大头鳕和半滑舌鳎RAPD分析条件的优化

大头鳕和半滑舌鳎是我国重要的两种经济鱼类,本论文以大头鳕和半滑舌鳎基因组DNA为模板,对RAPD分析中的DNA模板浓度进行了优化,确定最适浓度。并分别筛选出20条和18条扩增结果稳定性好、多态性强的随机引物用于大头鳕以及半滑舌鳎的RAPD遗传变异分析。     

Scarifying and fingering surfaces of plunging jets

The local surface deformation resulting from the oblique impact of a columnar water jet has been computed, using a three-dimensional large eddy simulation, as a model of the overturning jet of a breaking wave. The emergence of the secondary jet from the front face of the initial jet has been examined and the organisation of the vortices within the jet characterised. As the secondary jet emerges, the vorticity field becomes unstable under the action of the strong shear beneath the jet surface and pairs of longitudinal counter-rotating vortices stretched along the direction of the jet projection are formed. The presence of these longitudinal vortex pairs creates convergent surface flows, resulting in the formation of longitudinal scars on the rear face of the projecting jet. Following significant growth of the scars on both its upper and lower surfaces, the jet decouples into fingers. The lateral widths of the longitudinal vortices provide a minimum measure of the finger size. A horizontal Froude number Fr_h, representing a measure of strength of horizontal shear in a gravity-dominated impacting flow is defined, which characterises the organisation of the longitudinal vortices occurring in the shear flow, and the resultant formation of scars and fingers. For higher Fr_h, stronger longitudinal vortices and deeper scars are formed at longer lateral intervals, enhancing the fingering process during the splashing event. Fundamental features of material transport in the vicinity of the surface of jets (e.g. gas transfer across a sea surface) are related to the entrainment of surface fluid by the longitudinal vortices, and is thus also characterised by Fr_h.     

Helium isotopic fingerprints of the heavy land subsidence left in groundwater of the Saga Plain,near Beppu–Shimabara graben,Kyushu, Japan

The Saga Plain is near Beppu–Shimabara graben, a region of potential active volcanism. In the graben, mantle He, which has a high ³He/⁴He ratio of 1.1 × 10⁻⁵, escapes easily from the underlying subduction zone. In groundwater of the Saga Plain, except in the Shiroishi district, this ratio gradually increased as the dissolved He content increased, to a maximum of 5 × 10⁻⁶. In central Shiroishi, however, the ratio reached a minimum of 8.7 × 10⁻⁷ with increasing dissolved He content, suggesting that groundwater in central Shiroishi has selectively accumulated radiogenic He, which has a very low ratio of 1 × 10⁻⁸, rather than reflecting the regional He, which is rich in mantle He. This can be explained if groundwater in Shiroishi has become mixed with fossil pore water drawn from impermeable marine clay aquitard layers. The withdrawal of pore water has also caused severe land subsidence in central Shiroishi.     

Effect of heavy oil on the development of the nervous system of floating and sinking teleost eggs

Heavy oil (HO) on the sea surface penetrates into fish eggs and prevents the normal morphogenesis. To identify the toxicological effects of HO in the context of the egg types, we performed exposure experiments using floating eggs and sinking eggs. In the course of development, HO-exposed embryos of floating eggs showed abnormal morphology, whereas early larva of the sinking eggs had almost normal morphology. However, the developing peripheral nervous system of sinking eggs showed abnormal projections. These findings suggest that HO exposed fishes have problems in the developing neurons, although they have no morphological malformations. Through these observations, we conclude that HO is strongly toxic to floating eggs in the morphogenesis, and also affect the neuron development in both floating and sinking eggs.     

Varying Fine-Structure Constant and the Cosmological Constant Problem

We start with a brief account of the latest analysis of the Oklo phenomenon providing the still most stringent constraint on time variability of the fine-structure constant α. Comparing this with the recent result from the measurement of distant QSO's appears to indicate a non-uniform time-dependence, which we argue to be related to another recent finding of the accelerating universe. This view is implemented in terms of the scalar-tensor theory, applied specifically to the small but nonzero cosmological constant. Our detailed calculation shows that these two phenomena can be understood in terms of a common origin, a particular behavior of the scalar field, dilaton. We also sketch how this theoretical approach makes it appropriate to revisit non-Newtonian gravity featuring small violation of Weak Equivalence Principle at medium distances. This revised version was published online in July 2006 with corrections to the Cover Date.     

Review on Morphological Insights of Self-Potential Anomalies on Volcanoes

Energy and thermal transfers in active volcanoes can play an important role incontrolling their dynamics depending on the hydrothermal state. Much geothermalenergy is released through the groundwater circulation, hot gas emission and thermalconduction. Therefore, it is very important to know the hydrological and thermalenvironments associated with volcanoes from the volcano-energetic point of view.However, it is difficult to evaluate these because of the availability of only a fewborehole data on the summit of volcanoes. Recent studies reveal that self-potential(SP) anomalies (up to some hundreds of mV) are observed on volcanoes, activefissure zones and/or fumarolic areas, suggesting that the SP anomalies are closelyrelated to heat-triggered phenomena such as thermoelectric and electrokinetic effectsdue to hydrothermal circulations. Therefore, SP studies can be appropriate for sensingthe thermal and hydrothermal states of volcanoes. In addition, monitoring SP anomaliescan be an efficient method for describing the change of thermal state and the evolutionof the hydrothermal (and volcanic) activities.In this paper, we have reviewed the origin of the SP anomalies associated withvolcanic phenomena theoretically as well as experimentally. Subsequently, wehave presented the results of many case studies and have classified the types ofanomalies in accordance with possible mechanisms. We have also described theresults of time variations of SP anomalies associated with volcanic activities. Timevarying SP fields exhibit the dynamic aspects of volcanic activities correspondingto the evolution of hydrothermal activity, changes in ground water circulation andmagma displacement. These morphological insights should lead to a quantitativeinterpretation of SP anomalies in volcanic regions.     

Crustal structure and tectonics of the Hidaka Collision Zone, Hokkaido (Japan), revealed by vibroseis seismic reflection and gravity surveys

This study is the first integrated geological and geophysical investigation of the Hidaka Collision Zone in southern Central Hokkaido, Japan, which shows complex collision tectonics with a westward vergence. The Hidaka Collision Zone consists of the Idon'nappu Belt (IB), the Poroshiri Ophiolite Belt (POB) and the Hidaka Metamorphic Belt (HMB) with the Hidaka Belt from west to east. The POB (metamorphosed ophiolites) is overthrust by the HMB (steeply eastward-dipping palaeo-arc crust) along the Hidaka Main Thrust (HMT), and in turn, thrusts over the Idon'nappu Belt (melanges) along the Hidaka Western Thrust (HWT). Seismic reflection and gravity surveys along a 20-km-long traverse across the southern Hidaka Mountains revealed hitherto unknown crustal structures of the collision zone such as listric thrusts, back thrusts, frontal thrust-and-fold structures, and duplex structures. The main findings are as follows. (1) The HMT, which dips steeply at the surface, is a listric fault dipping gently at a depth of 7 km beneath the eastern end of the HMB, and cutting across the lithological boundaries and schistosity of the Hidaka metamorphic rocks. (2) A second reflector is detected 1 km below the HMT reflector. The intervening part between these two reflectors is inferred to be the POB, which is only little exposed at the surface. This inference is supported by the high positive Bouguer anomalies along the Hidaka Mountains. (3) The shallow portion of the IB at the front of the collision zone has a number of NNE-dipping reflectors, indicative of imbricated fold-and-thrust structures. (4) Subhorizontal reflectors at a depth of 14 km are recognized intermittently at both sides of the seismic profile. These reflectors may correspond to the velocity boundary (5.9–6.6 km/s) previously obtained from seismic refraction profiling in the northern Hidaka Mountains. (5) These crustal structures as well as the back thrust found in the eastern end of the traverse represent characteristics of collisional tectonics resulting from the two collisional events since the Early Tertiary.     

Determination of silicate in seawater by inductively Coupled Plasma Atomic Emission Spectrometry

Inductively Coupled Plasma Atomic Emission Spectrometry (ICP) was adopted for direct analysis of silicate in seawater, eliminating necessity for pre-treatment. Via this method, the determination of silicate is rapid and easy compared with conventional methods of colorimetry. In seawater analysis, a decrease of sensitivity of about 24% was observed due to interference by coexisting elements, mainly Na. Examination of the analytical conditions revealed a detection limit of 0.3 M Si, and precisions of approximately 3.2, 2.0 and 1.3% for Si levels of 4, 15 and 94 M, respectively. Using this method, silicate determination in the East China Sea was attempted.     

Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus?

As basic research for the effect of heavy oil on the fish immune system, in this study, the number of leukocyte was counted in Japanese flounder Paralichthys olivaceus, after exposure to heavy oil at a concentration of 30 g/8 L for 3 days. To compare the numbers of bacteria in the skin mucus between oil-exposed and control fish, viable bacteria were enumerated by counting colony forming unit (CFU). Compared with 5.79 ± 1.88 × 10⁷ leukocytes/mL in the controls, the exposed fish demonstrated higher counts, averaging 1.45 ± 0.45 × 10⁸ cells/mL. The bacterial numbers of control fish were 4.27 ± 3.68 × 10⁴ CFU/g, whereas they were 4.58 ± 1.63 × 10⁵ CFU/g in the exposed fish. The results suggest that immune suppression of the fish occurred due to heavy oil stressor, and bacteria could invade in the mucus, resulting in the increasing leukocyte number to prevent infectious disease.