Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene

Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification(LAMP) technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.     

基于车载激光点云的街景立面自动提取

街道景观图是城市规划设计和城市管理的重要参考依据,车载点云数据能够提供沿街建筑的三维点信息,精度高,覆盖范围广泛,为街景立面整治提供了新的解决方案。为此,本文提出一种适用于车载点云的街景立面的自动提取方法,提取立面点云的具体步骤为：对原始数据去噪滤波;选取非地面点构建规则格网并二值化,依据语义特征筛选出建筑物点云;用POS数据拟合直线段帮助选取参考向量与参考平面;计算点云到参考面的距离,按距离分类点云数据,并对前述步骤中未分类点另行提取,合并面点集得到以沿街建筑物立面为主的街景立面点云。为了验证这一方法的可行性和有效性,采用点云数据进行实验,实验结果表明本方法在一定程度上提高了数据处理效率,能得到较理想的结果。     

招平断裂带中段深部结构构造——来自地震剖面的证据

玲珑花岗岩岩基东侧的招平断裂带是胶西北矿集区的主要控矿构造之一。布设于招平断裂带中段大尹格庄矿区的地震剖面,揭示了招平断裂带中段深部的结构构造特征,地震剖面显示,玲珑岩基内部有多个类似"拱弧构造"的岩浆侵入形成的弧形界面,由此推测存在2~3期的岩浆活动。剖面显示了3期构造组合样式,第1期为以招平断裂带和宋家河断裂为组合倾向SE的铲式断裂,底部似汇入统一的构造滑脱带;第2期为倾向NW的错断了招平断裂带的反倾伸展断裂;第3期为NE向延伸以花状构造为特征的走滑断裂。前两期反映构造发育处于伸展环境,第3期显示为左行走滑。招平断裂带深部延伸存在分支复合现象,形成由2个主裂面夹持的透镜状岩片。地震反射剖面数据的获得对深刻理解该区域中生代岩浆构造演化过程和深部找矿具有重要的借鉴意义。     

山东龙口市大磨曲家金矿地质特征及找矿方向探讨

大磨曲家金矿位于玲珑金矿田东风矿床主脉的NE延伸部位.矿区内已发现矿脉25条.通过对玲珑金矿田的成矿条件分析和东风矿床的综合研究,认为本区有很好的找矿前景.     

多连通域浮式平台波浪载荷水池模型试验研究

大型多连通域浮式平台的典型代表是大型海上旅游浮式综合体,依据概念设计,开展大型多连通域浮式平台的波浪载荷水池模型试验研究,试验对不同海况下4种典型多连通域浮式平台方案进行波浪载荷测量,包括剖面的垂向弯矩、水平弯矩和扭矩等,通过观察试验现象和分析测量数据,获得了不同方案大型多连通域浮式平台的波浪载荷特性。试验结果表明：由于平台质量大、振动阻尼大,所以高频载荷成分小;平台在自由状态下载荷值基本都小于平台只释放垂荡状态载荷值;在方案四高波高状态下,平台波浪载荷的高频成分包含了波浪的非线性、约束柱与平台之间的耦合、平台自身的高频振动等。研究成果可为大型海上旅游浮式综合体的结构设计提供设计载荷输入,支撑平台的强度校核评估。     

Field of vertical crustal deformation in North China and the feature of its dynamic evolution

ＦｉｅｌｄｏｆｖｅｒｔｉｃａｌｃｒｕｓｔａｌｄｅｆｏｒｍａｔｉｏｎｉｎＮｏｒｔｈＣｈｉｎａａｎｄｔｈｅｆｅａｔｕｒｅｏｆｉｔｓｄｙｎａｍｉｃｅｖｏｌｕｔｉｏｎ王若柏，洪汉净，许忠淮，耿世昌，孙东平，韩月萍Ｒｕｏ－ＢａｉＷＡＮＧ；Ｈａｎ－ＪｉｎｇＨＯＮ...     

Seismogenic model of earthquakes in groups in tectonic block and analysis for some features of earthquake precursory field

Ｓｅｉｓｍｏｇｅｎｉｃｍｏｄｅｌｏｆｅａｒｔｈｑｕａｋｅｓｉｎｇｒｏｕｐｓ　ｉｎｔｅｃｔｏｎｉｃｂｌｏｃｋａｎｄａｎａｌｙｓｉｓｆｏｒｓｏｍｅｆｅａｔｕｒｅｓｏｆｅａｒｔｈｑｕａｋｅｐｒｅｃｕｒｓｏｒｙｆｉｅｌｄＧｕｏ－ＭｉｎＺＨＡＮＧ；Ｌｕ－Ｍｉｎ...     

三维F－K域DMO方法及应用效果

在常速情况下，从三维时距方程出发，通过倾角代换，将三维倾角时差分裂为两个正交方向上的二维倾角时差，然后在这两个正交方向上分别做一次二维倾角时差校正，便可达到三维倾角时差校正的目的．应用实例表明这种三维ＤＭＯ分裂方法是切实有效的．     

Chemical variation from biolipids to sedimentary organic matter in modern oceans and its implication to the geobiological evaluation of ancient hydrocarbon source rocks

Understanding the dynamics of organic matter in modern marine water columns greatly favors the geobiological evaluation of hydrocarbon source rocks. Biolipids could make great contribution to petroleum hydrocarbons due to their comparable chemical components and the slightly refractory characteristics of biolipids during the microbial/thermal degradation. A variety of environmental factors such as temperature, CO₂ and salinity could affect the biochemical contents in microorganisms. As a result, microorganisms living in a changing environmental condition might have a different contribution to the petroleum formation. Organic carbon flux is shown to bear a positive correlation with the primary productivity only within a certain range of biomass volumes in a specific biohabitat. Furthermore, organic matter is degraded much quickly in a water column with oxic conditions. Therefore, the anoxic condition, along with the enhanced biological productivity, would be one of the significant factors in the formation of high-quality hydrocarbon source rocks. The formation of biofilms and microbial mats favors the preservation of sedimentary organic matter by decreasing the degradation rate of organic matter. Identification of biofilms and microbial mats in sedimentary rocks will thus greatly help to understand the depositional processes of organic matter finally preserved in hydrocarbon source rocks. __________ Translated from Earth Science—Journal of China University of Geosciences, 2007, 32(6): 748–754 [译自: 地球科学—中国地质大学学报     

火焰原子吸收光谱法测定铜精矿中银含量的测量不确定度评定

采用《测量不确定度评定与表示指南》，以火焰原子吸收光谱法测定铜精矿中的银含量为例，对测量结果进行不确定度评定。分析了不确定度的重要来源，包括称样质量、标准工作溶液、工作曲线拟合、试液定容体积及测量重复性等引入的不确定度分量组成。对各不确定度分量进行分析计算，求得标准不确定度为1．56，扩展不确定度为3．12。