Nitrate assimilation by zooxanthellae maintained in laboratory culture

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.     

俄远东、中国东北的构造特点及岩石圈深部的不均一性

俄罗斯远东和中国东北前新生代可划分为８个大地构造单元。区域地质、深部地质、地球物理及新构造研究表明，地壳和地幔深部的不均一性反映了该区主要构造单元的深部构成和大地动力学等方面的特点。地热和地磁资料表明，远东地区软流层顶部具有起伏形态的特点，最大深度２００～２５０ｋｍ，浅部仅１００～１４０ｋｍ，松辽盆地７５～１００ｋｍ；在中国东北及亚太过渡带上地幔低速层厚度在０～１００ｋｍ之间变化，计算数据表明，本区地壳厚度为３０～４４ｋｍ，变化幅度为１４ｋｍ。     

Successful sidetracking of a well onshore Germany by integration of 3D vertical seismic profiling technology – a case study

The exploration for and exploitation of deep Lower Rotliegend gasfields onshore in North Germany often suffers from poor surface seismic imaging. This is owing to the depth of the reservoirs and a thick and complex Zechstein salt overburden. RWE Dea conducted a 3D vertical seismic profile (VSP) survey in a low‐performing production well after the borehole was plugged near total depth. Our main objective was to improve the seismic image of the reservoir zone in the vicinity of the well to determine a new landing point for a planned sidetrack. Because acquisition was in a densely populated and also partially environmentally protected area, there were surface restrictions concerning source deployment. Additionally, due to the complex geological setting, we conducted two 2D VSP field tests and thorough pre‐survey modelling to achieve the best results in terms of seismic imaging, environmental impact and reasonable cost. Deformation bands in the drill core suggest that the initial well was drilled close to a major fault, which was regarded as the main reason for the disappointing production rate. Therefore, we put special emphasis on fault detection in our processing and interpretation. Our interpretation approach used an enhanced structural mapping workflow that helped to design a sidetrack. When the sidetrack was drilled two years later, it ended up being one of the most productive wells in the field.     

Geochemistry and Nd,Sr isotopes of the Pohrenk fluorites (Kırsehir-Turkey)

The Pohrenk fluorite mineralisation which makes up Central Anatolia’s most extensive fluorite region is Lutetian aged, and hosted in carbonate rocks that have undergone occasional karstification and silicification along a N–S fault trend. Fluid inclusion values and the position of fluorites in the Tb/La–Tb/Ca diagram show that mineralisation occurred in a hydrothermal environment with homogenisation temperatures ranging from 78.1–363°C. The presence of fluorite as a space filler in carbonate rocks and its association with silicification indicates that the solutions contained considerable amounts of Si alongside F (fluorine). The Pohrenk fluorite samples have ¹⁴³Nd/¹⁴⁴Nd values of between 0.512349 and 0.512497, whilst ⁸⁷Sr/⁸⁶Sr values vary between 0.708161 and 0.708772. These values indicate a mantle origin where continental contamination could occur. When the Nd–Sr values are compared to magmatic and young volcanics, the Pohrenk fluorites are seen to be enriched and exhibit similar isotopic signatures to Upper Cretaceous aged magmatics, Early-Middle Miocene volcanics and Mio-Quaternary volcanics.     

Geotechnical aspects of fine-grained mass flow deposits: California Continental Borderland

Seismic reflection profiling demonstrates the importance of mass movement in the north-central California Borderland. Bulk properties of surficial hemipelagic sediments in the Santa Barbara, Santa Cruz, and San Nicolas basins show that different types of mass-transported sediments have different properties, although their general sedimentologic characteristics are very similar. Slump deposits have moderate water contents (48% wet weight basis), high plasticity indices (46%), and moderate activities (1.6). For debris flows these values are low: 28%, 5%, and 0.3, respectively, and for other types (liquefied flow, mudflows, and turbidities) the values are high: 54 to 63%, 49 to 58%, and 1.2 to 1.5, respectively.     

Advances in the fatigue assessment of wire ropes

This paper presents the findings from an in-depth analysis of the (axial) stiffness data recorded during tension–tension fatigue tests on wire ropes, particularly in relation to how changes in stiffness during testing relate to changes in rope strength. A linear relationship between stiffness and strength is shown to exist and a methodology presented for quantifying residual strength with applied cycles. New lower bound fatigue lines for six-strand rope and spiral strand are presented which are based on a 10% loss of strength. These new lines have the advantage of having been established using a common discard criterion for wire ropes.     

Trace elements in organic- and iron-rich surficial fluids of the boreal zone: Assessing colloidal forms via dialysis and ultrafiltration

On-site size fractionation of about 40 major and trace elements (TE) was performed on waters from boreal small rivers and their estuaries in the Karelia region of North-West Russia around the “Vetreny Belt” mountain range and in Paanajärvi National Park (Northern Karelia). Samples were filtered in the field using a progressively decreasing pore size (5 μm, 2.5 (3) μm, 0.22 (0.45) μm, 100 kDa, 10 and 1 kDa) by means of frontal filtration and ultrafiltration (UF) techniques and employing in-situ dialysis with 10 and 1 kDa membranes followed by ICP-MS analysis. For most samples, dialysis yields a systematically higher (factor of 2-3) proportion of colloidal forms compared to UF. Nevertheless, dialysis is able to provide a fast and artefact-free in-situ separation of colloidal and dissolved components.Similar to previous studies in European subarctic zones, poor correlation of iron concentration with that of organic carbon (OC) in (ultra)filtrates and dialysates reflect the presence of two pools of colloids composed of organic-rich and Fe-rich particles. All major anions and silica are present as dissolved species (or solutes) passing through the 1-kDa membrane. Size-separation ultrafiltration experiments show the existence of larger or smaller pools of colloidal particles different for each of the considered elements.The effect of rock lithology (acidic versus basic) on the colloidal speciation of TE is seen solely in the increase of Fe and some accompanying TE concentrations in catchment areas dominated by basic rocks compared to granitic catchments. Neither the ultrafiltration pattern nor the relative proportions of colloidal versus truly dissolved TE are affected by the lithology of the underlying rocks: within ±10% uncertainty, the two colloidal (10 kDa-0.22 μm and 1-10 kDa) and the truly dissolved (<1 kDa) pools show no difference in percentage of TE distribution between two types of bedrock lithology. The same conclusion is held for organic- and Fe-rich waters. In contrast, landscape context analysis demonstrated slight dominance, for most TE affected by UF, of large-size colloids (10 kDa-0.22 μm) in rivers and streams and small-size colloids and truly dissolved fractions in swamp stagnant surface waters. This supports the existence of two pathways of colloids formation: during the plant litter degradation in wetland zones and at the redox front in river riparian zone.