Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65°C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.     

MVT铅-锌矿床的地球化学研究方法及其成矿系统探讨

本文总结MVT铅-锌矿床的最新地球化学研究方法及研究成果,包括δ18O、87Sr/86Sr、δ34S、206Pb/204Pb、Cl/Br、δ44Ca、同位素定年,以及利用地球化学参数所进行的数值模拟等。以这些方法的研究成果为依据,结合对白云石化作用和有机质在成矿系统中作用的分析,深入探讨了MVT铅-锌矿床的成矿系统,总结出一个与有机质密切相关的流体混合成矿假说。另外,强调了最新研究成果——金属硫化物和脉石矿物相异的流体包裹体成分,将在今后成矿系统的研究中发挥重要作用。     

乳品厂污水养殖螺旋藻的研究

探讨了螺旋藻在乳品厂污水中生长的生态条件及藻对污水中营养盐及有机质的去除效率。结果表明,低接种密度有利于藻的增殖。乳品厂污水需要经过一定的前处理。添另碳酸钠（ＮａＣＯ３）调节ＰＨ值并且适量添加尿素（ＣＯ（ＮＨ２）２）调节氮、磷比,最佳ＰＨ值为１０．６０,最佳氮、磷比为２１：１。在此条件下螺旋藻的蛋白质含量可达６７．３５％。污水中ＣＯＤ、ＰＯ４－Ｐ、Ｔ－Ｐ、Ｔ－Ｎ及ＮＨ４－Ｎ的去除率分别达到５４．８     

Lead isotope geochemical characteristics of Pb-Zn-Cu deposits on the southwestern margin of Tarim, and their significance

The polymetallic(Pb,Zn,Cu,etc) ore belt on the southwestern margin of Tarim is one of the major regions with the greatest prospecting potential in Xinjiang.Reported in this paper are the lead isotope data for 66 sulfide samples(including 50 galena samples,15 chalcopyrite samples and 1 pyrite sample) collected from such representative deposits as Tamu,Tiekelike,Kalangu,Abalieke,etc.in this ore belt.The Pb isotopic ratios of 206 Pb/204 Pb,207 Pb/204 Pb and 208 Pb/204 Pb in the galena samples range from 17.931 to 18.176,15.609 to 15.818 and 38.197 to 38.944,with the average values of 18.017,15.684 and 38.462,respectively.Those in the chalcopyrite samples range from 17.926 to 18.144,15.598 to 15.628 and 38.171 to 38.583,with the average values of 18.020,15.606 and 38.262,respectively.The pyrite sample has the Pb isotopic ratios of 206 Pb/204 Pb,207 Pb/204 Pb and 208 Pb/204 Pb to be 17.980,15.604 and 38.145,respectively.In combination with the previous Pb isotope data for sulfides,it is found that there is only a slight variation in the Pb isotopic composition of galena,chalcopyrite,sphalerite and pyrite in the ore belt.However,there is some difference in Pb isotopic characteristics between galena and chalcopyrite,especially the Pb isotopic composition of galena shows an obvious linear correlation with some other relevant parameters(e.g.β and γ).The comprehensive analysis suggested that lead in galena(maybe including sphalerite and pyrite) was derived principally from wall rocks and underlying basement,and that in chalcopyrite only originated from the basement.The single-stage model ages of these sulfides couldn’t indicate the time limit of metallogenesis(Pb,Zn,Cu,etc.),and the positive linear correlations for the Pb isotopic composition of galena are of no single-stage and two-stage Pb-Pb isochron significance.Furthermore,there are significant differences in Pb isotopic composition characteristics between the genetic type of deposits in this polymetallic ore belt and the Mississippi Valley type(MVT).In addition,the authors also pointed out that there is a phenomenon of differentiation(not paragenesis) for lead and copper elements during the process of metallogenesis in this ore belt.     

脾胃湿热证与气象要素相关性初探

利用湖南中医药大学附属二医院2008年消化内科脾胃疾病湿热内蕴证与同期长沙市气象要素观测资料,通过回归分析,对脾胃疾病湿热内蕴证与气象要素的相关性进行了研究。结果表明,湿热内蕴证与气温、降水量、相对湿度呈正相关,与气压呈负相关,与风无显著相关。     

基于自动观测站的鲁中地区土壤水分变化规律及精细化预报模型的研究

为了建立鲁中地区土壤水分精细化预报模型,利用2010—2013年农田土壤水分自动站逐日资料进行土壤水分年、月变化特征研究,并结合附近自动气象站资料,以土壤水分平衡方程、农田蒸散模型为基础,采用逐步回归和曲线估计等方法建立4—6月无降水条件下平原水浇田与山旱田土壤水分1 d、7 d降幅的经验预报模型。结果表明:鲁中地区0~100 cm土壤水分贮存量年变化趋势和0~50 cm基本一致,年最高出现在8月,最低出现在6月,年降幅最大出现在3—6月,易出现干旱。对预报模型进行回代和预报检验结果显示,回代平均相对误差为0.07%,7 d模型和1 d模型滚动预报第7天0~50 cm土壤水分贮存量,绝对误差分别为-0.15和-2.17 mm,平均相对误差分别为-0.07%和-1.56%,模型具有较强的理论基础和实用性,预报精度较高,为鲁中地区土壤墒情监测和精细化预报提供支持。     

成都地磁台的观测与评估

通过对成都地磁台的仪器配置、误差来源、资料质量等方面进行综合分析，结果表明．成都地磁台的观测质量稳定，资料连续可靠。成都地磁台的观测能够正确反映成都地区地磁场的变化特征。     

锯缘青蟹(Scylla serrata)幼体胃磨发育的比较研究

在青蟹幼体发育过程中，胃磨呈现出明显的变化。Ｚ１缺乏胃磨的结构，ＺⅡ开始出现中齿和侧齿的雏形，明显的可以识别的中齿和侧齿出现在ＺⅢ，．从ＺＶ开始胃磨已臻完善，除具有中齿和侧齿外，还出现了４个附属齿，包括两个附属的中齿和两个附属的侧齿。大眼幼体期，中齿和侧齿比ＺＶ更加尖锐，附属齿也更加锋利。在仔蟹前，中齿齿尖发生特化，形成垫状角质化区域，侧齿、附属齿更加锋利。这表明青蟹幼体发育过程中胃磨结构的变化与     

红土镍矿化学成分的相关性探析

红土镍矿进口量大、成分复杂、差异性大。为全面了解红土镍矿中各化学成分的相关性,以日照港为主,连云港、天津港为辅,从2013年1月—12月,按进港量多少,每月抽取11~25个样本,共228个。通过元素分析,检出主要元素19个,逐对分析相关性。发现地质作用造成的多元素共生关系,也有外部环境风化淋滤后的弱相关、不相关,甚至有风化淋滤过程中形成的强相关。     

东北三江平原几个钻孔剖面的孢粉组合分析及其意义

三江平原位于我国东北的边陲,系黑龙江、松花江、乌苏里江汇流而成的冲积平原。其位置在东经129°11′20″至135°5′10″,北纬43°49′55′至48°27′40″(图1)。