An experimental study on major element release from the sediments in the Changjiang (Yangtze River) Estuary

With the enhanced warming and acidification of global ocean, whether and to what extent the naturally-weathered fluvial sediment into the sea can release elements and thus influence the geochemical pro...     

Extensive genetic divergence among Diptychus maculatus populations in northwest China

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.     

DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea

A total of 142 specimens of Ceramiales(Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species,14 genera,and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers,namely,partial large subunit r RNA gene(LSU),universal plastid amplicon(UPA),and partial mitochondrial cytochrome c oxidase subunit I gene(COI). No intraspecific divergence was found in our collection for these markers,except for a 1–3 bp divergence in the COI of Ceramium kondoi,Symphyocladia latiuscula,and Neosiphonia japonica. Because short DNA markers were used,the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in Gen Bank or BOLD(Barcode of Life Data) Systems. Three specimens were presumed as H eterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification,which was the first record in the investigated area,implying that it might be a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers,H eterosiphonia japonica converged with D asya spp. and was distant from the other Heterosiphonia spp.,implying that H. japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species,but the COI had a higher ability to discriminate between species.     

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野外型断层活动连续监测系统

介绍野外型断层活动连续监测系统的组成、布设及主要技术指标。     

SS-Y型伸缩仪安装中应注意的问题

对SS-Y型伸缩仪在运行与安装中需要注意的几个问题进行了探讨。     

Metabolism of pectenotoxins in brown crabs <Emphasis Type="Italic">Cancer pagurus</Emphasis> fed with blue mussels <Emphasis Type="Italic">Mytilus edulis</Emphasis>

We investigated the metabolism of pectenotoxins in brown crabs(Cancer pagurus).The crabs were fed with blue mussels(Mytilus edulis) for 21 d then depurated for 42 d.We extracted the toxins from the digestive glands of contaminated crabs,uncontaminated crabs(control group),and the meat of blue mussels using methanol.Extracts of the crab digestive glands were fractionated by liquid-liquid partitioning and solid phase extraction.The fractions were analyzed by liquid chromatography coupled with tandem mass spec...     

Ingestion of <Emphasis Type="Italic">Brachionus plicatilis</Emphasis> under different microalgae conditions

The effects of four microalgae,Chlorella vulgaris,Platymonas helgolandicavar,Isochrysis galbana,and Nitzschia closterium on the grazing and filtering rates of the marine rotifer,Brachionus plicatilis,were evaluated under laboratory conditions.The grazing rates in separate cultures of the four microalga were as follows:C.vulgaris > P.helgolandicavar > I.galbana > N.closterium.However,the filtering rates occurred in the following order:P.helgolandicavar > N.closterium > C.vulgaris > I.galbana.A mixed diets ex...     

Isolation and identification of bacteria associated with the surfaces of several algal species

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on ...