STUDY ON GAMETOPHYTE VEGETATIVE GROWTH OF UNDARIA PINNATIFIDA AND ITS APPLICATIONS

When cultured under certain environmental coriditions (25℃, light intensity 80 μmol/m~2 ·s, LD12/12, in enriched seawater medium with 7 × 10~(-4)mol/L NO_3,-N, 1.56× 10~(-4)mol/L, PO_4-P and supplementsof other elements like Mn, Fe, I, etc.), mate and female gametophytes of U pinnatifida keptgrowing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. Theempirical formula G_m=G_o. 3~m was established to estimate the output of vegeative gametophytes. Vigorousvegetative gametophyte cells began to form reproductive structures (oogonium and spermatangium, whenthe tmperature was lower than 25℃ and other environmental factors wrre kept optimal. The sufficientsupply of gametophyte cells provided enough seeds for raising Undaria sporelings on prodiction scale.Controlled cross-breeding experiments using selcted male and female gametophyte clones which increasetheir cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes fromthe cro     

Effect of nitrate and phosphate on accumulation of β-carotene isomers inDunaliella salina

Dunaliella salina, a halotolerant unicellular green alga, can accumulate a large amount of β-carotene under certain environmental conditions. The isomers of β-carotene extracted fromD. salina cultured in medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-T alumina column. At least six isomers were found in different proportions depending on the culture media's nitrate and/or phosphate concentrations. Nitrate and/or phosphate deficiency was conducive to the accumulation of total cis isomers but not of all trans isomer. It is suggested that 1 mmol/L KNO₃ and 0.1 mmol/L KH₂ PO₄ are favourable for accumulation of total cis β-carotene. Contribution No. 2090 from the Institute of Oceanology, Chinese Academy of Sciences. This project was supported by the National Natural Science Foundation of China, Grant No. 38970587.     

断层显微分辨率及其地震解释方法

探讨了地震资料断层解释技术,提出了断层可视分辨率和显微分辨率的新概念,这有助于统一地质、物探和采矿专家的意见。文中指出:在现有分辨率的地震资料基础上,经过解释处理及显示新技术,可以解释部分2~5m断距的断层。      

间歇施肥对龙须菜的生长和化学组成的影响

于１９８４年５－７月在青岛麦岛氮营养盐贫瘠的海区,对人工养殖的龙须菜进行间歇施肥现场实验。结果表明,龙须菜可以在短时间里快速吸睡氮盐并累积在藻体内以用于生长;经间歇施肥的藻体生长较快,产量较对照组的高８８％;藻化学组成分析结果显示,龙须菜体内含氮化合物和碳水化合物组成呈现负相关变化,存在氮低糖高,糖低氮高的现象。以上结果提示对氮盐贫瘠海区人工养殖的龙须菜间歇施肥,可以促进藻体生长,提高产量;在养殖     

煤层气储层参数多信息综合定量预测方法

提出了以地震属性和钻孔测井参数为基础, 以数学地质为桥梁, 实现煤层气储层参数定量预测的科学方法。薄煤层厚与反射波振幅之间为单调增减关系公式; 孔隙度与地震反射瞬时频率成正比关系, 可采含气量与地震反射瞬时振幅成反比关系, 且相关程度高。最后应用该成果对山西沁水煤层气地震勘探工程实际预测, 对储层厚度、孔隙度、含气量做出定量预测。该成果有利于提高煤矿采煤安全性和降低煤层气开采的风险性, 同时也将开拓地震勘探技术新的应用领域。      

贵州"西电东送"电煤保障建议

分析了贵州省“西电东送”火电建设中电煤保障方面存在的突出问题:矿井建设电煤资源保障存在缺口,当前矿井建设中地质勘查依据不足,从而增大了矿井建设投资风险并为生产事故多发和资源浪费埋下祸患,煤矿采矿及管理人才匮乏等。针对存在的问题提出了建议,供制定规划和领导决策参考:(1)贯彻落实国务院《关于加强地质工作的决定》,加强、加快贵州省煤炭地质勘探工作;(2)加快贵州省大中型煤矿建设,加强对小煤矿的管理、监督、引导和资源整合重组;(3)加快煤矿采矿和地质技术人才培养,特别是中等实用技术人才的培养。     

海带单倍体无性繁殖系育苗技术的研究

海带(Laminaria japonica)是一种经济褐藻,它的用途很广,在医药、食品、化工等方面都有广泛的应用.它是海洋生物中含碘量最高的一种,食用海带可以补碘,治疗甲状腺肿大病.我国是一个拥有十几亿人口的大国,当前约4亿多人口严重缺碘,因此海带养殖是关系到国计民生的大事.多年来在国内外人工养殖的藻类中,海带的产量一直居于首位.我国海带的年产量现已达30万t多干品[1],是海水养殖的支柱产业之一.养殖业的发展必须有充足、优质的苗源做保障,因此先进稳定的人工育苗技术至关重要.我国现在普遍采用的海带人工育苗技术创建于20世纪50年代,即“夏苗培育法”.