The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish. 相似文献
Unlike conventional grouted micropiles, screw micropiles have been recently introduced to the foundation industry. Full-scale field tests of screw micropiles were carried out at a cohesive soil site. The screw micropiles have a diameter varying from 76 to 114 mm and a length varying from 1.6 to 3 m, and spiral threads welded on the lower half of the steel tubular shaft. Site investigation from cone penetration tests (CPT) and laboratory testing implies that the soil was medium to stiff, low plasticity clay. Six axial monotonic and three axial cyclic load tests were performed on three micropiles. One micropile was instrumented with strain gauges to investigate the shaft load distribution during loading. The axial cyclic loading was intended to simulate cyclic inertia load during vertical ground motions. Results showed that the micropiles behave as frictional piles during monotonic tests; the unit shaft resistance and adhesion coefficient were calculated and compared with results in the literature. The end installation torque was estimated using CPT shaft resistance and was shown to agree reasonably with the measured torque. Under axial cyclic loading, the micropiles underwent small cumulative displacements and the magnitude of the displacement decreased with increasing pile length and diameter. Cyclic loading redistributed the load transfer along different segments of the micropile. Negative skin resistance was observed along the smooth pile shaft when the pile underwent decreasing axial loading.