文蛤抗癌多肽对N-乙酰-β-D氨基葡萄糖苷酶活力的影响

从文蛤体中分离纯化得到了抗癌多肽,研究该多肽对N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活力的影响,结果显示文蛤抗癌多肽对该酶有明显的抑制作用.在本文中,我们将深入地研究其抑制作用机理和抑制效应类型,结果表明:随着文蛤多肽加入量的增大,该酶活力呈指数下降,测得导致酶活力下降50%的抑制剂浓度(IC50)为112.9 μg/cm3,该多肽对酶的抑制作用是一种可逆过程,抑制机理表现为混合型抑制类型,对游离酶(E)的抑制常数(KI)和酶-底物络合物的抑制常数(KIS)分别为77.03和697.44μg/cm3,说明文蛤抗癌多肽与游离酶的结合导致酶活力的丧失,明显的强于对酶-底物络合物的抑制效应,底物的存在对该酶被文蛤抗癌多肽抑制作用起了保护作用.     

实施软资源整合以提升浙江港口的竞争力

浙江港口群作为上海国际航运中心的重要组成部分,如何根据自身现有条件,加快港口软资源整合,充分发挥港口整体优势以提升港口的竞争力,已成为新时期港口建设的一个重要内容。通过对浙江港口资源现状的分析,从管理体制与经营机制两方面对提升浙江港口竞争力的实施途径进行了论述,提出了浙江港口资源整合和经营机制整合的具体内容以及提升浙江港口竟争力的具体措施。     

井下双向流动阀防漏失技术在埕岛油田的应用

埕岛油田位于渤海西南部极浅一浅海地区,是一个大型潜山披覆构造油田。随着埕岛油田开采力度加强,地层亏空十分严重,作业过程中的地层漏失是困绕埕岛油田油层保护的一大难题。据此介绍了胜利油田海上埕岛油田油层保护的一项新技术——井下双向流动阀防漏失技术的应用情况,阐述了井下双向流动阀的结构、原理以及应用后对油层保护所起到的作用。     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

浅谈信息化测绘在土地利用与管理中的应用

当今社会,土地的利用与管理工作与人们的日常生活密切相关,小到居民住房的购买,大到国家的土地利用总体规划,其工作成效直接影响着人民生活的安居乐业和国家的长治久安。随着测绘相关技术的日臻完善,我国的测绘体系经历了由传统测绘向数字化测绘的转变,目前正在向信息化测绘过渡。信息化测绘是测绘行业发展的必然趋势,其高效的作业模式必然会给土地的利用和管理提供巨大的便利。首先介绍了信息化测绘的内涵及技术体系,然后探讨了信息化测绘技术在土地利用和管理中的应用,最后对信息化测绘技术在土地利用与管理上的发展前景进行了展望。     

测绘生产计划信息一体化管理的解决方案

分析了测绘生产计划制定与管理中所涉及的各类信息的特点及相互关系,提出了一种简单实用的空间数据与属性数据一体化管理的解决方案.通过建立空间数据表和编制空间数据引擎模块,解决了测绘计划存储、下达、上报和管理过程中相关数据的安全性问题;在SQL Anywhere关系数据库中实现了空间数据与属性数据一体化管理.该方案成功应用于测绘生产计划管理系统中.     

Disease resistance and humoral immunomodulatory effects of vitamin C on grouper,Epinephelus awoara

The disease resistance and humoral immunomodulatory effects of vitamin C administered orally to grouper,Epinephelus awoara maintained on a frozen fish diet supplemented with vitamin C at 500, 1000, 1500 and 2000 mg/kg were investigated. After 20 weeks, the growth rates of the groups with high level of vitamin C apparently increased. The untreated fish had symptoms of vitamin C deficiency. The endogenous liver tissue vitamin C levels were found to reflect well the dietary treatments. After intraperitoneal injection or bath challenge with a virulent strain ofVibrio vulnificus, fish fed with high level vitamin C showed significantly higher survival rate compared with the normal control group. Vaccination with formalin inactivatedV. vulnificus significantly enhanced the specific antibody production in fish treated with vitamin C, and completely protected from strong bacterial challenge the groups fed on fish with vitamin C 1500 and 2000 mg/kg diet. Project 39500113 supported by NSFC and also by Guangdong Science Research Foundation (Grant No. 950711).     

Post-depositional modification of NO-3 in snow layers at EastAntarctica and at the headwater of ürumqi River

Acidic species, such as Nitrate, in polar snow and firn layers are “reversibly” deposited, and are sufficiently volatile to undergo significant postdepositional exchange between snow/firn and the atmosphere. Through comparison of the snowpit and snowpack nitrate concentrations from central East Antarctica and the headwater of ürumqi River, we conclude that the nitrate peaks in the uppermost surface snow layers in central Antarctica are not related to an atmospheric signal and must account for post-depositional effects. Such effects, however, are not found in the surface snowpack nitrate profiles from the headwater of ürumqi River. Two reasons may account for the post-depositional difference. At first, nitrate in the polar snow and firn layers appears to be hydrated ion, which can be taken up by the atmosphere, while at the headwater of ürumqi River it seems mainly as mineral ion, which assembles the behavior of aerosol-derived species that are “irreversibly” deposited and do not undergo significant post-depositional exchange with the atmosphere. Secondly, the chemical features of the snow and ice on the Antarctica are mainly determined by wet deposition, to the contrary, dry deposition is more significant at the headwater of lUrumqi River than that on the East Antarctic Plateau.     

Fresh snow chemistry from high mountain regions in central himalayas

Investigations of atmospheric composition in the Himalayas has been limited in both temporal and spatial scales, mainly due to difficult logistics. Ideal sites for monitoring atmospheric composition and its evolution should be free from local pollution and representative of the remote troposphere (HUEBERT et al., 1980). As the Himalayas are far removed from highly industrialized regions they provide suitable locations to monitor the chemistry of the remote troposphere and to study the evolu…     

广东沿海牡蛎体Ni含量水平及其时空变化趋势

198 9～ 1997年间 ,为实施“南海贻贝观察”计划 ,对广东沿海 12个地点近江牡蛎体内的 Ni含量水平进行了连续监测 ,结果全部牡蛎样品均检出 Ni,含量范围为 0 .17～ 2 .37mg/kg(湿重 ) ,总平均值为0 .58mg/kg。污染评价结果显示 ,广东沿海 Ni含量总体上处于微污染—轻污染水平。牡蛎体 Ni平均含量呈珠江口海区 >粤西海区 >粤东海区的分布格局 ,其最高值出现在深圳湾、唐家湾和湛江港。 70年代后期至 1991年 ,牡蛎体的 Ni含量呈明显下降趋势 ,此后 Ni含量年际间虽有波动 ,但无显著差异