Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

连续暴雨过程中的中-β尺度大暴雨的成因分析

利用常规资料、多普勒雷达等资料，对威海市2004年8月4日夜间到6日早上连续3天暴雨大暴雨降水过程进行了分析，重点分析了5日夜间的中-β尺度大暴雨过程，发现此次降水是发生在副高边缘的高能量堆积区，冷空气的侵入是前期强降水的主要原因，而地面辐合是导致强降水再次出现的主要触发机制，充沛的水汽输送、聚集的对流有效能量为暴雨中尺度系统发生发展提供了必要的物理机制。     

Constraint on Parameters of Inverse Compton Scattering Model for PSR B2319+60

Using the multifrequency radio profiles of pulsar PSR B2319+60, two parameters of inverse Compton scattering model, the initial Lorentz factor and the factor of energy loss of relativistic particles are constrained.     

太原地震台水位异常分析

针对太原地震台晋7-1井近年来水位大幅上升情况,进行资料收集和干扰因素分析,结果发现：2009年7月以来的井水位回升,与关闭小煤窑、汾河清水复流工程、关井压采、大量引用黄河水有直接关系。     

松花江水下爆破孔施工技术

在东北严寒季节,在松花江上施工水下爆破孔。介绍了施工设备及施工平台,根据不同地层分别采用静压振动下套管护壁及潜孔锤钻进方法,设计了风路系统,改进了套管妆方法,并采取了科学的管理方法,取产好的效果。     

重庆市巴南区地热资源开发利用现状及前景分析

巴南区地热资源丰富,热储总存量为3.105×1015KJ,热流体储存总量为6.855×108m3。经30多年的开发利用,已初步形成了以东温泉、南温泉、桥口坝温泉为核心的温泉旅游开发格局,主要用于温泉房地产开发、风景区、温泉旅游、康乐疗养休闲、商务会展等。为提升开发利用水平,巴南区地热水资源开发要以市场为导向,以效益为目标,突出重点、打造精品、强化特色、完善配套,建设特色温泉产业。     

九江-瑞昌5.7级地震余震的S波分裂研究

利用在九江-瑞昌MS5.7地震震中附近架设的丁家山台(DJS)、狮子洞台(SZD)和武蛟台(WUJ)的地震波形资料,采用S波分裂系统分析方法,对余震进行了S波分裂分析。结果表明,震中距较小、台站附近断层分布复杂的丁家山台(DJS)的慢波时间延迟相对较大,快波偏振方向不太集中。台站附近断裂分布单一的武蛟台(WUJ)快波偏振优势方向与断裂走向角度相差约35°,与区域主压应力方向也不一致。台站附近无断层通过的狮子洞台(SZD)快波偏振优势方向接近区域主压应力方向。慢波时间延迟大小与震源深度没有明显的规律性关系。     

Excessive greenhouse gas emissions from wastewater treatment plants by using the chemical oxygen demand standard

Chemical oxygen demand(COD)is widely used as an organic pollution indicator in wastewater treatment plants.Large amounts of organic matter are removed during treatment processes to meet environmental standards,and consequently,substantial greenhouse gases(GHGs)such as methane(CH₄)are released.However,the COD indicator covers a great amount of refractory organic matter that is not a pollutant and could be a potential carbon sink.Here,we collected and analysed COD data from 86 worldwide municipal wastewater treatment plants(WWTPs)and applied a model published by the Intergovernmental Panel on Climate Change to estimate the emission of CH₄ due to recalcitrant organic compound processing in China’s municipal wastewater treatment systems.Our results showed that the average contribution of refractory COD to total COD removal was55%in 86 WWTPs.The amount of CH₄ released from the treatment of recalcitrant organic matter in 2018 could have been as high as 38.22 million tons of carbon dioxide equivalent,which amounts to the annual carbon sequestered by China’s wetlands.This suggests that the use of COD as an indicator for organic pollution is undue and needs to be revised to reduce the emission of GHG.In fact,leaving nontoxic recalcitrant organic matter in the wastewater may create a significant carbon sink and will save energy during the treatment process,aiming at carbon neutrality in the wastewater treatment industry.