褐藻纲海带目Laminaria属和Saccharina属基于三个分子标记的系统发育分析

In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae–Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I(COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer(ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.     

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壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。     

限量灌溉对冬小麦抗旱增产和水分利用的影响

小麦体积含水量是表征小麦生理状态、影响小麦产量的重要参数,传统的测量手段如人工采样烘干、卫星遥感等,难以实现小麦体积含水量的连续、动态、高精度监测,由此提出了一种利用全球导航卫星系统（global navigation satellite system,GNSS）折射测量技术反演小麦体积含水量的方法。首先,使用两组GNSS天线同时接收直射信号和穿透小麦植株层的折射信号,利用两信号的振幅比表示折射信号的衰减程度;然后,基于电磁波传播理论,建立了GNSS直射、折射信号的物理模型,并在此基础上导出小麦体积含水量、振幅比、小麦高度、气温的函数关系,进而构建了反演小麦体积含水量的数学模型。利用导航型GNSS设备采集的数据对所提方法进行了验证,结果表明,在小麦拔节期、抽穗期和成熟期,该方法反演的小麦含水量与人工实测数据符合较好;当小麦含水量在1~7 kg/m³范围内时,反演结果的平均误差、标准差和均方根误差分别为0.058 kg/m³、0.396 kg/m³、0.399 kg/m³。研究结果证明了利用导航型GNSS接收机准确获取植被含水量动态变化信息的可行性,为低成本、低功耗、小型化GNSS植被含水量监测设备的研制奠定了基础。

     

科尔沁沙地粗—细组分的碎屑锆石U- Pb年龄特征：对定量物源及区域构造- 岩浆演化事件的指示

追踪干旱—半干旱地区大型沙地的物源和风- 河流相互作用机制对于理解陆地景观格局演变、地表过程与地貌动态以及大气圈和岩石圈之间的联系至关重要。然而,目前科尔沁沙地的物源仍存在较大争议,且缺乏具有统计意义的锆石U- Pb年龄数据库。因此,本研究对科尔沁沙地地表风成沙进行多点取样,根据不同粒级(＜63 μm和＞63 μm)选取1500颗碎屑锆石进行U- Pb测年分析,并利用逆向蒙特卡罗模型对其物源进行定量约束。结果表明,从目视定性的角度看,科尔沁沙地的碎屑锆石U- Pb年龄谱特征非常相似。但定量重建结果显示沙地的物源整体上以中亚造山带的贡献为主(50. 5%~61. 3%),然而东南部体现出华北克拉通的绝对优势(~75. 8%)。科尔沁沙地的物源存在空间异质性,沙地西部和北部的锆石年龄谱极为相似,与南部锆石年龄特征显著不同。科尔沁沙地的碎屑锆石U- Pb年龄特征基本不受粒度分异的影响,但沙地东南部除外。本文认为,风与河流的协同作用及其由此导致的沉积分异和再循环作用解释了科尔沁沙地碎屑锆石的U- Pb年龄特征。结合区域构造演化历史,科尔沁沙地在~2. 5 Ga和~1. 85 Ga的U- Pb年龄峰值分别是前寒武纪华北克拉通的生长、拼合与碰撞过程中的两期构造事件的产物。此外,~1. 7 Ga的锆石年龄可能是对Columbia超大陆聚合与裂解的综合响应。古生代以来的锆石年龄峰值(500~400 Ma、300~250 Ma、130~110 Ma)记录了在古亚洲洋的俯冲闭合、蒙古- 鄂霍次克海俯冲碰撞和古太平洋的俯冲、回退的区域构造背景下多期区域构造- 岩浆事件。     

在海洋环境中的酵母菌分布与多样性

最近几年作者对不同海洋环境中的酵母资源、分布和多样性进行的大量研究,从中发现了22属63种1051株。并且发现这些广泛分布在海水、海泥、高盐海水、深海、各种大型海藻表面、各种海鱼体表和体内和红树林生态系统中。不同种的酵母可以产生蛋白酶、脂酶、植酸酶、菊糖酶、纤维素酶、β-1,3-葡聚酶、嗜杀因子、核黄素、铁载体等活性产物,所以这些酵母菌具有广泛的实际用途。     

渤黄海沿海2月份海平面异常偏高成因分析

使用1980—2010年的水位、气温、海温、气压和风场资料,对中国渤黄海沿海2月份的海平面变化特征以及异常偏高成因进行了探讨,分析结果表明:近30年,渤黄海沿海2月份海平面呈现明显的上升趋势,2009年和2010年2月份的海平面达到近30年的高值,冬季高海平面导致全年平均海平面偏高。近两年2月份海平面处于多个长周期振动的高位重合期,各振幅叠加的结果近100 mm,对海平面起了明显的抬升作用。高海平面使得辽宁、河北以及山东等沿岸地区的海水入侵距离和土壤盐渍化程度均有所增加,海岸侵蚀加重;上海在2009—2010年连续2年2月份发生了近年较严重的咸潮入侵。2009年和2010年2月份,气压较常年同期显著偏低,冬季季风显著偏弱,是海平面上升的主要原因之一。     

大黄鱼病原副溶血弧菌单克隆抗体制备及其应用

用甲醛灭活副溶血弧菌(Vibrio parahaemolyticus)和溶藻弧菌(V.alginolyticus)制成免疫原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术,获得1株特异的针对副溶血弧菌的单克隆抗体,命名为D6F3H5。腹水及培养上清液抗体的ELISA效价分别为:1∶5 120和1∶1 280,该单克隆抗体与其它细菌没有明显的交叉反应。利用该单克隆抗体和兔抗副溶血弧菌多克隆抗体,建立了检测副溶血弧菌的双抗体夹心ELISA方法。该方法对副溶血弧菌的最小检出量为5×104个/mL。用双抗体夹心ELISA检测大黄鱼(Pseudosciaena crocea)样品,14尾患病大黄鱼中有11尾检出副溶血弧菌,而10尾健康大黄鱼都没有检出副溶血弧菌。由此可见,本试验制备的高特异性的抗副溶血弧菌单克隆抗体,可以用于患病大黄鱼副溶血弧菌的快速诊断。     

“Digital earth” in support of an online oceanic educational public service and popularization

According to the initial vision of "digital earth" (DE), the public should constitute a significant proportion of its users. However, to date, most of the studies and applications have focused on science, the private sector and government. A DE-supported online oceanic educational public service and popularization system, iOcean, is studied. First, the vision for the public’s engagement with "digital ocean" is described: an analysis is presented from four aspects, i.e., the space dimension, the time dimension, the state dimension and its relationship with human beings. Second, the technical framework of iOcean is discussed, including data updating and model computing, the data, the function, and the application layers. Third, two key technologies are studied in detail that will enable the construction of iOcean. More than half a million public viewers have used the current version’s website. Practical demonstrations show that iOcean can bring virtual oceans to web browsers and desktops and construct a bridge between government departments and the general public.     

Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.