Molecular characterization and expression analysis of Lily-type lectin (SmLTL) in turbot Scophthalmus maximus,and its response to Vibrio anguillarum

A full-length lily-type lectin(Sm LTL) was identified from turbot(Scophthalmus maximus) in this study. By searching database for protein identification and function prediction, Sm LTL were confirmed. The full-length c DNA of Sm LTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The Sm LTL peptide is characterized by a specific β-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30–99; two of the repeats share the classical mannose binding domain(Qx Dx Nx Vx Y) while the third binding site was similar to other fish-specific binding motifs(Tx Tx Gx Rx V). The primary, secondary, and tertiary structures of Sm LTL were predicted and analyzed, indicating that the S m LTL protein was hydrophilic, contained 5.36% α-helices, 39.29% extended strands, 16.07% β-folds, and 39.29% random coils, and three β-folds. Quantitative realtime polymerase chain reaction(qPCR) analysis revealed that the Sm LTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of Sm LTL expression were observed in other tissues. The expression of Sm LTL in gill, skin and intestine increased at m RNA level after stimulation of V ibrio anguillarum, our results suggest that Sm LTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that Sm LTL is a member of the lilytype lectin family and the information reported here will provide an important foundation for future research on the role of this protein.     

山东费县上冶地区地热资源特征研究

山东省临沂市被授牌为"中国地热城"。区内地热资源较为丰富,开发利用时间长,产生了显著的经济和社会效益。通过研究临沂市费县上冶地区的地质背景、地热地质特征,探求该地区的地热资源形成、发育的机理,对于指导区域地热资源的有效开发具有重要的意义。上冶地区南部奥陶纪马家沟群地层直接出露,大气降水直接或者间接补给岩溶热储含水层。近SN向的上冶东岭断裂及其次级断裂与蒙山断裂沟通,地下水通过断裂构造、岩溶孔洞、裂隙等通道向深部径流,受到大地热流的传导加热,在形成和运移过程中不断地溶滤热储围岩的各种化学成分。而上覆地层热导率低,具有一定的保温作用,从而使得地热资源在该地赋存和聚集。深部岩溶热储类型为典型的受断裂构造控制的层状兼带状热储。     

黄土高原大理河流域水沙耦合模型应用研究

水沙模型是定量描述水沙关系及水沙规律的重要工具,现阶段国内外对于水沙模型的研究大都为基于某个典型流域的经验统计模型或基于流域大量基础资料的物理模型,极大限制了其使用范围及模拟精度.本文建立了结构与参数均具有物理意义的流域水沙耦合物理概念模型,其优点是物理概念清楚,模拟精度高,实用性强,易于深入研究泥沙基本规律.该模型将概念性水文模型和泥沙模型耦合,提出水流挟沙能力和土壤抗侵蚀能力概念,用对数曲线近似描述流域土壤抗侵蚀能力的空间变异性,在拜格诺河道水流悬移质泥沙公式基础上建立概念性沟蚀产沙公式,参照水流汇集相似性建立泥沙汇集演算公式.选取黄河中游大理河流域4个不同流域尺度的实际流域对模型进行应用检验,模拟结果表明,该模型的水流泥沙两部分均有很高的模拟精度,可以很好地模拟黄河中游地区不同流域尺度和年际尺度上的洪水过程和泥沙产生及输移过程,充分证明了该模型结构、参数和计算方法的合理性,可用于定量分析流域内各项水保措施的减水减沙效益及水沙关系变化趋势,对该模型的推广应用可做进一步分析研究.     

营养状态指数在长江下游小型浅水湖泊富营养化水平评价中的局限及改进建议

基于多个环境变量、以等权或者加权平均法获得的综合营养状态指数（Carlson指数：TSIc;修正后的Carlson指数：TSImc;综合营养状态指数：TLIc;湖库营养状态指数：EIc）得分是当前评价湖泊营养水平的主要依据,其值计算时先要获得基于单个环境变量的营养状态指数分值。若基于总氮（TN）、总磷（TP）和透明度（SD）等的单个理化指标与基于叶绿素a(Chl.a)的营养状态指数得分间存在显著差异,表明依据理化指标的评估结果低估或者高估湖泊的实际营养水平。长江下游分布大量的小型浅水富营养化湖泊,但基于理化指标的评估结果是否会误判湖泊的实际富营养化水平的研究很少。本研究以长江下游的14个浅水富营养化湖泊为对象,于2019 2020年按照季度采集水样并测定水环境因子,分析基于理化指标和基于Chl.a的各营养状态指数（TSI、TSIm、TLI和EI）得分间的差异。结果表明,基于SD的TSI、TSIm、TLI和EI的年均得分均显著高于基于Chl.a的相应营养状态指数的年均值;除TSI(TP)vs.TSI(Chl.a)外,基于TP与基于Chl.a的其他营养指数的年均得分间均有显著性差异;仅TL...     

Response of soil erosion and sediment sorting to the transport mechanism on a steep rocky slope

A deeper understanding of the sediment characteristics associated with rock fragment content can improve our knowledge of the erosional processes and transport mechanisms of sediments on steep rocky slopes. This research used simulated rainfall experiments lasting for 1 h at a rate of 90 mm h⁻¹ and employed 5 × 1 × 0.4 m parallel troughs filled with purple soils with different rock fragment volumetric contents (0, 5, 10, 20, 30 and 40%) on a 15° slope gradient. For each simulated event, runoff and sediment were sampled at 1- and 3-min intervals, respectively, to study, in detail, the temporal changes in the size distributions of the eroded sediments. The results show that sediment concentrations, soil erosion rates and soil loss ratios significantly decreased as rock fragment content increased for rock fragment contents from 0 to 40% in purple soils. During the transportation process, clay particles often formed aggregates and were then transported as larger particles. Silt particles were more likely to be transported as primary particles with a low degree of sediment aggregation. Sand-sized particles, which constituted a greater proportion of the original soil than the eroded sediments, were formed from other fine particles and transported as aggregates rather than as primary particles. Suspension-saltation, which mainly transports fine particles of 0.02–0.05 mm and coarse particles larger than 0.5 mm in size, was the most important transport mechanism on steep rocky slopes. The results of this study can help to explain the inherent laws of erosional processes on steep rocky slopes and can provide a foundation for improving physical models of soil erosion. © 2019 John Wiley & Sons, Ltd.     

基于围填海的江苏用海承载指数评价研究

为进一步加强海域使用管理,促进海域开发利用和资源环境的可持续发展,文章建立围填海用海承载指数评价指标体系和评价模型,分析江苏13个县级行政区的围填海用海承载指数及其监测预警情况,并提出建议。研究结果表明:江苏围填海用海承载指数评价指标体系包括资源禀赋、开发利用强度、发展潜力和区域约束4个一级指标以及10个二级指标;江苏13个县级行政区的围填海用海承载指数为-0.46～9.63,呈显著的空间差异,其中中部辐射沙洲区的大丰区、东台市和如东县围填海用海承载条件良好,北部连云港市辖区以及南部南通市的通州区和启东市围填海用海承载条件较差;未来应建立围填海用海承载能力监测预警长效机制、严格实行围填海管控制度、合理调整和优化产业布局以及加强政府和社会的协同监督。     

基于ArcGIS 9.0的DEM的生成及坡度分析

介绍了利用地理信息系统软件ArcGIS 9.0来构建DEM的方法,并对生成的荆江分洪区DEM进行了坡度分析.DEM还可应用于区域规划、城镇规划和土地评估等工作中.实验证明,这种方法除了地形图的扫描外,其他过程都在ArcGIS 9.0软件中完成,不需要投入大量设备,成本低,速度快,容易更新,并可按工程的规模实时组织进行.     

基于ArcGIS 9.0的DEM的生成及坡度分析

介绍了利用地理信息系统软件ArcGIS 9.0来构建DEM的方法,并对生成的荆江分洪区DEM进行了坡度分析。DEM还可应用于区域规划、城镇规划和土地评估等工作中。实验证明,这种方法除了地形图的扫描外,其他过程都在ArcGIS 9.0软件中完成,不需要投入大量设备,成本低,速度快,容易更新,并可按工程的规模实时组织进行。     

中国特别重大自然灾害损失统计的最新进展——《特别重大自然灾害损失统计制度》解析

以《特别重大自然灾害损失统计制度》(简称《统计制度》)主要执笔人的视角,详细阐述了制度的主要内容、蕴含的科学问题和未来研究方向。《统计制度》报表和指标设计在与现有国家标准保持一致、历经汶川地震等多次重特大灾害实践检验完善、充分吸收相关行业部门意见和建议、适用于多灾种与灾害链损失统计、实用于灾后恢复重建规划编制等方面均体现出其权威性与最新性,《统计制度》具备科学性、综合性、实用性和动态性等特征。《统计制度》在推进特别重大自然灾害损失综合评估、启发涉灾行业部门开展专项深入研究、引领地方建立相关制度等方面具有极大潜力。     

黑潮入侵南海的强弱与太平洋年代际变化及厄尔尼诺-南方涛动现象的关系

在黑潮入侵南海强弱的问题上,到底是太平洋年代际变化(Pacific Decadal Oscillation,PDO)还是厄尔尼诺-南方涛动(El Nio-Southern Oscillation,ENSO)现象在起关键作用,目前还存在着较大争议。本文先以高盐水作为黑潮入侵强弱的示踪物,用120°E断面的高盐水数据和北赤道流分叉点(North Equator Current Bifurcation,NEC-Y)的南北变动进行相关分析,接着,进一步用学者所用的黑潮入侵指数(KI指数,Kuroshio intrusion index和NEC指数,North Equatorial Current index)与北赤道流分叉点南北变动进行相关分析。最后,用EMD(Empirical Mode Decomposition)方法和相关关系分析法分别分析了PDO指数、Nio3.4指数与北赤道流分叉点南北变动的关系并用NECP风场数据探讨其影响机制。结果表明:(1)通过对120°E断面的高盐水的KI指数、NEC指数与NEC-Y的相关分析,表明了北赤道流分叉点的南北变动能够很好地指代黑潮入侵南海的强弱;(2)通过PDO指数和Nio3.4指数与北赤道流分叉点的南北变动的相关性分析,发现PDO指数、Nio3.4指数与北赤道流分叉点的南北变动都具有较好的相关性,都在0.5水平。这些良好的相关性表明了PDO和ENSO对黑潮入侵南海的强弱都具有重要的影响;(3)当处于厄尔尼诺年(拉尼娜)时,赤道太平洋发生西(东)风异常,使得北赤道流分叉点偏北(南),使吕宋岛东侧的黑潮流速减弱(加强),黑潮入侵南海增强(减弱);当PDO处于暖(冷)阶段时,会加强热带太平洋的西(东)风异常,使得黑潮入侵南海增强(减弱)。