合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Modeling of Multi-Freedom Ship Motions in Irregular Waves with Fuzzy Neural Networks

1 .IntroductionAshiptravelingatseaundergoesundesirablewave inducedmotions ,namely ,surge ,sway ,heav ing ,rolling ,pitchingandyaw .Thesemotionsoftencauseproblemstothecrew ,theonboardequip mentand ,intheworstcase ,thesafetyofthevessel.Tominimizethewave inducedshipmotions ,controlsystemsmaybeapplied .Theaccuratemodelingofshipmotionsisthereforeveryimportantforshipdesignanddesignofmotioncontrolsystems .Manyresearchershavedevelopedshipmotionpredictionmethodsbasedonthepotentialflowtheo ries (Dong ,…     

A New Probability of Detection Model for Updating Crack Distribution of Offshore Structures

1 .IntroductionNondestructiveinspection (NDI)isveryimportantforensuringthereliabilityofoffshorestructuresintheirservicelives (Lauraetal.,1 996 ) .Itiswellknownthatdetectionofflawsinvolvesconsider ablestatisticaluncertainties.Asaresult,theprobabilityofdetection (POD)forallflawsofagivensizehasbeenusedintheliteraturetodefinethecapabilityofaparticularNDItechniqueinagivenen vironment.SincethedataofPODusuallyscatterlargely ,itisdifficulttodeterminewhichmodelfitstheavailabledatabest.Thismodelun…     

北黄海沉积物中重金属分布及环境背景值

本文讨论了北黄海１１个柱状岩芯样的颗粒物（＜０．０６３ｍｍ）中重金属的分布，采用统计学方法对Ｃｕ，Ｐｈ，Ｚｎ和Ｃｄ进行态性检验，用柱样稳定段（拐点以下）作为背景段，获得重金属环境背景值．     

山东省大中型水库浮游生物研究──Ⅰ．浮游动物生态

报道１９８９～１９９０年山东省３０多个大中型代表水库浮游动物的种类组成，种类频级、优势类群。主要种和生物量；统计分析掠食性与植食性浮游动物生物量，浮游动物与浮游植物生物量及浮游动物与代表水库营养状态指标的总磷，透明度和深度的关系。提出浮游动物个体越小，其生物量与水库营养水平关系越密切。     

Full-range nonlinear analysis of fatigue behaviors of reinforced concrete structures by finite element method

Ｆｕｌｌ－ｒａｎｇｅｎｏｎｌｉｎｅａｒａｎａｌｙｓｉｓｏｆｆａｔｉｇｕｅｂｅｈａｖｉｏｒｓｏｆｒｅｉｎｆｏｒｃｅｄｃｏｎｃｒｅｔｅｓｔｒｕｃｔｕｒｅｓｂｙｆｉｎｉｔｅｅｌｅｍｅｎｔｍｅｔｈｏｄ￥ＳｏｎｇＹｕｐｕ；ＺｈａｏＳｈｕｎｂｏ；ＷａｎｇＲｕｉｍ...     

辽东湾海底残留地貌和残留沉积

全新世以来,世界海面大幅度上升,渤海海区发生了明显的海侵,玉木冰期低海面时期的近海广大陆域皆沉溺于海平面以下。辽东湾由于地质构造复杂及沉积作用速度分布不均匀等因素,海底保存了一部分玉木冰期低海面时期陆地或滨海区的地貌形态及相应的老沉积物的遗迹。它们虽然经过了后期水动力条件及沉积作用的改造,但是,仍不同程度地反映出原始地貌的迹象和某种残留沉积的特征。 关于辽东湾残留地貌和残留沉积的问题,过去曾有报道;本文在以往调査的基础上,结合近期有关资料,对此作进一步的探讨。     

鱼类生长激素的体内代谢、分泌调控和作用机制研究的进展

主要对１９８３－１９９６年间关于鱼类生长激素的体内代谢、分泌调控和作用机制的研究进展予以综合评述。通过鱼类划性放射免疫测定法的建立和体风代谢动力学研究，证实，外源生长激素在鱼体内不会产生积累。鱼类生长激素的分泌调控受下丘脑分泌的生长激素释放因子（ＧＨＲＦ）和释放抑制因子（ＳＲＩＦ）的双重；促性腺激素释放激素（ＧｎＲＨ）、雌二醇（Ｅ２）、促甲状腺素释放激素（ＴＲＨ）和神经肽（ＮＹＰ）对鱼类生长激素的     

Action of Cnoidal Waves on Vertical Walls

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves     

艾比湖地区冰消期以来气候演化的植物学证据

本文根据植物生态学分析方法，通过对艾比湖ＺＫｏｏＢ孔中孢粉组合及植被生态的定量分析，阐述了北疆内陆干旱区冰消期以来的气候演化状况。孢粉组合特征及其植被生态的定量分析研究表明，受大气降水以及大气和土壤中相对湿度波动变化的影响，艾比湖地区的生态环境特征在最近１５０００ａ中至少经历了１０次比较明显的波动变化，并且与全球气候变化过程基本符合，表明中国西部干旱半干旱地区晚更新世以的气候环境演化过程是在全球变