全文获取类型
收费全文 | 111篇 |
免费 | 3篇 |
国内免费 | 24篇 |
专业分类
海洋学 | 119篇 |
综合类 | 18篇 |
自然地理 | 1篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 10篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 2篇 |
2011年 | 2篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 3篇 |
2007年 | 10篇 |
2006年 | 10篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 10篇 |
2002年 | 10篇 |
2001年 | 4篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
排序方式: 共有138条查询结果,搜索用时 15 毫秒
61.
62.
本文以青岛大扁藻 (Platymonassubcordiformis) ,球等鞭金藻 870 1 (Isochrysis galbana870 1 )为试验材料 ,采用水生毒理联合效应指数法 ,比较了 3种多环芳烃 ,蒽 (anthracene) ,并 [a]芘 (benzo[a] pyrene)及 1 ,2 -苯并蒽 (1 ,2 - benzonthracene)单剂和混剂对青岛大扁藻和球等鞭金藻 870 1的急性毒性和联合毒性。实验结果表明 :对青岛大扁藻 ,胁迫 72 h,蒽与苯并 [a]芘的联合及苯并 [a]芘与 1 ,2 -苯并蒽的联合为协同作用 ,蒽与 1 ,2 -苯并蒽的联合及蒽 ,苯并 [a]芘与 1 ,2 -苯并蒽的联合为拮抗作用。对球等鞭金藻 870 1胁迫 72 h,苯并 [a]芘与 1 ,2 -苯并蒽的联合为协同作用 ,其它联合均为拮抗作用。 相似文献
63.
通过计算机图像测定系统测定、计算获得海冰细菌的大小、面积和碳含量,研究了北极巴伦支海和拉普捷夫海的浮冰中细菌大小、丰度和生物量的垂直分布及其与海冰微藻生物量的关系.细菌在冰心中是非均匀分布的,至少有两种不同分布类型:第一类有单一的丰度峰值,在冰心底部或在冰心中部;第二类有两个丰度峰值.海冰中细菌的丰度为0.4×105~36.7×105个/cm3.不同类型海冰中的细菌大小变化极大,在多年冰中,最大的细菌与叶绿素a最大值出现在同一层,而在一年冰中,细菌大小几乎没有垂直变化.整柱冰心的细菌生物量变化为19.2~79.2mg/cm2,细菌与海冰微藻生物量之比为0.43~10.00.对固定冰比较和研究的结果发现,海冰冰心中细菌大小、丰度和生物量的垂直分布差别极大.据此,分析了对目前海冰研究采样方法的局限性,并提出了规范采样方法的设想. 相似文献
64.
多环芳烃对3种赤潮微藻类胡萝卜素含量的影响及其对UV-B辐射的响应 总被引:1,自引:0,他引:1
评价UV-B辐射增强对海洋生态系统的影响,通过实验生态学的方法,研究3种多环芳烃——菲、芘和蒽对3种赤潮微藻——赤潮异弯藻、塔玛亚历山大藻和中肋骨条藻类胡萝卜素含量的影响。结果表明:低浓度的菲、芘和蒽处理使3种赤潮微藻的类胡萝卜素含量有所提高,而高浓度处理则降低藻细胞内的类胡萝卜素含量。菲、芘和蒽处理对赤潮异弯藻类胡萝卜素含量的96 h半抑制剂量分别为0.098,0.124和0.139 mg.L-1,对中肋骨条藻的96 h半抑制剂量分别为0.120,0.147和0.155 mg.L-1,对塔玛亚历山大藻的96 h半抑制剂量分别为0.128,0.153和0.162 mg.L-1。在菲、芘和蒽处理的同时,附加辐射剂量为0.3 J.m-2的UV-B辐射处理,3种赤潮微藻的类胡萝卜素含量显著降低。 相似文献
65.
采用植物单细胞和原生质体游离的方法,研究了5株褐藻酸降解菌感染过程中海带细胞壁的组成成分——褐藻酸的变化。结果表明,海带在5株褐藻酸降解菌感染的初期(前3 d),褐藻酸酶对其单细胞和原生质体的游离率均显著降低,而且随着感染时间的延长,游离率的下降越加明显。3 d过后,随着感染的继续进行,单细胞和原生质体的游离率变化不再明显。表明海带细胞壁组成对褐藻酸降解菌感染发生了响应性变化,而这种变化仅仅发生在褐藻酸降解菌感染的早期阶段。研究结果进一步显示,褐藻酸降解菌感染过程中海带细胞壁组成的变化主要体现在褐藻酸的变化上。 相似文献
66.
Oxygen and carbon isotope ratios(δ~(18)O and δ~(13)C) in otoliths were used to identify the stock structure of small yellow croaker,Larimichthys polyactis.Otoliths were collected from fish at five locations across the Yellow Sea and the Bohai Sea representing most of their distributional range and fisheries areas.The significant differences in the isotopic signatures showed that the five locations could be chemically distinguished and clearly separated,indicating stock subdivision.Correlation of δ~(18)O and δ~(13)C values suggested that population of L.polyactis could be divided into the Bohai Sea group,the southern Yellow Sea group and the central Yellow Sea group.Discriminant analysis of δ~(18)O and δ~(13)C values demonstrated a high significant difference with 85.7% classification accuracy.The spatial separation of L.polyactis indicated a complex stock structure across the Yellow Sea and the Bohai Sea.These results indicate that optimal fisheries management may require a comprehensive consideration on the current spatial arrangements.This study has provided further evidence that measurement of the stable isotopes ratios in otolith can be a valuable tool in the delineation of fishery management units. 相似文献
67.
Members of the DnaJ family are proteins that play a pivotal role in various cellular processes, such as protein folding, protein transport and cellular responses to stress. In the present study, we identified and characterized the full-length DnaJ cDNA sequence from expressed sequence tags of Pyropia yezoensis (PyDnaJ) via rapid identification of cDNA ends. This cDNA encoded a protein of 429 amino acids, which shared high sequence similarity with other identified DnaJ proteins, such as a heat shock protein 40/DnaJ from Pyropia haitanensis. The relative mRNA expression level of PyDnaJ was investigated using real-time PCR to determine its specific expression during the algal life cycle and during desiccation. The relative mRNA expression level in sporophytes was higher than that in gametophytes and significantly increased during the whole desiccation process. These results indicate that PyDnaJ is an authentic member of the DnaJ family in plants and red algae and might play a pivotal role in mitigating damage to P. yezoensis during desiccation. 相似文献
68.
Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella salina in laboratory cultures based on diff erent temperature (15°C, 20°C, and 25°C) and lighting (40, 80, and 160 μmol/(m2·s)) conditions. The growth of D. salina in bi-algae culture (1:1 size/density) was significantly restrained. The results of cell-free filtrate culture indicate that direct cell-tocell contact was not necessary in interspecific competition. Further experimental results demonstrated that allelochemicals released from K. mikimotoi were markedly influenced by both temperature (P =0.013) and irradiance (P =0.003), resulting in diff erent growth characteristics of D. salina in filtrate mediums. Compared with the plateau period, K. mikimotoi exudates in the exponential phase had a stronger short-term inhibition effect on D. salina in normal conditions. A clear concentration-dependent relationship was observed in the effect of allelochemicals released from K. mikimotoi with low-promoting and high-repressing effects on D. Salina in a short time-scale. In addition, allelopathic substances remain stable and effective under high temperature and pressure stress. Many flocculent sediments adhering with D. salina cells were observed in all filtrate mediums, while the quantity and color depended on the original culture conditions. 相似文献
69.
通过逐级提高氯化钠浓度的筛选方法获得了小麦耐盐细胞系。与对照细胞系相比 ,耐盐细胞系在盐分胁迫下能维持较高的超氧化物歧化酶、过氧化物酶和过氧化氢酶活性 ,同时具有较强的脂溶性抗氧化能力。说明在细胞水平上小麦对盐分耐受力的高低与其抗氧化能力的强弱密切相关。 相似文献
70.
Possible mediatory roles of heat acclimation and salicylic acid in protecting the sporophyte of marine macroalga Laminaria japonica (Phaeophyceae) from heat stress were studied. Heat stress resulted in oxidative injury in the kelp blades. Under heat stress
significant accumulation of hydrogen peroxide (H2O2) and malonaldehyde (MDA), a membrane lipid peroxidation product, and a drastic decrease in chlorophyll a content were recorded. Activity of the enzymatic antioxidant system was drastically affected by heat stress. The activity
of superoxide dismutase (SOD) was significantly increased while peroxidase (POD), catalase (CAT) and glutathione peroxidase
(GPX) were greatly inhibited and, simultaneously, phenylalanine ammonia-lyase was activated while polyphenol oxidase (PPO)
was inhibited. Both heat acclimation pretreatment and exogenous application of salicylic acid alleviated oxidative damage
in kelp blades. Blades receiving heat acclimation pretreatment and exogenous salicylic acid prior to heat stress exhibited
a reduced increase in H2O2 and MDA content, and a lower reduction in chlorophyll a content. Pretreatment with heat acclimation and salicylic acid elevated activities of SOD, POD, CAT, GPX and PPO. Considering
these results collectively, we speculate that the inhibition of antioxidant enzymes is a possible cause of the heat-stress-induced
oxidative stress in L. japonica, and enhanced thermotolerance may be associated, at least in part, with the elevated activity of the enzymatic antioxidant
system. 相似文献