合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Calibration of JASON-1 Altimeter over Lake Erie

This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr.     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

基于GIS的柘林湾生态信息系统

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．     

Distribution and number change of the Littorinid(Gastropoda) on intertidal hard bed in the Jiulong Estuary, Fujian Province

-Among four species of Littorinid, Littorina scabra, L. brevicula, Nodiliitorina millegrana and N. pyramidalis pyramidalis, on the hard intertidal zone in the Jiulong Estuary, Fujian, L. scabra has the most extensive distribution both vertically and horizontally, and the highest anual average density. The uppermost limit of vertical distribution of N. pyramidalis pyramidalis is higher than the other three Littorinid. Desiccation is a main factor affecting the uppermost limit of vertical distribution of the Littorinid. The increasing wave action will raise the uppermost limit vertical distribution of Littorinid. With seasonal changes, the densities of the Littorinid also change in different tidal zonations. Difference in salinity is the main factor affecting the horizontal distribution of the Littorinid in the estuary.     

几种养殖鲍同工酶生化遗传的比较研究

运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。     

摄食水平和性别对稀有鮈鲫生长和能量收支的影响

于1995年10月采集本所自繁有ju鲫（约1.03g/ind)，在30℃水温下进行摄食－生长实验，研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响，结果表明，鱼体干物和能量含量不受摄食率和性别的影响，随着摄食率的增加，雌鱼的湿重，干重和能量特定生长率均呈双对数增加，雄鱼湿重特定生长率呈双对数增加，而干物质和能量特定生长率呈指增加，雄鱼生长速度及摄食率低于雌鱼，随着摄食率的增加，食物能损失于粪便中的比例均呈上升趋势，损失于排泄物中的比例变化不大，呈下降趋势，用于代谢的比例呈下降趋势，用于生长的比例呈上升趋势，除饥饿组外，在各个摄食水平，雌鱼食物能用于代谢的比例较雄鱼低，而用于生长的比例较雄鱼高。     

秦山核电站邻近水域浮游植物的生态研究

本文分析了秦山核电站邻近水域生态零点调查四个航次的浮游植物样品,结果表明:调查区浮游植物的种数和细胞密度在时间尺度上均存在明显的季节变化趋势:夏大于秋大于春大于冬,并且与环境因子的变化密切相关,其中最主要的影响因子是温度、盐度和径流,而影响日变化的环境因子主要是潮汐。     

PROBABILITY CHARACTERISTICS OF WAVE AND WAVE PRESSURES IN FRONT OF VERTICAL BREAKWATER

The distribution of wave height in front of a vertical breakwater, the range of wave pressure fluctuation at different subsurface levels, and the wave periods have shown that they are practically invariable with depth and can be determined theoretically. The distribution of crest and trough of total pressures is the same as that of wave height. As far as different subsurface levels are concerned, the spectral constitution of wave pressure field and the regularity of attenuation of spectral components are analyzed at the vertical breakwater, and a new expression describing the equilibrium range of wave pressure spectrum obtained.     

One new species of free-living marine nematodes (Enoplida,Anticomidae, Cephalanticoma) from the Huanghai Sea

One new species of free-living marine nematodes Cephalanticoma filicaudata sp. nov. from the Huanghai Sea, China is described and illustrated. Cephalanticoma filicaudata sp. nov. is characterized by well developed cephalic capsule, three teeth at the anterior of oesophagus, excretory pore opens posterior to cervical setae, only two cervical setae per file, body length longer than 5 200 μm, tail long and no ventral-lateral seta between the supplement and the cloacal opening. Types are deposited in the College of Marine Life Sciences, Ocean University of China.