Identification of phase and sex-related ISSR markers of red algaGracilaria lemaneiformis

Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.     

Design ofVibrio 16S rRNA gene specific primers and their application in the analysis of seawaterVibrio community

The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.     

FEM for stability analysis against overturning of portal water injection sheet pile

Portal water injection sheet pile （PWISP）, as a retaining wall, appeared in seashore engineering in 2000. Although there have been many systematic methods addressing the issue, there are very few focusing on the new structure because of the difficulties in defining the earth pressure between the two piles. A new method is proposed in this paper to obtain the earth pressure between the PWISPs. Stability analysis against overturning follows as a consequence. Using Finite Element Analysis （FEA） software ANSYS, both the nonlinear characteristics of the soil and those of the contact elements are taken into account to obtain the earth pressure distribution on the contact surface. Based on the results of the FEA, Rankin＇s theory and the slip plane theory, the formula of the earth pressure on the inner surfaces between the piles is given. Assuming the PWISP as the analysis object and the earth pressure as an outside force acting upon it, the equation of stability against overturning of the PWISP is presented. Finally, some parameters are discussed about the stability of the PWISP against overturning, such as the embedded depth of the front pile, the distance between the two rows of piles, the internal friction angle and the cohesion of the earth. The results show that the increase of the cohesion and the internal friction angle will decrease the distance and the embedded depth, and therefore enhance the stability against overturning. Specifically, when the distance is 1/3-2/3 of the maximal excavation depth, the two rows of piles give the best performance in stability.     

基于华北区域地震活动性分布的地震危险性评价模型

采用了分布式地震活动性模型. 该模型无需潜在震源区划分,同时简化了地震危险性概率分析方法. 根据破坏性地震目录建立了3个地震活动性模型,利用高斯光滑函数获得了华北区域内的a值分布特征,使用3种典型的衰减模型,分别计算了50年内超越概率10%, 5%和2%的地震动峰值加速度分布. 其分析结果显示了峰值加速度分布特征与我国第四代区划图大体一致,特定地震活动区（太原、 石家庄等地区）的峰值加速度略高于第四代区划图的结果,而这种峰值加速度分布特征与该地区较高的地震活动性特征是一致的. 概率危险性曲线结果表明,唐山、太原和北京等地区的潜在地震危险性比华北区域内其它城市高.      

面向对象的高分辨率影像城市建筑物提取

依据高分辨率遥感影像的特点,采用面向对象方法提出"自下而上"的多尺度分割方法,即按照由小尺度分割至大尺度分割的顺序,并结合掩膜操作对山东师范大学IKONOS影像进行了建筑物提取试验。结果表明,该方法较传统的"自上而下"的尺度分割方法精度有显著改善,其分类结果形状较为规整,更接近实际地物。     

对古地理再造中古地磁方法的回顾与探讨

古地磁数据的获取和应用是进行古地理再造的重要基础.同时,计算机技术的发展以及GIS技术的推广,极大地推动了古地理再造方法的革新,为古地理再造提供了更加方便、高效的软件辅助平台.本文主要围绕古地磁数据资料、应用方法及软件开发等方面,对其发展历史与研究现状作一详细的回顾与探讨.     

A star-based method for precise wavelength calibration of the Chinese Space Station Telescope(CSST) slitless spectroscopic survey

The Chinese Space Station Telescope(CSST)spectroscopic survey aims to deliver high-quality low-resolution(R>200)slitless spectra for hundreds of millions of targets down to a limiting magnitude of about 21 mag,distributed within a large survey area(17500 deg2)and covering a wide wavelength range(255-1000 nm by three bands GU,GV,and GI).As slitless spectroscopy precludes the usage of wavelength calibration lamps,wavelength calibration is one of the most challenging issues in the reduction of slitless spectra,yet it plays a key role in measuring precise radial velocities of stars and redshifts of galaxies.In this work,we propose a star-based method that can monitor and correct for possible errors in the CSST wavelength calibration using normal scientific observations,taking advantage of the facts that(ⅰ)there are about ten million stars with reliable radial velocities now available thanks to spectroscopic surveys like LAMOST,(ⅱ)the large field of view of CSST enables efficient observations of such stars in a short period of time,and(ⅲ)radial velocities of such stars can be reliably measured using only a narrow segment of CSST spectra.We demonstrate that it is possible to achieve a wavelength calibration precision of a few km s^-1 for the GU band,and about 10 to 20 kms^-1 for the GV and GI bands,with only a few hundred velocity standard stars.Implementations of the method to other surveys are also discussed.     

应用混合分布研究银川平原地下水埋深对植被的影响

银川平原地处西北干旱地区,自然生态环境在西北地区具有典型性。研究银川平原的生态环境,需要研究植被,它反映多种因素的作用。在影响植被的各种因素中,地下水对植被生长的影响非常重要,但将地下水对植被生长的影响进行定量化分离并不容易。近年来,用混合分布函数处理大量数据的方法受到越来越多的关注,但是混合分布函数的参数求解一直比较困难。文章提出了一种基于混合分布函数和数据直方图之间的关系,用最小二乘法估计参数的新方法;并用这种方法,结合银川平原遥感数据中的归一化植被指数NDVI,对地下水埋深与植被生长的关系进行定量分析,得到地下水埋深对植被影响所服从的正态分布密度函数。     

强震短临前兆异常共性特征的物理基础

强震短临阶段前兆异常的共性特征是"一多、二大、三广、四平静".通过讨论"一多、二大、三广"这三项微观前兆异常特征的物理基础,提出用"软包体模型"和信息论中系统处于临界点附近时信息会突然增强的理论,可以对上述共性特征做出解释.     

新疆黑英山-查汗沙拉-地那达坂地区锑矿成矿远景评价

我国西天山南段的黑英山-查汗沙拉-地那达坂锑多金属矿化区为中亚南天山成矿带的东延部分,区内广泛分布有泥盆系滨浅海含碳碎屑岩和碳酸盐岩,岩石具有较高的Sb背景场,异常多呈Sb、Au、As、Hg等元素组合异常出现,异常区内已发现有查汗沙拉、阿特达坂和地那达坂等多个锑矿(点)床.区内锑矿化及异常均受近Nw向大型逆冲推覆构造系统控制,具有良好的成矿条件和较大的找矿潜力.