Increased potential for NAD(P)H-dependent reactive oxygen species production of hepatic subcellular fractions of fish species with in vivo exposure to contaminants.

The present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ROS) production in disease processes in fish. NAD(P)H-dependent ROS production of subcellular fractions was determined by the iron/EDTA-mediated oxidation of 2-keto-4-methiolbutyric acid. Hepatic cytosolic NADPH-dependent and microsomal NAD(P)H-dependent ROS production were increased 51-160% (P < 0.05) in rainbow trout (Oncorhynchus mykiss) 15 weeks after a single i.p. injection of polychlorobiphenyl (PCB) (100 mg Clophen A50 kg-1 wet wt.). Hepatic microsomal NADH-dependent ROS production was 114% higher in perch (Perca fluviatilis) from PCB-contaminated Lake J?rnsj?n compared to clean Lake V?nern, Sweden. Hepatic mitochondrial NADH-dependent, cytosolic NADH-dependent and microsomal NADPH-dependent ROS production were variously elevated up to 160% in flounder (Platichthys flesus) at various sites along two pollution transects near to the ports of Rotterdam and Amsterdam, Netherlands. Overall the data indicate increased potential for ROS production in liver of fish exposed to field pollution, and support the hypothesis of oxidative stress as a mechanism of contaminant-mediated disease in fish.     

Suspended particulate matter and nepheloid layers over the southern margin of the Cretan Sea (N.E. Mediterranean): seasonal distribution and dynamics

Seawater along the southern margin of the Cretan Sea (May 1994–September 1995) has been found to have light transmission values ranging from 79% to 94%, corresponding to SPM values ranging from 1.5 mg l⁻¹ to 0.2 mg l⁻¹. The highest SPM concentrations (mostly of terrigenous origin) were found close to the sea-bed over the shelf-break and upper slope. The origins of SPM in the surface waters (<150 m) is principally biogenic. The occurrence of nepheloid layers at intermediate depths within the upper water column is mostly a result of density stratification. The dynamics of SPM distributions are governed by the 2-gyre system which induces a general onslope flow; and so inhibits the seaward dispersion of the relatively more turbid coastal/shelf waters. This is in agreement with the virtual absence of suspensates of terrigenous origin offshore of the shelf-break. Near bottom nepheloid layers (BNL) and detached intermediate nepheloid layers occur in the vicinity of the shelf-break and over upper slope region; these may be explained by resuspension induced by near-bed current activity and breaking of internal waves. High concentrations of SPM near the seabed may be caused by anthropogenic (trawling) activity. Occasionally, the formation of BNL may result from local seismic activity resulting in gravity-driven mass movements.     

Phytoplankton, nutrients and hydrography in the frontal zone between the Southwest Indian Subtropical gyre and the Southern Ocean

A survey was made of the Southwest Indian Ocean frontal region between 30 and 50°E containing the Agulhas Return, Subtropical and Subantarctic Fronts. From CTD, SeaSoar and extracted samples the distribution of nitrate, silicate and chlorophyll a is shown to be strongly linked to the front and water mass structure, varying zonally and meridionally. Surface chlorophyll a concentrations were low to the north and south leaving a band of elevated chlorophyll between the Subtropical and Subantarctic Fronts. The low concentration of chlorophyll a to the north, in Subtropical Water, was clearly due to nitrate limitation. Between the Subtropical and Subantarctic Fronts, where the chlorophyll a concentrations were highest, the surface layer showed silicate depletion limiting diatom growth. South of the Subantarctic Front there were deep extending, low concentrations of chlorophyll a, but despite plentiful supplies of macro-nutrients and a well-stratified surface layer, high concentrations of chlorophyll a were absent. Changes from west to east were associated with the meandering of the Southern Ocean Fronts, especially the Subtropical Front, and their strength and proximity to each other. Concentrations of chlorophyll a peaked where the Agulhas Return, Subtropical and Subantarctic Fronts were in close proximity. Combined frontal structures appear to have particularly pronounced vertical stability and are associated with enhanced upwelling of nutrients and leakage of nutrients across the front. Light levels are high within the shallow stable layer. Such conditions are clearly favourable for biological growth and support the development of larger-celled phytoplankton communities.     

Holocene sedimentation of a wave-dominated barrierisland shoreline: Cape Lookout, North Carolina

The sedimentary record of 130 km of microtidal (0.9 m tidal range) high wave energy (1.5 m average wave height) barrier island shoreline of the Cape Lookout cuspate foreland has been evaluated through examination of 3136 m of subsurface samples from closely spaced drill holes. Holocene sedimentation and coastal evolution has been a function of five major depositional processes: (1) eustatic sea-level rise and barrier-shoreline transgression; (2) lateral tidal inlet migration and reworking of barrier island deposits; (3) shoreface sedimentation and local barrier progradation; (4) storm washover deposition with infilling of shallow lagoons; and (5) flood-tidal delta sedimentation in back-barrier environments.

Twenty-five radiocarbon dates of subsurface peat and shell material from the Cape Lookout area are the basis for a late Holocene sea-level curve. From 9000 to 4000 B.P. eustatic sea level rose rapidly, resulting in landward migration of both barrier limbs of the cuspate foreland. A decline in the rate of sea-level rise since 4000 B.P. resulted in relative shoreline stabilization and deposition of contrasting coastal sedimentary sequences. The higher energy, storm-dominated northeast barrier limb (Core and Portsmouth Banks) has migrated landward producing a transgressive sequence of coarse-grained, horizontally bedded washover sands overlying burrowed to laminated back-barrier and lagoonal silty sands. Locally, ephemeral tidal inlets have reworked the transgressive barrier sequence depositing fining-upward spit platform and channel-fill sequences of cross-bedded, pebble gravel to fine sand and shell. Shoreface sedimentation along a portion of the lower energy, northwest barrier limb (Bogue Banks) has resulted in shoreline progradation and deposition of a coarsening-up sequence of burrowed to cross-bedded and laminated, fine-grained shoreface and foreshore sands. In contrast, the adjacent barrier island (Shackleford Banks) consists almost totally of inlet-fill sediments deposited by lateral tidal inlet migration. Holocene sediments in the shallow lagoons behind the barriers are 5–8 m thick fining-up sequences of interbedded burrowed, rooted and laminated flood-tidal delta, salt marsh, and washover sands, silts and clays.

While barrier island sequences are generally 10 m in thickness, inlet-fill sequences may be as much as 25 m thick and comprise an average of 35% of the Holocene sedimentary deposits. Tidal inlet-fill, back-barrier (including flood-tidal delta) and shoreface deposits are the most highly preservable facies in the wave-dominated barrier-shoreline setting. In the Cape Lookout cuspate foreland, these three facies account for over 80% of the sedimentary deposits preserved beneath the barriers. Foreshore, spit platform and overwash facies account for the remaining 20%.     

A detailed survey near Mount Eratosthenes Eastern Mediterranean

A detailed marine survey made to the South West of Mount Eratosthenes is described. The results show the existence of a narrow zone of WNW trending deformed sediment west of the mount surrounded by an evaporite sequence in different stages of deformation.This work was intended to settle the conflict between the results of three previous interpretations of the tectonic structure of this area. We see the area being characterised by five areas with different degrees of tectonic deformation yet showing little causal relationship to overburden of Nile Cone sediments. A fault controlled cliff coincides with the previously described salt wall and strike slip fault yet we see no evidence for its diapiric origin nor positive evidence for strike slip motion along it. We concur with the previously observed area of higher free-air gravity anomalies termed the Eratosthenes tectonic zone, although it is traversed by several boundaries between areas of different deformation. Our results thus show some agreements and some re-interpretations of the observations. This complicated region is not yet fully understood however.     

Nankai Trough: A hot trench?

Heat flow estimated from the gas hydrate layers on the landward slope of the Nankai Trough reveals that heat flow increases downslope toward the trench floor. This data plus six new heat flow values obtained by a conventional probe and two values available from DSDP drill holes give a fairly detailed heat flow distribution in the Nankai Trough area, when combined with the already existing data set. There appears to be a zonal pattern parallel to the trough axis, with a high heat flow zone on the floor of the trough that is quite anomalous for a subduction zone. It might be explained as a result of subduction of the hot portion of the Philippine Sea plate, i.e. the Shikoku Basin, and/or of more local effects such as heating due to intrusion of hot water from subducted sediments to shallow depth beneath the trough floor. Surface heat flow patterns landward of the trough were calculated for a simple thermal model of subduction. Perfect reproduction of the observed zonal pattern is difficult to achieve by the simple model, suggesting the necessity for further heat flow and other observations.     

Tectonics at the intersection of the East Pacific Rise with Tamayo Transform fault

During the Fall of 1979, a manned submersible program, utilizing DSRV ALVIN, was carried out at the intersection of the East Pacific Rise (EPR) with the Tamayo Transform boundary. A total of seven dives were completed in the vicinity of the EPR/Tamayo intersection depression and documented the geologic relationships that characterize the juxtaposition of these types of plate boundaries. The young volcanic terrain of the EPR axis can be traced into and across the Tamayo Transform valley but becomes buried by sedimentary talus that is being shed from sediment scarps along the unstable sediment slope that defines the north side of the intersection depression. Within 4 km of the transform boundary, the dominant trend (000°) of the fissures and faults that disrupt the rise-generated volcanics is markedly oblique to the regional direction of sea floor spreading (120°). Since no evidence was found to suggest that these structures accommodate significant amounts of strike-slip displacement, they are taken to reflect a distortion of the EPR extensional tectonic regime by a transform generated shear couple. The floor of the Tamayo Transform valley in this area is inundated by mass-wasted sediment, and the principal transform displacement zone is characterized at the surface by a narrow (<1.5 km) interval of fault scarps in sediment that trends parallel with the transform valley. Extrapolated to the west, this zone links with zones of transform deformation investigated during earlier submersible studies (CYAMEX and Pastouret, 1981). Evidence of low-level hydrothermal discharge was seen at one locality on the EPR axis and at another 8 km west of the axis at the edge of the zone of transform deformation.     

Optimum design of a servo-controlled breathing regulator for underwater divers

This article deals with the analysis, synthesis and performance evaluation of the flow and dynamic characteristics of the new class of servo-controlled breathing regulators for underwater divers.Emphasis on such a class of regulators has been stimulated by their tremendous potential in extending the dive depth and duration because they reduce the diver breathing effort to a great extent. This would eliminate the unnecessary exhaustion of the diver under such extreme diving conditions and accordingly improve his work efficiency underwater.The study aims at developing rational design procedures and guides that would enable the regulator designers to select the optimal design parameters of this class of regulators. Such design guides are based on the analysis of the flow and dynamic characteristics of the servo-controlled regulator as well as the basic principle of the theory of optimum control.The study presents also experimental evaluation techniques of the dynamic characteristics of this class of regulators in order to provide common quantitative means for comparison with the conventional regulator designs.The presented results emphasize the merits and the potential of the servo-controlled regulators as essential tools of supporting the life of divers underwater.     

Semisubmersible response to transient ice forces

The paper presents an analytical and experimental study on the transient response of semisubmersibles to bergybit impact and the strength of bergybit ice to high strain-rate loadings. Two approaches have been proposed for the solution of the semisubmersible-bergybit interaction problem, one using the energy approach and the other using the conventional structural dynamics approach with initial velocity conditions. In addition the local behaviour of the impacted regions have been analysed for deformation and failure. Numerical results have been given for local behaviour of an impacted column and global behaviour of semisubmersible-bergybit system. Experimental study has been reported on the impact strength of iceberg ice at strain rates of 10⁻³, 10⁻² and 10⁻¹; the indentation impact strength of ice is found to be 3–4 times the unaixial compressive strength, at the same strain rate.