Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol(1-PEA)is a flavor extensively used in the production of co smetics,beverages,and food.The release of 1-PEA into coastal environments has aroused great concern.However,its potential effects on marine organisms are still unknown.In order to provide a better understanding of the ecological risks of 1-PEA in marine environments,this study determined the toxic effects of 1-PEA on two marine diatoms(Phaeodactylum tricornutum and Skeletonema costatum).The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h.The contents of chlorophyll a,chlorophyll c,glutathione(GSH),malondialdehyde(MDA),and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx),were measured at the end of the exposure period.1-PEA was shown to significantly inhibit the growth of diatoms,with 96-h EC_(50) values of 257.14 mg/L and 126.46 mg/L in P.tricornutum and S.costatum,respectively.In P.tricornutum,the levels of SOD,CAT,GPx,GSH,and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC_(50)value.However,in S.costatum,the activities of SOD and CAT,and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC_(50) value.Taken together,these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

海萝抗氧化酶系与失水响应因子表达模式分析

潮间带红藻海萝(Gloiopeltis furcata)对失水胁迫具有很强的耐受能力。为探索海萝周期性失水过程中的响应机制,本研究在24 h内设计了两次连续的失水-复水循环处理,测定了海萝抗氧化酶活力的变化情况。同时,利用转录组测序技术,结合荧光定量PCR方法(qRT-RCR),对海萝失水响应基因的转录表达进行了验证。结果表明:海萝转录组共组装到32681条基因。与对照组相比,处理组共表达了7161条差异表达基因(differentially expressed genes, DEGs)。KEGG分析显示, DEGs分布在代谢、环境信息加工、有机体系统、遗传信息加工、细胞进程等方面。海萝抗氧化酶活力测定发现,抗氧化能力对海萝响应失水胁迫十分重要。过氧化氢酶(CAT)、硫氧还蛋白还原酶(TrxR)、超氧化物歧化酶(SOD)参与抗氧化过程,其中CAT酶活力对海萝抵抗失水胁迫尤为重要。此外,qRT-PCR结果显示,海萝中渗透调节物质红藻糖苷合成的相关基因(GfUGPase、GfGK、GfGPDH)只对初次失水处理有正响应。而热激蛋白70基因(GfHSP70)、碳酸酐酶基因(GfCA)、MYB蛋白编码基因(GfMYB)以及谷胱甘肽S转移酶基因(GfGST)在两次失水过程中其转录水平均有上调表达,它们也参与了海萝失水响应机制。     

锁块式水下采油树井口连接器性能分析

水下采油树井口连接器是连接采油树和井口的关键设备,对深海水下勘探开发及采油树的安装连接具有不可或缺的重要意义。以一种锁块式水下连接器为例,介绍其结构组成和工作原理,通过有限元建立连接器的三维模型,分析连接器在下放安装工况、连接锁紧工况、正常生产工况和钻完井工况下的连接性能,得出井口与采油树本体接触面作用力、VX钢圈接触应力和锁块受力情况随外部载荷的相互关系。结果表明,VX钢圈在整个过程中都能够保持良好密封,井口与采油树本体接触面未发生分离,弯矩对连接器连接性能影响很大,在额定弯矩作用时连接器部分锁块已发生弹塑性失效。     

决口海堤水波传播模拟和分析研究Ⅱ:2D传播

应用经验证的SWASH数学模型,分析了海堤决口中心线沿线实测水深分布规律和保护区淹没水量与淹没面积。对不同特征水深进行验证,进而探讨不同波要素对不同形式的海堤决口堤后特征水深分布情况以及波浪传播距离与时间的关系;分析了物理模型试验中海堤决口宽度以及不同波要素对堤后洪水波演进的影响;计算了不同决口深度和宽度对洪水波传播的影响,建立堤后水体淹没水量和淹没面积分布公式。成果可供我国沿海城市和地区在风暴潮和台风浪作用下海堤决口风险图绘制参考。     

疏肝消瘿方联合西药治疗甲状腺功能亢进症40例临床观察

目的：观察疏肝消瘿方联合西药治疗甲状腺功能亢进症（甲亢）的临床疗效。方法：将80例甲亢患者随机分为治疗组和对照组,每组各40例。对照组给予甲巯咪唑治疗,治疗组在对照组的基础上给予疏肝消瘿方治疗,2组均以4周为1个疗程,随访12周后,比较2组患者的临床疗效、甲状腺功能及治疗前后服用西药剂量的变化。结果：总有效率治疗组为92.5%,对照组为75.0%,2组比较,差异有统计学意义（P<0.05）;2组TSH、FT3、FT4及TRAb水平治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P<0.05）;2组甲巯咪唑服用剂量治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P<0.05）。结论：疏肝消瘿方联合西药治疗甲状腺功能亢进症疗效优于单用西药组,值得临床推广应用。     

Bioactive substances of various seaweeds and their applications and utilization

Over the past century or more,traditional uses of seaweeds began and initially comprised the simple collection,sorting and drying of seaweeds harvested from the wild,or cast upon the beach,for use as soil“manure/fertilizer/improver”.Some macroalgae were used for unprocessed food purposes and subsequently harvested and cultivated biomasses of selected brown and red seaweeds becameindustrially processed to yield alginates,mannitol,iodine,agar,agarose,carrageenan,and more recently biostimulants,etc.Products from various seaweeds are very diverse and are commonly applied in a range that includes textiles and dying,food/feed and supplements and specialized medical applications.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

加权空-谱联合保持嵌入的高光谱遥感影像降维方法

高光谱遥感影像数据量大、波段数多,容易导致“维数灾难”。传统流形学习方法一般仅考虑其光谱特征,忽略了空间信息。为此提出一种非监督的基于加权空-谱联合保持嵌入(WSCPE)的维数约简算法。首先采用加权均值滤波(WMF)方法对高光谱影像进行滤波,以消除噪点和背景点的干扰。然后根据遥感影像地物分布的空间一致性,通过采用加权空-谱联合距离(WSCD)来融合像素点的光谱信息和空间信息,有效选取各像素点的空-谱近邻,并根据像素点与其空-谱近邻点之间的坐标距离来有区别的利用其近邻点进行流形重构,提取低维鉴别特征进行地物分类。在PaviaU和Indian Pines数据集上的分类结果表明,总体分类精度分别达到了98.89%和95.47%。该方法在反映影像内部流形结构的同时,有效融合了影像的空间-光谱信息,故能提高影像特征的鉴别性,并提升分类性能。     

Preparation and characterization of agar,agarose,and agaropectin from the red alga Ahnfeltia plicata

Agar,agarose,and agaropectin were extracted from the red alga Ahnfeltia plicata,and their properties and structures were characterized.Agar was extracted by a comparatively low alkaline consumption of 1.2%.It exhibited a gel strength of 1 152.50±74.25 g/cm^2 and a sulfate content of 0.55%±0.08%.The yield of agar from A.plicata was 24.53%,which is higher than those of other agarophytes commonly used in China.Three kinds of the method were compared for the purification of agarose,and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose.Furthermore,agaropectin was purified from A.plicata and characterized by GC,HPLC,UV-spectrum,and FI-IR to understand its composition and structure.It was the first time to comprehensively study the agar and its fractions from the red alga of A.plicata.This research provided an eco-friendly agar extraction method from A.plicata and revealed its potential application for the production of agar,agarose,and agaropectin.     

GNSS接收机DCB短期时变特征分析和估计方法

提出一种使用非差非组合精密单点定位(PPP)估计和分析接收机DCB短时时变特征的方法。首先利用非差非组合PPP得到包含接收机DCB的重构电离层参数估值;然后通过IGS电离层GIMs格网模型内插剥离各历元站星斜向电离层距离延迟;最后通过最小二乘约束得到各历元接收机DCB解。由于格网本身精度(2~8 TECU)和插值精度限制,解算出来的接收机DCB并不能真实反映其短期时变特征。为此,提出利用站间单差或者历元间差分的方法还原其真实的变化态势。实验结果表明,所提出的方法能够正确估计接收机DCB,并能真实还原其短期时变特征,具有良好的适用性。