Base cation concentrations in forest litter and topsoil have different responses to climate and tree species along elevational gradients

The forest litter is an essential reservoir of nutrients in forests, supplying a large part of absorbable base cations(BC) to topsoil, and facilitating plant growth within litter-soil system. To characterize elevational patterns of base cation concentrations in the forest litter and topsoil, and explore the effects of climate and tree species, we measured microclimate and collected the forest litter and topsoil(0-10 cm) samples across an elevational range of more than 2000 m(1243 ～ 3316 m a.s.l.),and analyzed the concentrations of BC in laboratory. Results showed that: 1) litter Ca concentration displayed a hump-shaped pattern along the elevational gradients, but litter K and Mg showed saddle-shaped patterns. Soil Ca concentration increased with elevation, while soil K and Mg had no significant changes. 2) Ca concentration in the forest litter under aspen(Populus davidiana) was significantly higher than that in all other species, but in topsoil, Ca concentration was higher under coniferous larch and fir(Larix chinensis and Abies fargesii). Litter K and Mg concentrations was higher under coniferous larch and fir, whereas there were nosignificant differences among tree species in the concentrations of K and Mg in topsoil. 3) Climatic factors including mean annual temperature(MAT), growing season precipitation(GSP) and non-growing season precipitation(NGSP) determined BC concentrations in the forest litter and topsoil. Soil C/N and C/P also influenced BC cycling between litter and soil. Observation along elevations within different tree species implies that above-ground tree species can redistribute below-ground cations, and this process is profoundly impacted by climate. Litter and soil Ca, K and Mg with different responses to environmental variables depend on their soluble capacity and mobile ability.     

Verification and recalibration of HY-2A microwave radiometer brightness temperature

HY-2A is the first one of the Chinese HY-2 ocean satellite series carrying a microwave radiometer(RM)to measure sea surface temperature,sea surface wind speed,atmospheric water vapor,cloud liquid water content,and rain rate.We verified the RM level 1B brightness temperature(T B)to retrieve environmental parameters.In the verification,TB that simulated using the ocean-atmosphere radiative transfer model(RTM)was used as a reference.The total bias and total standard deviation(SD)of the RM level 1B TB,with reference to the RTM simulation,ranged-20.6-4.38 K and 0.7-2.93 K,respectively.We found that both the total bias and the total SD depend on the frequency and polarization,although the values for ascending and descending passes are different.In addition,substantial seasonal variation of the bias was found at all channels.The verification results indicate the RM has some problems regarding calibration,e.g.,correction of antenna spillover and antenna physical emission,especially for the 18.7-GHz channel.Based on error analyses,a statistical recalibration algorithm was designed and recalibration was performed for the RM level 1B TB.Validation of the recalibrated TB indicated that the quality of the recalibrated RM level 1B TB was improved significantly.The bias of the recalibrated T B at all channels was reduced to<0.4 K,seasonal variation was almost eradicated,and SD was diminished(i.e.,the SD of the 18.7-GHz channel was reduced by more than 0.5K).     

Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

建筑物侧面轮廓线约束的高分辨率遥感影像建筑物高度估算方法

针对现有利用阴影长度法提取建筑物高度时存在的阴影间相互遮挡问题,提出了一种基于建筑物侧面轮廓线进行建筑物高度估算的新方法。首先,利用RPC模型计算建筑物像点位移的方向与卫星成像角度,再将遥感影像进行旋转,使建筑物像点位移沿水平方向;然后,利用Canny算法进行轮廓检测,并构建一定长度的矩形形态学结构元素,对轮廓图像进行形态学开运算,以提取侧面轮廓线,再利用Hough变换与建筑物角点约束,对所提取的轮廓线进一步筛选;最后,根据卫星侧视成像时建筑物高度与像点位移的几何关系进行建筑物的高度估算。利用实际的高分辨率卫星影像对本文方法进行了验证,并与阴影法估算建筑物高度进行了对比。试验结果证明,利用建筑物侧面轮廓线进行建筑物高度估算平均误差可以达到0.7 m,且实际精度优于使用阴影法进行建筑物高度估算。     

无人海洋测绘技术体系构建

随着无人技术的快速发展,无人测绘的时代即将到来。本文分析了无人时代技术特征与海洋测绘发展机遇;讨论了无人海洋测绘新理念,构建了无人海洋测绘技术体系,论述了无人海洋测绘技术关键技术;展望了无人海洋测绘技术发展趋势,及其在海洋经济、海洋可持续发展中的应用前景。     

低空摄影测量立体影像匹配的现状与展望

影像匹配是在两幅或多幅具有重叠度的影像中通过特定的算法提取影像间同名点的过程,是低空摄影测量数据处理中最为关键的步骤,匹配质量与效率直接影响到后续数据处理的成功与否,关系到测绘产品生成质量。本文系统阐述了低空摄影测量影像匹配的研究现状与展望。对影像匹配的分类进行总结和归纳,大体上,影像匹配可划分为两大类,即基于灰度和基于特征的匹配。重点针对基于特征的影像匹配,从点、线、面等特征提取算法及特征描述符和相似性测度与策略等方面进行了详细阐述。此外,列举最新的基于深度学习的影像匹配算法,对低空平台搭载的多样化传感器数据融合可能涉及的影像匹配方法进行了展望。     

VIIRS/DNB夜间灯光月度产品插补方法对比——以北京为例

新一代VIIRS/DNB(Visible Infrared Imaging Radiometer Suite Day/Night Band)夜间灯光数据因其具备更精细的时空分辨率、数据不存在饱和现象、不同年份数据可比性强等优点而迅速代替DMSP/OLS(Defense Meteorological Satellite Program's Operational Linescan System)夜间灯光数据成为新的研究热点。但由于杂散光的污染,VIIRS/DNB夏季数据数值缺失严重,致使数据在空间和时间上不连续,因此,插补缺失数据成为后续应用的前提。鉴于此,论文从插补结果异常值、与参照值对比、计算性能三个方面,系统比较了三次样条插值(样条)、三次Hermite插值(Hermite)、灰色预测模型(GM)、三次指数平滑法(指数)4种插补方法的适用性,以期为插补VIIRS/DNB夜间灯光数据提供方法选择的依据。研究结果表明：①异常值比较方面,Hermite法未出现异常值,另外3种算法仅出现少量异常值(0.02%~1.34%);②与参照值的对比方面,Hermite法与参考值接近程度最高,GM接近程度最低,样条法和指数法介于两者中间;③算法性能比较方面,4种方法都具备计算简单、容易编程的特点,但指数法的算法时长是另外3种方法的10倍以上。因此,综合多方表现,当插补月份前后两侧均有足够长的原始数据时,插补效果好、计算速度快、不会出现过冲现象的Hermite法最适宜,样条法次之;当插补月份仅单侧有足够长的数据时,适宜采用指数法(插补效果好、计算速度较慢)或GM(插补效果偏低、计算速度快)进行插补。     

基于SLAF-seq技术的黑棘鲷微卫星标记开发及其在鲷科鱼类中的通用性研究

黑棘鲷(Acanthopagrusschlegelii)是广泛分布于西北太平洋的暖温性中下层经济鱼类,也是我国沿海重要的海洋捕捞鱼类和增养殖对象。然而,目前有关黑棘鲷的微卫星标记研究报道较少,难以对其种质资源状况作出精确评估。本研究采用SLAF-seq技术测序共获得22489个二至六碱基重复的黑棘鲷微卫星序列,短重复序列(二、三碱基)占总微卫星序列的90.8%,长重复序列(四至六碱基)占有9.2%。经过157对随机合成引物的多态性筛选,开发出49个高多态性的黑棘鲷微卫星标记,其中短重复序列位点有25个,长重复序列位点有24个。每个位点的等位基因数(Na)为2—20(均值为8.3),观测杂合度(Ho)和期望杂合度(He)分别为0.097—0.938和0.122—0.922(均值分别为0.663和0.701),多态信息含量(PIC)为0.118—0.897(均值为0.655)。经Bonferroni校正后,有47个位点符合哈迪-温伯格平衡(HWE),各位点间未检测到连锁不平衡现象,仅2个位点偏离HWE。结果表明,所开发的大部分微卫星标记具有高多态性,蕴含的遗传信息含量较为丰富,能够为黑棘鲷的种群遗传资源评估提供数量充足、类型多样的有效分子标记。跨物种扩增结果显示,有43个黑棘鲷微卫星标记可在9种鲷科鱼类中成功扩增,其中28个标记在太平洋棘鲷(Acanthopagruspacificus)、黄鳍棘鲷(Acanthopagruslatus)和澳洲棘鲷(Acanthopagrusaustralis)中具有较好的通用性,2个标记在平鲷(Rhabdosargussarba)、蓝点赤鲷(Pagruscaeruleostictus)、真赤鲷(Pagrusmajor)、二长棘犁齿鲷(Evynnis cardinalis)及黄牙鲷(Dentex hypselosomus)中具有通用性。这些通用性标记可为阐明鲷科属、种间的系统进化关系和棘鲷属鱼类的群体遗传学分析提供新的标记来源和研究角度。     

基于高通量测序方法分析荣成月湖潮间带趋磁细菌多样性

荣成月湖是一个典型天然潟湖,潮间带沉积物中存在多种形态趋磁细菌。通过Roche454高通量测序平台,对沉积物样品(B_S)和磁收样品(B_M)进行16SrRNA基因高通量测序,认识荣成潮间带沉积物的细菌群落结构,并了解趋磁细菌多样性及系统进化地位。研究结果表明沉积物样品中主要的细菌类群为δ-变形菌纲,占总细菌数的26.4%,其次是γ-变形菌纲和α-变形菌纲;而磁收样品中细菌多样性和种类明显降低,以α-变形菌纲占绝对优势,相对比例达72.6%。在磁收样品和沉积物样品分别发现了1 612条和186条reads与趋磁细菌相关,分别占细菌总数的5.76%和0.85%,磁收样品中趋磁细菌数是沉积物样品的6.8倍。对两个样品中获得的趋磁细菌序列进行系统进化分析,发现这些序列多数属于变形菌门的α-变形菌纲,以趋磁球菌占绝对优势,少数属于δ-变形菌纲,与多细胞趋磁原核生物亲缘关系最近。海洋趋磁螺菌属仅在B_M样品中检出,趋磁弧菌属在B_M样品优势度高于B_S样品,而多细胞趋磁原核生物和趋磁螺菌属在B_S样品中优势度更高。通过分析样品间的差异OTUs,认为荣成潮间带沉积物中可能存在大量未知的趋磁细菌新类群。研究结果为下一步培养和开发趋磁细菌这一功能菌群,发现趋磁细菌新类群及趋磁细菌生态功能提供了基础资料。