Molecular identification and culture observation on <Emphasis Type="Italic">Acrochaete leptochaete</Emphasis> (Chaetophoraceae,Chlorophyta) from China

Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species’ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied.     

Phytoplankton pigment patterns and community composition in the northern South China Sea during winter

Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the CHEMTAX software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.89±0.63 in the upper 50 m and 0.16±0.06 below 50 m depth. With increasing depth, APP decreased and photo-synthetically active radiation was attenuated. There was an obvious succession in the phytoplankton community from inshore to the open sea. Diatoms were dominant in the inshore region, while pelagophytes, Prochlorococcus, cyanobacteria and prymnesiophytes were dominant in the open sea. The vertical distribution of phytoplankton also differed greatly from inshore to the open sea. In the coastal and shelf region, diatoms were important components in the whole water column. Cyanobacteria also had a high abundance at the Subsurface Chlorophyll a Maxima (SCM) in the shelf region. In the slope and open sea, Prochlorococcus and cyanobacteria were important groups above the SCM, while pelagophytes dominated below the SCM.     

Nutrient and chlorophyll a anomaly in red-tide periods of 2003-2008 in Sishili Bay, China

Sishili Bay is the most important aquiculture and tourism area for the city of Yantai, China; however, red tides occurred frequently and have caused huge economic losses in this bay in recent years. To gain a better understanding of the local ecological environments in the bay, we conducted this research between 2003 and 2008 to analyze variations in nutrients and chlorophyll (chl-a) during high frequency red tide period (May to September). The results show that the chl-a concentration increased from 2.70 in 2003 to 7.26 mg/m3 in 2008, while the concentration of total inorganic nitrogen (TIN) and silicate (SiO3-Si) increased lineally from 5.18 and 1.45 μmol/L in 2003 to 18.57 and 9.52 μmol/L in 2008, respectively, and the annual phosphate (PO4-P) varied between 0.15 and 0.46 μmol/L. Special attention was given to a red tide in August 2007 occurred when water temperature was high and nutrient concentrations increased sharply because of a heavy rainfall. Overall, the results show the P limitation in Sishili Bay, and reveal that red tides were caused by eutrophication from terrestrial inputs and local warm weather, particularly during rainy periods. Therefore, to control red tide, greater efforts should be made to reduce sewage discharges into Sishili Bay, particularly during rainfall seasons.     

Rotary spectrum analysis of tidal current in the southern Yellow Sea

A complete set of one-month Acoustic Doppler Profiler (ADP) current data at a station in the southern Yellow Sea (SYS) is analyzed using the rotary spectrum method. The results revealed different rotary properties between barotropic and baroclinic tidal currents. The barotropic and baroclinic tidal currents rotate elliptically counter-clockwise and clockwise, respectively. Meanwhile, baroclinic bottom tidal currents are almost along-isobath. The baroclinic cross-isobath velocities attenuate quickly at the bottom, implying important effects of bottom topography on the cross-isobath motions.     

GROUNDWATER QUALITY AND CONTAMINATION INDEX MAPPING IN CHANGCHUN CITY, CHINA

1INTRODUCTIONGroundwater in Changchun City assures about 45% of total water supply. Drinking water supply of Chang-chun City was mostly served by surface water from Shitoukoumen and Xinlicheng reservoirs located in the east and south of the city (ZHANG, 1993). However, with the development of urban construction the ground-water especially from deep boreholes is also used for drinking purpose in suburban areas. The urbanization process in Changchun City threatens the groundwater quali…     

2000国家GPS大地控制网数据处理方法与结果

介绍了我国GPS大地控制网的基本情况,阐述了结合地壳运动观测网络工程建立全国统一的“2000国家GPS大地控制网”的意义,并介绍了整网平差中采用的技术方法和最后平差结果及精度。     

The Characteristic Patterns of Macrofaunal Fouling Assemblages in Nearshore Waters of the South China Sea

The spatio-temporal patterns of macrofaunal fouling assemblages were quantitatively investigated in the nearshore waters of the South China Sea. The work was undertaken by deploying seasonal panels at two sites (H-site, L-site) for one year, and the fouling communities on the panels were examined and analyzed. The results indicated that species composition of assemblages was obviously different between the two sites. At both sites the assemblages were characteristic with solitary dominant species throughout the year, with Amphibalanus reticulates dominating at H-site and Hydroides elegans at L-site. Shannon index and biomass of the assemblages varied with depth and season at both sites. At H-site the total biomass in summer and autumn were significantly higher than those in spring and winter, while at L-site the assemblage biomass also differed significantly among the four seasons, and the greatest biomass occurred at the depth of 2.0 m in winter. The abundance of all seasonal samples in non-metric multidimensional scaling was clustered as one group at L-site and three groups at H-site. The environmental factors were more likely to be related to the variation of fouling assemblages. Furthermore, it also suggests that in tropical seas the integrated adaptability would qualify a species for dominating a fouling assemblage despite its short life cycle, rather than the usually assumed only species with long life span. This study reveals the complexity and characteristic dynamics of macrofaunal fouling assemblages in the tropical habitats, and the results would provide valuable knowledge for biodiversity and antifouling research.     

����PCA������ǰ�����TEC�쳣

????IGS???????GIM???????????????TEC??Total Electron Content?????????2007-2011???????й??????????17???M??≥6.0????????????????????PCA??Principal Component Analysis?? ???????????TEC??????????????????????????б???????????PCA??????????????????????????????????????????￡???????????????TEC????????????????????????????0~5??     

Complete genome of Cobetia marina JCM 21022T and phylogenomic analysis of the family Halomonadaceae

Cobetia marina is a model proteobacteria in researches on marine biofouling. Its taxonomic nomenclature has been revised many times over the past few decades. ~To better understand the role of the surface-associated lifestyle of C. marina and the phylogeny of the family Halomonadaceae, we sequenced the entire genome of C. marina JCM 21022 ~T using single molecule real-time sequencing technology(SMR~T) and performed comparative genomics and phylogenomics analyses. ~The circular chromosome was 4 176 300 bp with an average GC content of 62.44% and contained 3 611 predicted coding sequences, 72 t RNA genes, and 21 r RNA genes. ~The C. marina JCM 21022 ~T genome contained a set of crucial genes involved in surface colonization processes. ~The comparative genome analysis indicated the significant diff erences between C. marina JCM 21022 ~T and Cobetia amphilecti KMM 296(formerly named C. marina KMM 296) resulted from sequence insertions or deletions and chromosomal recombination. Despite these diff erences, pan and core genome analysis showed similar gene functions between the two strains. ~The phylogenomic study of the family Halomonadaceae is reported here for the first time. We found that the relationships were well resolved among every genera tested, including Chromohalobacter, Halomonas, Cobetia, Kushneria, Zymobacter, and Halotalea.     

Characterizing landscape spatial heterogeneity in multisensor images with variogram models

Most evaluation of the consistency of multisensor images have focused on Normalized Difference Vegetation Index （NDVI） products for natural landscapes, often neglecting less vegetated urban landscapes. This gap has been filled through quantifying and evaluating spatial heterogeneity of urban and natural landscapes from QuickBird, Satellite pour l＇observation de la Terre （SPOT）, Ad- vanced Spacebome Thermal Emission and Reflection Radiometer （ASTER） and Landsat Thematic Mapper （TM） images with variogram analysis. Instead of a logarithmic relationship with pixel size observed in the corresponding aggregated images, the spatial variability decayed and the spatial structures decomposed more slowly and complexly with spatial resolution for real multisensor im- ages. As the spatial resolution increased, the proportion of spatial variability of the smaller spatial structure decreased quickly and only a larger spatial structure was observed at very coarse scales. Compared with visible band, greater spatial variability was observed in near infrared band for both densely and less densely vegetated landscapes. The influence of image size on spatial heterogeneity was highly dependent on whether the empirical sernivariogram reached its sill within the original image size. When the empirical semivariogram did not reach its sill at the original observation scale, spatial variability and mean characteristic length scale would increase with image size; otherwise they might decrease. This study could provide new insights into the knowledge of spatial heterogeneity in real multisen- sor images with consideration of their nominal spatial resolution, image size and spectral bands.