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Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study, we introduced a Vitreoscilla hemoglobin gene (vgb) into Chlorella vulgaris by Agrobacterium tumefaciens-mediated transformation (ATMT). PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L, which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants, which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris, with applications to lutein production from Chlorella during fermentation. 相似文献
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采用RT-PCR技术, 从雨生红球藻(Haematococcus pluvialis)中克隆出IPP异构酶基因(ipiHp1), 构建双元载体pCAMBIA3300-egfp-ipiHp1和基因枪转化载体pBlueScript SKⅡ-bar-egfp-ipiHp1, 利用根癌农杆菌侵染法和基因枪转化法, 将目的基因导入雨生红球藻中, 经过含50?g/mL草丁膦BBM培养基筛选得到阳性转化子。通过荧光观察报告基因egfp和PCR扩增分析, 证明ipiHp1基因整合到转化子的基因组中。生物量测定结果表明大部分转化子的生物量与野生型相似。虾青素含量测定发现, 农杆菌侵染法转化的转化子A3虾青素含量与野生型相比有显著变化, 平均值达到16.49mg/g, 与野生型相比提高了5.16%, 而基因枪转化法转化的转化子虾青素含量与野生型无显著性差异。 相似文献
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真空冷冻干燥微藻DHA油纳米乳工艺研究 总被引:1,自引:0,他引:1
采用超声波法制备微藻DHA油纳米乳,研究了微藻DHA油纳米乳真空冷冻干燥工艺,并对其粉末进行了表征。研究结果表明,当冻干曲线为:预冻:–40℃维持2 h,真空度为0.2 mbar;升华干燥:–30℃维持12 h,真空度为0.2 mbar,–20℃维持2.5 h,真空度为0.15 mbar;解析干燥:25℃维持3 h,真空度为0.15 mbar,且油相与冻干保护剂的质量比为1:4,冻干保护剂选择葡萄糖:甘露醇质量比为1:3时,制备的纳米乳粉末颗粒外观呈乳白色、均匀细腻,复溶速度在10s内,冻干前粒径为(175.6±22.1)nm,多分散系数(PDI)为(0.15±0.62),冻干后粒径为(211.1±23.5)nm,PDI为(0.152±0.116),差异不大,依旧在纳米范围内,不仅保证了DHA的长期稳定性,还扩大了DHA的应用范围,有望成为DHA的新型纳米载药系统。 相似文献
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