Bedrock morphology and structures within overlying sediments,Start Bay,Southwest England,determined by continuous seismic profiling,side-scan sonar,and core sampling

The bedrock morphology of Start Bay, Southwest England, and the Structures within overlying sediments have been determined by a combined geophysical and geological survey. It has been established that the Skerries Bank consists of a drape of possibly coarse, unconsolidated material (bank deposits) overlying medium- to fine-grained silty sand (bay deposits). A break of slope in the bedrock surface, traced along the entire length of the Bay, and a step profile in the southern part of the Bay, may be either a legacy of submerged topography produced by vigorous tidal action, or alternatively the product of subaerial and marine processes. Other significant geological features in the Bay include infilled valleys, and “terraces” possibly pertaining to relict barrier beaches.     

Immunological characterization of metallothionein in marine and freshwater fish

Metallothionein (MT) is a low-molecular-weight, cysteine-rich, metalbinding protein found in all vertebrates. The primary structure of the protein is evolutionary conserved, especially the location of cysteine-residues. There are nonetheless significant differences between MTs from different vertebrate classes, notably in major antigenic epitopes. Previous work has shown that MT from different fish species also differ. The objective of the present work was to characterize MT from temperate, sub-tropical and tropical fish species by their relative reactivities with two anti-fish MT antisera. One or both antisera cross-reacted with MT from all fish species investigated. Surprisingly, the relative reactivities of the two antisera with MT from species belonging to different taxonomic groups appeared to reflect differences at the level of family rather than differences between major phylogenetic lineages.     

Zinc concentration effect at the organismal,cellular and subcellular levels in the eastern oyster

The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc.     

Numerical experiments on the four-dimensional assimilation of the observation data derived in the Black Sea in June 1984 on the basis of a numerical energy-balanced model

A procedure for the four-dimensional (4D) analysis of the hydrophysical fields in the Black Sea with an assimilation of the temperature and salinity (T, S) data was realized on the basis of a numerical model which involves the primitive equations of motion, and the heat and salt advection equations. Two experiments were carried out which differed by the observation data assimilation procedure. Analysis has shown that the observation data assimilation procedure realized using the energy-balanced model allows the reproduction of some synoptic features of the circulation in the Black Sea. A comparison of two computations demonstrates the efficiency of assimilating the measurement data on the basis of the 4D analysis as compared with the sequential objective analysis.Translated by Mikhail M. Trufanov.     

钝顶螺旋藻部分原生质体及单细胞的制备与培养

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。     

Nitrate assimilation by zooxanthellae maintained in laboratory culture

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.     

A general method for calculating three-dimensional laminar and turbulent boundary layers on ship hulls

A general method for representing the flow properties in the three-dimensional boundary layers around ship hulls of arbitrary shape is described. It makes use of an efficient two-point finite-diffirence schem to solve the boundary-layer equations and includes an algebraic eddy-viscosity representaion of the Reynolds-stress ternsor. The numericzal method contains novel and desirable features and allows the calculation of flows in which the circumferential velocity component contains regions of flow reversal across the boundary layer. The inviscid pressure distribution is determined with the Douglas-Neumann method which, if necessary, can conveniently allow for the boundary-layer displacement surface. To allow its application to ships, and particularly to those with double-elliptic and flat-bottomed hulls, a non-orthogonal coordinate system has been developed and is shown to be economical, precise and comparatively easy to use. Present calculations relate to zero Froude number but they can be extended to include the effects of a water wave and local regions of flow separation which may stem from bulbous-bow geometries.     

Diel fluctuations in dissolved free amino acids and monosaccharides in Chesapeake Bay dinoflagellate blooms

The accumulation of phytoplankton biomass in recurring summer dinoflagellate blooms of Chesapeake Bay is accompanied by large pools of dissolved organic matter (DOM). Two fractions of the DOM, free amino acids (DFAA) and monosaccharides (MONO), were measured at 3 h intervals in mixed species dinoflagellate blooms (Katodinium rotundatum, Gymnodinium spp.) and related to productivity, biomass and photoperiod. Peak chlorophyll levels for the three blooms were 28, 65 and 938 μg1⁻¹. In general, DFAA and MONO concentrations increased with increasing biomass of bloom-forming species, reaching 203 and 844 μg1⁻¹. MONO appeared to accumulate during the day while there was no consistent pattern for DFAA. The accumulations of DFAA and MONO in blooms indicate that bloom production might stimulate microheterotrophy, thereby enhancing carbon and nutrient cycling in bloom-impacted regions.