中国珊瑚礁资源状况及其药用研究调查 Ⅱ.资源衰退状况、保护与管理

对中国珊瑚礁资源衰退状况和原因进行了调查和分析.结果表明,中国南海珊瑚礁资源衰退状况严重,珊瑚礁破坏率高达 90%以上,其中,占全国珊瑚礁总面积98%的海南,80%～95%的珊瑚礁受到破坏.除自然因素外,对珊瑚礁资源的不当的、过度的开发利用,社会经济发展带来的海洋环境污染等人为因素,是珊瑚礁资源衰退的主要原因.建立南中国海珊瑚礁生态系统保护与管理国际合作机制,正确评估珊瑚礁的生态功能与价值,建立珊瑚礁自然保护区及监测网络系统,是中国珊瑚礁资源保护性开发利用的可行对策.     

中国珊瑚礁资源状况及其药用研究调查 Ⅲ.民间药用与药物研究状况

对中国珊瑚药用状况和研究现状进行了调查.结果表明,中国有造礁石珊瑚、柳珊瑚、软珊瑚等珊瑚物种 490 种,民间药用的仅有22种.目前,已经对100种南海软珊瑚和柳珊瑚进行了化学成分的研究.其中,软珊瑚以豆荚软珊瑚Lobophytum,肉芝软珊瑚Sarcophyton,短指软珊瑚Sinularia,柔荑软珊瑚Nephthea和异花软珊瑚Xenia等属研究较多,柳珊瑚以小月柳珊瑚Menella,竹节柳珊瑚Isis和灯芯柳珊瑚Junceella等属研究较多.主要化合物结构类型包括二萜、甾体、倍半萜,含氮化合物等,其中部分化合物显示较强的抗肿瘤、抗菌、抗氧化、防生物附着等多种生物活性,具有潜在的药物开发应用价值.     

Diagnostic model construction and example analysis of habitat degradation in enclosed bay: III. Sansha Bay habitat restoration strategy

Unbalanced inputs and outputs of material are the root cause of habitat degradation in Sansha Bay,Fujian Province,China. However,the cumulative pollution varies in different geographic locations and natural conditions in the enclosed bay. In this study,hydrodynamic conditions,sediment characteristics,and aquaculture methods were recognized as the underlying causes of spatial heterogeneity in the distribution of nitrogen and phosphorous pollutants,the two major controlling factors of habitat degradation in the bay area. In order to achieve the goal of balancing nutrient inputs and outputs in Sansha Bay,we developed a feasible and practical zone restoration strategy for reasonable adjustment and arrangement of aquaculture species and production scale in accordance with varying hydrodynamic conditions and sediment characteristics in six sub-bay areas(sub-systems). The proposed zone restoration strategy lays a solid foundation for habitat restoration and management in Sansha Bay.     

Characterization and analysis of ribosomal proteins in two marine calanoid copepods

Copepods are among the most abundant and successful metazoans in the marine ecosystem. However, genomic resources related to fundamental cellular processes are still limited in this particular group of crustaceans. Ribosomal proteins are the building blocks of ribosomes, the primary site for protein synthesis. In this study, we characterized and analyzed the c DNAs of cytoplasmic ribosomal proteins(c RPs) of two calanoid copepods, P seudodiaptomus poplesia and A cartia pacifi ca. We obtained 79 c RP c DNAs from P. poplesia and 67 from A. pacifi ca by c DNA library construction/sequencing and rapid amplifi cation of c DNA ends. Analysis of the nucleic acid composition showed that the copepod c RP-encoding genes had higher GC content in the protein-coding regions(CDSs) than in the untranslated regions(UTRs), and single nucleotide repeats(3 repeats) were common, with "A" repeats being the most frequent, especially in the CDSs. The 3′-UTRs of the c RP genes were signifi cantly longer than the 5′-UTRs. Codon usage analysis showed that the third positions of the codons were dominated by C or G. The deduced amino acid sequences of the c RPs contained high proportions of positively charged residues and had high p I values. This is the fi rst report of a complete set of c RP-encoding genes from copepods. Our results shed light on the characteristics of c RPs in copepods, and provide fundamental data for further studies of protein synthesis in copepods. The copepod c RP information revealed in this study indicates that additional comparisons and analysis should be performed on different taxonomic categories such as orders and families.     

桃花水母的核糖体RNA基因ITS区序列分析及物种鉴定

利用PCR扩增和DNA测序技术,获得了青岛野外采集到的桃花水母(Craspedacusta)核糖体RNA基因ITS区序列。序列比对以及系统进化分析显示,在青岛采集到的桃花水母与索氏桃花水母(Craspedacusta sowerbyi)的ITS区序列相似度最高,同源性在93%以上;同时,在系统发育树中也与索氏桃花水母聚为一支。研究结果表明,青岛采集到的桃花水母为索氏桃花水母,这也是青岛地区桃花水母的新记录。     

2009年夏季黄河口及其邻近水域网采浮游植物的群落结构

为了研究黄河调水调沙对黄河口及其邻近水域浮游植物群落的影响,于2009年7月黄河第九次调水调沙后对黄河口及其邻近水域的浮游植物进行了调查。利用网采浮游植物样品研究了该水域浮游植物的种类组成、丰度分布、优势种以及生物多样性,结果表明调查水域共鉴定出浮游植物29属53种,隶属于硅藻门、甲藻门、蓝藻门、金藻门和绿藻门,其中硅藻43种,甲藻6种,还有少数浮游植物属于蓝藻门、金藻门和绿藻门;浮游植物细胞丰度为0.96×104~76.32×104个/m3,平均丰度为21.23×104个/m3;主要优势种类有念珠藻(Nostocaceae)、八幅辐环藻(Actinocyclus octonarius)、佛氏海线藻(Thalassionema frauenfeldii)和卡氏角毛藻(Chaetoceros castracanei)等;调水调沙引起的黄河径流量的增加对黄河口及其邻近水域浮游植物群落结构的影响显著。该研究揭示了特定时期黄河口及其邻近水域浮游植物的群落结构,并丰富了该水域浮游植物群落的基础资料,为深入探讨河口环境变化对浮游植物群落的影响提供了重要依据。     

三峡工程一期蓄水后长江口及其邻近水域浮游动物的群落结构

长江三峡工程自2003年6月1日~10日进行一期蓄水。为研究长江三峡工程蓄水对长江口环境的影响,在一期蓄水后组织了长江口及其邻近海域环境的综合调查。根据采集的大中型和中小型浮游生物样品的分析数据,结合环境参数,对长江河口及邻近海域浮游动物种类组成及群落结构进行了多元分析,研究浮游动物群落结构及其影响因子。浮游动物种类数为289个(含31种浮游幼虫)。据各站位浮游动物种类组成和丰度,用聚类分析法可将该水域的浮游动物分为4个群落。整个调查海区,中华哲水蚤最占优势。相关性分析表明,夏季影响浮游动物群落结构的主要环境因子为盐度。