N and O isotope effects during nitrate assimilation by unicellular prokaryotic and eukaryotic plankton cultures

In order to provide biological systematics from which to interpret nitrogen (N) and oxygen (O) isotope ratios of nitrate (¹⁵N/¹⁴N, ¹⁸O/¹⁶O, respectively) in the environment, we previously investigated the isotopic fractionation of nitrate during its assimilation by mono-cultures of eukaryotic algae (Granger et al., 2004). In this study, we extended our analysis to investigate nitrate assimilation by strains of prokaryotic plankton. We measured the N and O isotope effects, ¹⁵ε and ¹⁸ε, during nitrate consumption by cultures of prokaryotic strains and by additional eukaryotic phytoplankton strains (where ε is the ratio of reaction rate constants of the light vs. heavy isotopologues, ^lightk and ^heavyk; ε = ^lightk/^heavyk − 1 × 1000, expressed in per mil). The observed ¹⁵ε ranged from 5‰ to 8‰ among eukaryotes, whereas it did not exceed 5‰ for three cyanobacterial strains, and was as low as 0.4‰ for a heterotrophic α-protoeobacterium. Eukaryotic phytoplankton fractionated the N and O isotopes of nitrate to the same extent (i.e., ¹⁸ε ∼ ¹⁵ε). The ¹⁸ε:¹⁵ε among the cyanobacteria was also ∼1, whereas the heterotrophic α-proteobacterial strain, which showed the lowest ¹⁵ε, between 0.4‰ and 1‰, had a distinct ¹⁸ε:¹⁵ε of ∼2, unlike any plankton strain observed previously. Equivalent N vs. O isotope discrimination is thought to occur during internal nitrate reduction by nitrate reductase, such that the cellular efflux of the fractionated nitrate into the medium drives the typically observed ¹⁸ε:¹⁵ε of ∼1. We hypothesize that the higher in the ¹⁸ε:¹⁵ε of the α-proteobacterium may result from isotope discrimination by nitrate transport, which is evident only at low amplitude of ε. These observations warrant investigating whether heterotrophic bacterial assimilation of nitrate decreases the community isotope effects at the surface ocean.     

ON THE GENERATION OF P AND S WAVE ENERGY IN CRYSTALLINE ROCKS*

A seismic source consisting of a 700 kg weight that could be dropped vertically or projected down a ramp inclined at 45° to the vertical was tested as a source of P, SV and SH waves within a crystalline rock body at Chalk River, Ontario. The seismic energy was recorded by arrays of both horizontal and vertical-component geophones at distances between 30 and 600 m from the source, which was operated over glacial overburden varying in thickness from less than a meter to a few tens of meters. Seismic energy was more efficiently generated when the overburden thickness was at least several meters. The signals identified visually as S are generally true S, though some may be the converted wave PS. The SV amplitudes are generally larger than those of P, regardless of the type of shot, while the signal frequencies are roughly 60 Hz and 90 Hz, respectively. The horizontal-component seismograms for the inclined shots showed no evidence of SH polarization, and the SH amplitudes were only rarely enhanced relative to P and SV amplitudes on changing from vertical to inclined shots. These unexpected results are attributed to the combined effect of the high velocity and density contrasts and the irregularity of the boundary between the glacial overburden and the rock body.     

Facies Structure and Quantitative Parameters of Pleistocene Sediments of the Bering Sea

Lithofacies zoning is described for the first time for the Neo- and Eopleistocene of the Bering Sea. Four lithofacies sedimentation zones are distinguished: (I) terrigenous; (II) siliceous–terrigenous; (III) siliceous, and (IV) volcanoterrigenous ones. Corresponding maps were treated using Ronov volumetric method to quantify sedimentation parameters for distinguished lithofacies zones (subzones) and types of Pleistocene sediments. It was revealed that terrigenous sediments predominate over other sediments. Accumulation of the terrigenous sediments was more intense (by 1.4 times) in the Neopleistocene than in the Eopleistocene. The sedimentation rate of siliceous sediments of the Bowers Ridge in the Eopleistocene was two times higher than in the Neopleistocene.     

Tsunamis as geological agents

When a tsunami wave series approaches and interacts with a coast, the consequent passage shorewards of great volumes of water and their invasion of the land, especially within bays and up river valleys, results in the disturbance of existing sediment and the removal seawards of land debris and coastal and shallow‐water marine sediments. Tsunami action builds up sequences of peculiar sediments in shallow water; it at least assists in the formation and maintenance of submarine canyons and, through them, produces turbidity currents of a particularly powerful kind. Tsunami action may explain many puzzling sedimentary phenomena, for example, sudden and drastic changes in near‐shore bathymetry; the formation of chaotic sediments such as some paraconglomerates and edgewise conglomerates. It offers solutions to problems arising from the study of turbiditic sequences, both modern and ancient.     

The Upper Middle Rhine Valley as a risk area

The Upper Middle Rhine Valley, granted the status of a World Heritage site, is well known for its unique inner narrow valley of Quaternary age with its historical legacy of numerous medieval castles and old towns. Less known is that this has always been a risk area of floods and gravitative mass movements. Up to the recent past, mainly ice floods caused enormous damage. The inhabitants of the valley were well aware that they lived in a risk area, but they had learned to handle the flood hazard. With the demise of ice floods over the last 40 years, due to climate change and because of the additional heating of the river water by power plants, the awareness of flood hazards has been much diminished, in contrast to that of potential damage by rockfalls and landslides which were also much feared in the past, though at the local level only. Still in the people’s memory is the Kaub catastrophe of March 10, 1876, when 28 persons were killed by a landslide. Nowadays, even minor rockfalls are a major threat, as they will affect the much-used traffic lines on both banks of the river, in particular the railroads. Therefore, since 2002, on behalf of German Rail (Deutsche Bahn, DB), all problematic slopes have been protected by costly steel-ring nets, although they are an aesthetic problem by UNESCO standards. The feeling of absolute safety created among the public is only subjective, though, as planners are well aware of. Moreover, the impact of modern climate change on slope stability is nearly unknown. Therefore, it is still necessary to develop a risk map for the narrow valley, with emphasis on gravitational hazards.     

A mass ratio limit for primordial black holes

We augment our scenario for the formation of astronomical objects from macroscopic superstrings by the assumption that the central matter keeps its identity in the fragmentation. From the condition that the angular momentum per mass squared of this matter should be less than the Kerr limit G/c, we obtain upper limits for the ratio of the mass of central black holes M(BH) to the mass M of the host object. This limit is M(BH)/M ≈ 0.001, and, expressed in observed quantities, approximately M(BH)/M ≈ σ²/(v · c) where σ is the r.m.s. velocity, v the rotational velocity and c the velocity of light. The valuesM(BH) agree with the observed behaviour both in order of magnitude and in the variation with velocity dispersion. (© 2003 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

The role of prior model calibration on predictions with ensemble Kalman filter

This paper, based on a real world case study (Limmat aquifer, Switzerland), compares inverse groundwater flow models calibrated with specified numbers of monitoring head locations. These models are updated in real time with the ensemble Kalman filter (EnKF) and the prediction improvement is assessed in relation to the amount of monitoring locations used for calibration and updating. The prediction errors of the models calibrated in transient state are smaller if the amount of monitoring locations used for the calibration is larger. For highly dynamic groundwater flow systems a transient calibration is recommended as a model calibrated in steady state can lead to worse results than a noncalibrated model with a well-chosen uniform conductivity. The model predictions can be improved further with the assimilation of new measurement data from on-line sensors with the EnKF. Within all the studied models the reduction of 1-day hydraulic head prediction error (in terms of mean absolute error [MAE]) with EnKF lies between 31% (assimilation of head data from 5 locations) and 72% (assimilation of head data from 85 locations). The largest prediction improvements are expected for models that were calibrated with only a limited amount of historical information. It is worthwhile to update the model even with few monitoring locations as it seems that the error reduction with EnKF decreases exponentially with the amount of monitoring locations used. These results prove the feasibility of data assimilation with EnKF also for a real world case and show that improved predictions of groundwater levels can be obtained.     

X-ray transient sources

The galactic distribution and physical nature of X-ray transient sources is investigated. Two types of transients are considered. The observational data on 41 X-ray transient sources are given, and the average parameters of hard and soft X-ray transients are estimated.     

Observation of Precipitating Systems over Complex Orography with Meteorological Doppler Radars: A Feasibility Study

Summary  This paper concerns the use of airborne or ground-based Doppler radars to observe precipitating systems over complex orography. As nearly all of the previous experiments involving Doppler radars were conducted over flat surfaces over the continents or the oceans, new techniques are needed firstly to separate ground clutter from meteorological signal and, in the case of airborne Doppler observations, to deduce navigational errors. Secondly, it is necessary to take the atmospheric circulation induced by orography into account in the three-dimensional wind field analysis. Variational techniques are presented to solve these problems. The proposed methods are tested with simulated ground-based and airborne Doppler radar observations for analytic flows over analytic terrains and for numerically simulated wind and reflectivity fields for the Brig event (22 September 1993) of heavy precipitation over the southern flank of the Alps (Cosma and Richard, 1998), and with actual airborne Doppler data relative to weak snow showers over the Rocky Mountains on 12 March 1995. Received March 22, 1999/Revised June 1, 1999