Sediment records of environmental changes in the south end of the Zhejiang-Fujian coastal mud area during the past 100 years

Previous studies carried out in the East China Sea （ECS） mud area focused on long-term environmental changes in sedimentary records during the Holoeene, especially during the mid-Holocene high-stand water levels period. These results indicate that sensitive grain size groups can be used as a sedimentary proxy to reconstruct the evolution of the East Asian Winter Monsoon （EAWM）. The studies have been carried out mainly in the northern and middle portions of the Zhejiang-Fujian coastal mud, however, similar research in the southern portion and the comparison between sedimentary proxy and modern measured data of EAWM are lacking. In this paper, we focused on a sedimentary record of the past 100 years with an enhanced resolution of 1.8 years. Investigations of the southern end of the Zhejiang-Fujian coastal mud area were conducted on the basis of 21~Pb chronology, grain-size analysis and chemical element analysis. The correspondence between the mean grain size （Mz） of sediment sensitive grain size and the measured EAWM was confirmed for the first time. We found that during the recent 100 years, the variation of the mean grain size of the sensitive population in the southern portion of the Zhejiang-Fujian mud was mainly controlled by the EAWM intensity changes; and not directly related to changes in the sediment discharge from Datong station of the Changjiang River （DTSD）. Finally, recent changes in the content of heavy metals in study area reflect the impact of human activities on the environment.     

基于便携式闪电探测器的设计与实现

为了解决能在任何地方和任何时候探测闪电的问题,提出便携式闪电探测器设计的新方法。闪电探测器以MSP430F5438为控制中心,结合新型闪电传感器AS3935获取风暴前沿抵达探测器的估计距离。闪电探测器将距离和闪电发生的时间一起显示在Nokia 5110液晶模块上,并发出不同频率的报警声音,距离和时间信息也自动存入EEPROM。另外,闪电探测器带按键,实现人机交互,方便查看闪电发生的历史记录。经过试验证明,该闪电探测器具有探测准确性高、功耗低、成本低、体积小的优点,适合实际使用。     

不同防风标准雨量计的观测差异

针对不同的防风标准在翻斗雨量计观测时对风场变形误差的防护作用,从降水总量随风速波动的变化、设备间的均方差及其相关系数和观测时间灵敏度等几个方面对观测数据进行分析。数据分析表明不同的防风标准翻斗式雨量计对风场变形误差的防护作用存在一定的差异,其中SMALLDIFR具有更高的时间灵敏性,在雨量比较大时,偏斜式雨量计更优。     

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面向城市导游的无缝服务研究

智能导游服务已从传统的单一景区导游发展为城市多景区导游,因此,包含了景区间导游和景区内导游两大部分;表现为更加注重面向散客服务、兼容多移动智能终端以应用程序(App)的方式运行。由于景区间导游和景区内导游往往自成系统,在游客进出景区的过程中存在着两种系统的切换,且主要采用手动方式完成,尚没有实现城市多景区导游过程的无缝衔接。为了应对这个挑战,本文设计了一个面向城市多景区的无缝导游服务模式,分析了其架构以及工作机制;并着重讨论了城市无缝导游服务的关键技术,如系统间无缝切换的条件,包含判断游客进入、离开景区等;以及导游服务过程无缝衔接的机制,包含导游动态数据结构的设计,游客游览需求、导游过程状态在多导游系统间的传递等。最后通过实例验证了该方法的有效性,作为新的导游服务模式,同时为动态集成已有的导游App提供了解决方法。     

Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

闽北地区边界层移动风廓线雷达对比试验评估

为了更好地把握风廓线雷达的探测性能和数据精度,对移动风廓线雷达与L波段探空雷达资料进行对比统计分析,结果表明：移动风廓线雷达的有效数据获取率达到80％的高度为3500m,符合边界层风廓线雷达的有效探测高度。移动风廓线的径向速度平均差和标准差随着高度的增加而增加,东西方向的径向速度误差比南北方向的高约0．5—1．0m／s。风廓线雷达自身数据的准确性良好,但是降雨对数据的准确性影响比较大。这次对比试验结果表明,对比试验应该选择比较平稳的天气过程。由于秋冬季节大气环流比较稳定,降雨类型多为层状云降雨,因而风廓线雷达数据可靠性高;对流性降雨过程往往造成风廓线雷达资料可靠性降低。     

三疣梭子蟹(Portunus trituberculatus)CYP2基因的cDNA克隆及表达分析

通过RT-PCR及Smart?TM Race技术,首次克隆了三疣梭子蟹(Portunus trituberculatus)CYP2基因cDNA全长序列。该基因cDNA全长1662bp,编码一个由492个氨基酸组成的多肽,预测理论等电点为6.348,分子量大小为56.68kD。氨基酸序列中含有CYP基因家族所特有的K螺旋保守序列(ExxR)和血红素结合区(FxxGxxxCxG)。经氨基酸序列比对及系统进化树分析发现,与岸蟹(Carcinus maenas)的同源性最高,达到75%。实时荧光定量PCR结果表明,CYP2基因在肝胰腺、鳃、肌肉、血淋巴、心脏和眼柄中均有分布,在肝胰腺中表达量最高。肌肉注射磺胺嘧啶后,三疣梭子蟹高、中、低三剂量组CYP2基因表达较对照组都有上调,并具有时间差异性,低剂量组表达量逐渐降低,趋于对照组,中剂量组和高剂量组表达量先升高后降低,6h后同一时间点,均是高剂量组表达最高,低剂量组最低。表明磺胺嘧啶可诱导三疣梭子蟹CYP2基因,CYP2基因可能参与三疣梭子蟹的药物代谢反应。     

Dispersal of the Zhujiang River (Pearl River) derived sediment in the Holocene

High-resolution Chirp profiling and coring reveals an elongated(ca. 400 km) Holocene Zhujiang River(Pearl River)-derived mud area(maximum thickness 20 m) extending from the Zhujiang River Delta, southwestward off the Guangdong coast, to the Leizhou Peninsula. Two depo-centers, one proximal and one distal, are identified. On the continental shelf off the west Guangdong Province, the mud is deposited in water depth shallower than 50 m; while to the southeast of the Zhujiang River Estuary, the mud area can extend to the-120 m isobath. A combined analysis with the stratigraphic sequences of other muddy deposits in the Western Pacific marginal seas(mainly Changjiang(Yangtze) and Huanghe(Yellow) Rivers derived) indicates that the initiation of the Zhujiang River muddy deposit can be further divided into two stages: Stage 1 is before the mid-Holocene sea-level highstand(ca. 7.0 cal. ka BP), the proximal mud was mostly deposited after 9.0 cal. ka BP, when the sea-level rose slowly after the Meltwater Pulse-1C; Stage 2, after the mid-Holocene sealevel highstand, clinoform developed on the continental shelf off the west Guangdong Province, extending ca. 400 km from the Zhujiang River Estuary. The proximal clinoform thins offshore, from ca. 10 m thickness around 5–10 m water depth to less than 1–2 m around 20–30 m water depth. In addition, we also find a developed distal clinoform in the east of the Leizhou Peninsula.