an overall root mean square error of 1.24 m s-1, which is 5.3% lower than the single-parameter algorithm in operational use(Witter and Chelton, 1991) and 2.4% lower than another dual-frequency approach(Chen et al., 2002). Furthermore, the results indicate that the new approach significantly improves the wave-dependent bias compared to the single-parameter algorithm. The capacity of altimeter to retrieve sea surface wind speed appears to be limited for the case of winds below 3 m s-1. The validity of the approach at high winds needs to be further examined in the future study.     

3种经典机器学习算法在火山岩测井岩性识别中的对比

岩性识别一直是火山岩油气勘探中的重要课题,基于测井数据的岩性识别也逐渐成为火山岩研究的需要,大数据背景下的机器学习算法为测井岩性识别提供了一个新方向。为提高某研究区火山岩岩性识别符合率,本文采用K近邻、支持向量机和自适应增强3种经典机器学习算法,对研究区内的粗面岩、非致密粗面岩、辉绿岩、辉长岩、玄武岩和非致密玄武岩等6类中基性火山岩进行岩性识别。从常规测井系列中优选对研究区岩性敏感的自然伽马、声波时差、补偿中子、深侧向电阻率和补偿密度等5种测井参数作为岩性识别模型的输入向量,从研究区内5口有岩心样品或薄片鉴定资料的目标层中选取测井数据点1 440个,其中960个作为训练样本,其余480个作为测试样本。以识别符合率和时间作为评价指标,对3种算法的识别结果进行对比分析,实验表明：自适应增强算法的分类准确率最高,6类岩性平均识别符合率达到82.10%;支持向量机算法表现良好,平均识别符合率为81.04%;K近邻算法平均识别符合率为76.04%。     

Geochemistry of Sandstones from the Silurian Hanxia Formation,North Qilian Belt,China: Implication for Provenance,Weathering and Tectonic Setting

The North Qilian orogenic belt is the key to figure out the evolution and assembly of Asia. The Upper Silurian Hanxia Formation which is deposited in the north area of North Qilian Orogenic Belt is of utmost important to reveal the architecture and orogenic process of the North Qilian belt. So provenance analysis of the Hanxia Formation is of significance to reveal not only that the tectonic evolution of the central orogenic belt China, but also that Paleozoic Asia plate reconstruction. The ratios of elements and some discrimination diagrams based on geochemistry indicate that felsic rocks constitute their main source rocks. The chemical index of alteration is less than 80, indicating that the source rocks are relatively fresh and of low maturity. On the Th/Sc versus Zr/Sc scatter plot, samples from Hanxia Formation occur along the magmatic compositional variation trend of rocks, indicating that the rocks did not undergo obvious sedimentary sorting and recycling. The major and trace elements discrimination diagrams imply that Hanxia Formation rocks were derived from continental island arc. Recent studies show that the North China plate subducted southwards and produced subduction-related arc magmatism along the southern margin of the North Qilian Terrane during the Silurian. Therefore, we infer that in the late Silurian period the subduction-related arc became accreted to the Central Qilian terrane to the south, forming a composite continental arc, and the North Qilian belt accumulated in a fore-arc basin.     

Wind wave characteristics and engineering environment of the South China Sea

Wave simulation was conducted for the period 1976 to 2005 in the South China Sea (SCS) using the wave model, WAVEWATCH-III. Wave characteristics and engineering environment were studied in the region. The wind input data are from the objective reanalysis wind datasets, which assimilate meteorological data from several sources. Comparisons of significant wave heights between simulation and TOPEX/Poseidon altimeter and buoy data show a good agreement in general. By statistical analysis, the wave characteristics, such as significant wave heights, dominant wave directions, and their seasonal variations, were discussed. The largest significant wave heights are found in winter and the smallest in spring. The annual mean dominant wave direction is northeast (NE) along the southwest (SW)-NE axis, east northeast in the northwest (NW) part of SCS, and north northeast in the southeast (SE) part of SCS. The joint distributions of wave heights and wave periods (directions) were studied. The results show a single peak pattern for joint significant wave heights and periods, and a double peak pattern for joint significant wave heights and mean directions. Furthermore, the main wave extreme parameters and directional extreme values, particularly for the 100-year return period, were also investigated. The main extreme values of significant wave heights are larger in the northern part of SCS than in the southern part, with the maximum value occurring to the southeast of Hainan Island. The direction of large directional extreme H _s values is focus in E in the northern and middle sea areas of SCS, while the direction of those is focus in N in the southeast sea areas of SCS.     

潘庄地区煤层气U型水平井技术工艺研究

我国已在多个地区进行了20余口煤层气U型水平井施工,但总体尚处于勘探实验阶段。为了研究潘庄地区煤层气U型水平井施工技术工艺,以寺河井田西区煤层气U型水平井为例,分析了实验区地质条件的可行性,重点研究了钻井技术工艺并初步考察了产气效果。结果表明：潘庄地区3#煤层含气量大、构造及水文地质条件简单,煤层产状稳定且厚度较大,原生结构煤体发育,比较适合U型水平井的施工。煤层气U型水平井具有技术工艺复杂、增产效果明显的特点,先进的工具仪器和技术工艺为煤层气U型水平井施工成功提供了技术保障,其最高产气量达到22 000m^3/d,相当于5-6口普通高产垂直井的产气效果,可为煤矿区煤层气抽采利用提供关键技术支撑。     

Preliminary measurements of boundary layer reactive halogens based on MAXDOAS technique over Salt Lake in West China

正The reactive halogens play key roles in the destruction of boundary layer ozone by catalytic reactions and provide a fast pathway to the sedimentation of elemental gaseous mercury.The presence of bromine oxides in the lower     

Submesoscale motions and their seasonality in the northern Bay of Bengal

The unbalanced submesoscale motions and their seasonality in the northern Bay of Bengal(BoB) are investigated using outputs of the high resolution regional oceanic modeling system. Submesoscale motions in the forms of filaments and eddies are present in the upper mixed layer during the whole annual cycle. Submesoscale motions show an obvious seasonality, in which they are active during the winter and spring but weak during the summer and fall. Their seasonality is associated with the mixed layer...