Extensive genetic divergence among Diptychus maculatus populations in northwest China

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.     

2009-2013年普里兹湾表层水体中生物硅含量的年际变化及其与全球气候事件的关系

利用中国南极科学考察期间获得的颗粒物样品,对南大洋普里兹湾海域水体中生物硅的含量分布及年际变化情况进行了分析。研究结果显示:在2013年夏季普里兹湾表层水体中生物硅含量在0.38—8.62μmol/dm3之间变化,平均为1.55±1.86μmol/dm3。生物硅在67°S以南湾内区域表层水体中的含量明显高于67°S以北的湾外区域,最大值出现在普里兹湾陆架区,生物因子是表层水体生物硅含量分布的主控因子。根据不同年份即25、26、27、28、29次南极科学考察期间获取的数据研究显示,普里兹湾表层水体中生物硅含量存在明显的年际差异。历史数据分析结果表明,气候事件对普里兹湾海冰变化有明显的影响,因而对相应年份该海域浮游植物数量和种群结构也会产生一定的影响,从而导致表层水体中生物硅含量的年际差异。     

基于电子鼻的青蛤(Cyclina sinensis)软组织多氯联苯的快速检测方法研究

为建立一种简单快捷的水产品中多氯联苯(PCBs)检测方法,本研究应用电子鼻对青蛤(Cyclina sinensis)软组织中富集的多氯联苯进行检测,并采用HS-SPME-GC-MS(顶空固相微萃取-气质联用)对结果进行了验证。首先利用电子鼻制作PCB15浓度梯度模版,检测PCB15处理72h以及144h的青蛤软组织中残留量,采用LDA和PCA两种方法统计并同模板的浓度进行比较。结论:青蛤软组织中PCB15浓度能明显分开,并随着处理时间的延长,区分效果愈加明显,区分度愈大。PCA的分辨效果明显优于LDA。运用欧氏距离、马氏距离、判别函数法和相关性对含有PCB15的样品浓度分别进行鉴别和验证,四种方法总的鉴别率均达到90%以上,能够较准确地对样品进行验证。电子鼻检测青蛤软组织中的多氯联苯的最低检测限为0.05μg/L,GC-MS为0.5μg/L。     

南海北部陆架区两个台风过境时近惯性运动的若干特征

Features of near-inertial motions on the shelf(60 m deep) of the northern South China Sea were observed under the passage of two typhoons during the summer of 2009. There are two peaks in spectra at both sub-inertial and super-inertial frequencies. The super-inertial energy maximizes near the surface, while the sub-inertial energy maximizes at a deeper layer of 15 m. The sub-inertial shift of frequency is induced by the negative background vorticity. The super-inertial shift is probably attributed to the near-inertial wave propagating from higher latitudes. The near-inertial currents exhibit a two-layer pattern being separated at mid-depth(25–30 m), with the phase in the upper layer being nearly opposite to that in the lower layer. The vertical propagation of phase implies that the near-inertial energy is not dominantly downward. The upward flux of the near-inertial energy is more evident at the surface layer(17 m). There exist two boundaries at 17 and 40 m, where the near-inertial energy is reflected upward and downward. The near-inertial motion is intermittent and can reach a peak of as much as 30 cm/s. The passage of Typhoon Nangka generates an intensive near-inertial event, but Typhoon Linfa does not. This difference is attributed to the relative mooring locations, which is on the right hand side of Nangka's path(leading to a wind pattern rotating clockwise with time) and is on the left hand side of Linfa's path(leading to a wind pattern rotating anti-clockwise with time).     

山东半岛北部滨浅海区表层沉积物铝物源鉴别

Surface sediment samples in the near shore area of the north Shandong Peninsula are collected for grain size and element analyses. The results indicate that the surface sediments in the study area are primarily composed of the silt-sized components similar to the Huanghe River. The total concentration of aluminum varies from 5.57% to7.37%(average(6.33 ± 0.40)%), and its spatial distribution is mainly controlled by the grain size. Correlations between the ratio of aluminum to titanium concentration and aluminum concentration, titanium concentration and the mean grain size indicate that aluminum in the near shore surface sediments is affected majorly by the terrigenous source, and partially by the anthropogenic source. The ratios of aluminum to titanium concentrations are larger than the background value of loess matter at some stations due to the existence of excess aluminum associated with human activities. Thus, the sources of aluminum should be identified firstly when aluminum is used as an index of terrigenous matter even in the near shore area dominated by terrigenous deposits.     

中国南海一株固氮类芽孢杆菌的筛选和分离鉴定

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。     

地图审校和错误修改的定义及其应用

地物的性质和位置是地图表示的两个基本要素。制图者对地物的反映,即是制图者关于地物的观念模型和地图符号的超前表象。根据观念模型与地图符号的距离函数,定义地图符号的正确或错误。发现地图符号的错误并标注出来是地图审校的本质特征。地图符号的修改定义为地图符号从错误到正确的变换。地图审校和错误修改是传统和现代地图制图中确保地图质量的通用模式。     

数字滤波技术在潮汐观测中的应用研究

潮汐是重要的海洋物理要素。瞬时变化的海面高度信息除了包含潮汐信息外,还包括波浪等高频干扰信息,因此需要对干扰信息进行滤除。如何从获取到的海面瞬时高度变化中提取出潮汐信息就成为一个关键问题。海洋学中通常是通过低通滤波来获取低频的潮汐信息。通过分析常用的3种低通数字滤波的方法,即滑动平均法、快速傅里叶变换法和小波变换法,对3种低通滤波方法应用于潮汐数据处理的效果进行了比较分析,得出滑动平均法在潮汐信息提取中具有较高应用价值的结论。     

CHZ-Ⅱ海洋重力仪重力敏感结构的性能测试与分析

在原有CHZ重力仪研制基础上,设计新一代海洋重力仪CHZ-Ⅱ并进行硬件搭建与测试。阐述了为CHZ-Ⅱ重力敏感结构设计的测试方案、实验过程及其结果分析,主要包括弹性系统的刚度、交叉耦合效应和漂移效应。实验表明,该结构在目前的测试内容下符合设计要求。     

基于天地图的移动租房软件设计与实现

针对成都市传统中介租房带来的信息不畅、不透明,费用过高等问题,基于国产天地图提供的优质地理信息和服务编程接口,结合移动终端Android平台,设计了一款成都市手机租房软件"猎房宝",软件实现了租房查询、房屋详情查看、房源发布、路线查询、关注评论等功能。软件的特色是使用国产天地图解决租房问题,并设计了"好评度找房"的算法和功能。运行测试结果表明,该租房软件能较好地运行于基于Android操作系统的移动设备,为用户租房带来方便,具有一定的实用性。