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91.
Yingchun Lv Rong Zheng Tao Zuo Yuming Wang Zhaojie Li Yong Xue Changhu Xue Qingjuan Tang 《中国海洋大学学报(英文版)》2014,13(5):825-829
Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible. 相似文献
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Using the multifrequency radio profiles of pulsar PSR B2319+60, two parameters of inverse Compton scattering model, the initial Lorentz factor and the factor of energy loss of relativistic particles are constrained. 相似文献
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利用在九江-瑞昌MS5.7地震震中附近架设的丁家山台(DJS)、狮子洞台(SZD)和武蛟台(WUJ)的地震波形资料,采用S波分裂系统分析方法,对余震进行了S波分裂分析。结果表明,震中距较小、台站附近断层分布复杂的丁家山台(DJS)的慢波时间延迟相对较大,快波偏振方向不太集中。台站附近断裂分布单一的武蛟台(WUJ)快波偏振优势方向与断裂走向角度相差约35°,与区域主压应力方向也不一致。台站附近无断层通过的狮子洞台(SZD)快波偏振优势方向接近区域主压应力方向。慢波时间延迟大小与震源深度没有明显的规律性关系。 相似文献
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Zongqing LV Xiaoyu SHAN Xilin XIAO Ruanhong CAI Yao ZHANG Nianzhi JIAO 《中国科学:地球科学(英文版)》2022,65(1):87-95
Chemical oxygen demand(COD)is widely used as an organic pollution indicator in wastewater treatment plants.Large amounts of organic matter are removed during treatment processes to meet environmental standards,and consequently,substantial greenhouse gases(GHGs)such as methane(CH4)are released.However,the COD indicator covers a great amount of refractory organic matter that is not a pollutant and could be a potential carbon sink.Here,we collected and analysed COD data from 86 worldwide municipal wastewater treatment plants(WWTPs)and applied a model published by the Intergovernmental Panel on Climate Change to estimate the emission of CH4 due to recalcitrant organic compound processing in China’s municipal wastewater treatment systems.Our results showed that the average contribution of refractory COD to total COD removal was55%in 86 WWTPs.The amount of CH4 released from the treatment of recalcitrant organic matter in 2018 could have been as high as 38.22 million tons of carbon dioxide equivalent,which amounts to the annual carbon sequestered by China’s wetlands.This suggests that the use of COD as an indicator for organic pollution is undue and needs to be revised to reduce the emission of GHG.In fact,leaving nontoxic recalcitrant organic matter in the wastewater may create a significant carbon sink and will save energy during the treatment process,aiming at carbon neutrality in the wastewater treatment industry. 相似文献
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