Carbon isotopic fractionation in lipids from methanotrophic bacteria: relevance for interpretation of the geochemical record of biomarkers

Experiments with cultured aerobic methane oxidising bacteria confirm that their biomarker lipids will be significantly depleted in 13C compared to the substrate. The methanotrophic bacteria Methylococcus capsulatus and Methylomonas methanica, grown on methane and using the RuMP cycle for carbon assimilation, show maximum 13C fractionation of approximately 30% in the resultant biomass. In M. capsulatus, the maximum fractionation is observed in the earliest part of the exponential growth stage and decreases to approximately 16% as cells approach stationary phase. This change may be associated with a shift from the particulate form to the soluble form of the methane monooxygenase enzyme. Less than maximum fractionation is observed when cells are grown with reduced methane availability. Biomass of M. capsulatus grown on methanol was depleted by 9% compared to the substrate. Additional strong 13C fractionation takes place during polyisoprenoid biosynthesis in methanotrophs. The delta 13C values of individual hopanoid and steroid biomarkers produced by these organisms were as much as l0% more negative than total biomass. In individual cultures, squalene was 13C-enriched by as much as 14% compared to the triterpane skeleton of bacteriohopaneaminopentol. Much of the isotopic dispersion in lipid metabolites could be attributed to shifts in their relative abundances, combined with an overall reduction in fractionation during the growth cycle. In cells grown on methanol, where there was no apparent effect of growth stage on overall fractionation there were still significant isotopic differences between closely related lipids including a 5.3% difference between the hopane and 3 beta-methylhopane skeletons. Hopane and sterane polyisoprenoids were also 13C-depleted compared to fatty acids. These observations have significant implications for the interpretation of specific compound isotopic signatures now being measured for hydrocarbons and other lipids present in sediments and petroleum. In particular, biomarker lipids produced by a single organism do not necessarily have the same carbon isotopic composition.     

Partitioning of silver,beryllium and molybdenum among chemical fractions in the sediments from the Labe (Elbe) river in Central Bohemia,Czech Rep.

Sequential extraction of the fine fraction (63 µm) of the bottom sediments of the Labe river in Central Bohemia indicates that the readily reducible sediment fraction is a major reservoir of silver for release into the aqueous phase. Molybdenum is preferentially mobilized by ion-exchange, while beryllium is accumulated in all the chemical fractions of the sediments, especially in the moderately reducible and the organic/sulphidic phases.Contamination of the aquatic system from anthropogenic sources is characterized by the formation of relatively unstable chemical forms and only a small portion of these elements remain in the stable residual fraction.     

Propagation and absorption of cyclotron maser radiation in solar microwave spike bursts

It has been argued that the loss-cone-driven electron cyclotron maser instability can account for the properties of millisecond microwave spike bursts observed during some solar flares. However, as it propagates outward from the corona, maser radiation undergoes gyroresonance absorption when its frequency is a harmonic of the local electron-cyclotron frequency. Existing analytical models using slab geometries predict that this absorption should be sufficiently strong to prevent the radiation from being seen at the observed levels, except under highly restrictive conditions or for unrealistic plasma parameters. A more comprehensive analysis is presented here to determine if and when maser radiation can escape to produce microwave spike bursts. This analysis employs numerical raytracing and incorporates propagation and absorption of fundamental maser emission in a realistic model of a coronal flux loop. It is found that ranges of physical conditions do exist under which maser radiation can escape to an observer and that these conditions are much more limiting for fundamental emission in the extraordinary ()-mode than in the ordinary (o)-mode. Detailed investigation implies that escaping radiation in the -mode is highly directional and chiefly observable toward the center of the solar disk, while escapingo-mode radiation is found to emerge from the corona over a much wider range of directions, with some cases corresponding to radiation observable near the solar limb.     

The effect of an optically thin region on stability of an accretion disk around a supermassive black hole

We analyse conditions of the innermost portion of an accretion disk and establish a set of equations for this region. A stable innermost region may exist, which can probably explain the observed UV and X-ray spectra, avoiding the unstable emission. We then discuss the detailed radial structure of a disk around a black hole for typical AGN parameters and obtain different kinds of- relationships for different regions of a disk. On the basis of this, we discuss the stability. A new type of cycle is present, which we call a double S shaped cycle. In this cycle, the extent of accretion rate variability is much larger than that in dwarf nova cycles. This probably solves the problem of violent variability of AGN. In the meantime, the very high accretion rate at the hottest state in limit cycles in the unstable region may provide continuous injection of matter to the jet and power the relativistic motion of the jet.     

On the internal structure and magnetic fields of

First, a sequence of four-zone models for the interior of Venus is constructed under the assumption of hydrostatic equilibrium. While the equation of state for each zone is taken to be the Bullen's relation with its coefficients consistent with the PREM Earth model (Dziewonski and Anderson, 1981), the position of core-mantle boundary is determined by matching solutions of the Emden's equation in different regions. The results of hydrostatic models indicate the presence of a reasonably large molten iron core in Venus, broadly similar to the Earth. It is also found that the position of the core-mantle interface is nearly model-independent. Second, we focus on the question why Venus does not possess a significant global magnetic field and on what we can learn from this fact. Solutions of magnetohydrodynamic equations appropriate for the molten core of Venus are discussed. It is argued that, because the Elsasser number measuring the relative importance of Coriolis and Lorentz forces satisfies 1, equations for the problem of thermal convection in the Venusian fluid core must be nearly uncoupled with the dynamo equation. The existence of a global magnetic field, though small, then suggests that the size of the magnetic Reynolds numberR _m must beR _m =O(10), sustaining a dynamo action near its marginal state but not an active dynamo in the Venusian molten core. On the basis of asymptotic relations for finite amplitude convection, a useful constraint on important physical parameters for the liquid core of Venus is derived and discussed.     

Observations and interpretation of the close binary system CG Cyg

Photometric observations of the short-period (RS CVn-type) eclipsing binary system CG Cyg have been presented. Two sets of results, obtained from an analysis of theB, V andR light curves, represent ‘occultation’ and ‘transit’ solutions. The occultation solution is preferred as it gives a better fit to the colour curve. This hypothesis may also offer a more promising explanation of the observed peculiarities such as period changes and the light variation outside eclipses.     

Unusual coronal activity following the flare of 6 November 1980

For almost 30 hr after the major (gamma-ray) two-ribbon flare on 6 November 1980, 03:30 UT, the Hard X-Ray Imaging Spectrometer (HXIS) aboard the SMM satellite imaged in > 3.5 keV X-rays a gigantic arch extending above the active region over the limb. Like a similar configuration on 22 May 1980, this arch formed the lowest part of a stationary post-flare radio noise storm recorded at metric wavelengths at Nançay and Culgoora. 6.5 hr after the flare a coronal region below the arch started quasi-periodic pulsations in X-ray brightness, observed by several SMM instruments. These brightness variations had no response in the chromosphere (H), very little in the transition layer (O v), but they clearly correlated with similar variations in brightness at 169 MHz. There were 13 pulses of this kind, with apparent periodicity of about 20 min, until another flare occurred in the active region at 15:00 UT. All the brightenings appeared within a localized area of about 30000 km² in the northern part of the active region, but they definitely did not occur all at the same place.The top of the X-ray arch, at an altitude of 155 000 km, was continuously and smoothly decaying, taking no part in the striking variations below it. Therefore, the area variable in brightness does not seem to be the footpoint of the arch, as we supposed for similar variations on 22 May. More likely, it is a separate region connected directly with the source of the radio storm; particles accelerated in the storm may be dumped into the low corona and cause the X-ray enhancements. The X-ray arch was enhanced by two orders of magnitude in 3.5–5.5 keV X-ray counts and the temperature increased from 7.3 × 10⁶ to 9 × 10⁶ K when the new two-ribbon flare occurred at 15:00 UT. Thus, it is possible that energy is brought into the arch via the upper parts of the reconnecting flare loops - a process that can continue for hours.     

The character of the fine fractions of the bottom sediments of the Bohemian part of the Labe (Elbe) river

The amounts of fractions with size 63 m in the bottom sediments of the Bohemian part of the Labe (Elbe) river is variable. The grain-size distribution is poorly to very poorly sorted with a mean particle size from 5.1 to 12.2 m.The crystalline phase of recent sediments of the Labe river draining various types of parent rocks has a simple mineral composition, with predominant mica/illite, quartz, kaolinite and chlorite (±vermiculite ±smectite). There are also very small amounts of interstratification of 10 Å and 14 Å sheet silicates (11–13.6 Å). Small amounts of Na-rich plagioclase, K-feldspar and calcite have also been found; accessory amounts of amphibole, authigenic gypsum and pyrite are also present. Opal in diatomic tests is an omnipresent constituent. In the amorphic inorganic phase, hydrated oxides predominate, where Si >Al >Fe >Mn in a ratio of 64:28:22:1. The greatest amount of amorphous inorganic and organic phases were found in the 4 m fraction.A considerable part of the detrital material originates from soils formed on various igneous, metamorphic and sedimentary rocks, transported to the Labe by other rivers or directly by erosion in the immediate vicinity of the Labe river.