钝顶螺旋藻部分原生质体及单细胞的制备与培养

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。     

Serum biochemical and cellular responses to experimental cupric ion challenge in mussels

Our intent has been to develop rapid and sensitive bioassay procedures for laboratory and field applications based on hemolymph factors in animals such as the bay mussel, Mytilus edulis. We found that mussel hemolymph could be easily and repeatedly sampled from individual animals in sufficient amounts to allow analyses for lysozyme, proteolytic activity, total protein and hemocyte counts. In static tests there seemed to be graded responses indicating release of increasing amounts of the above factors into the hemolymph in roughly direct proportion to Cu²⁺ concentration at lethal and sublethal levels. We interpreted this to be evidence for increased lysosomal and cellular disruption as cupric ion exposure was increased or prolonged. At the same time, Cu²⁺ concentrations in the mussel hemolymph increased up to an order of magnitude above ambient in the range of 26 to 267 ppb. We felt that this was evidence for the release of Cu-binding proteins into the mussel hemolymph. Simultaneously with the above events the serum granulocytes increased in numbers above control values by factors of 3 or 4 in response to copper challenge while macrophage (terminology of Moore & Lowe, 1977) counts, including controls, declined precipitously.     

口服免疫药物后中国对虾某些血淋巴因子的测定及方法研究

于１９９１年４－９月，以海捕中国对虾亲虾及养殖中国对虾为材料，运用单向免疫扩散（ＳＲＩＤ）方法测定对虾血淋巴免疫因子，并采用经改进后的Ｈｏｒｏｗｉｔｚ等人的方法测定对虾体内的酚氧化酶（ＰＯ）活性，目的在于运用该两种方法对口服不同免疫药物的养殖对虾的血淋巴免疫因子及ＰＯ活力进行测定。对亲虾和养殖对虾口服不同免疫药物前后的测定结果表明，几种情况下的对虾血淋巴中均存在类似ＩｇＭ的因子，血细胞中存在酚氧化     

In situ tests for ice strength measurements

Ice effects have caused extensive damage. Enormous forces have been recorded. On the other hand, ice has been used as a structural material under various situations. To use ice beneficially and to assess the danger of ice forces, it is necessary to know the mechanical properties of ice.Results from the large number of ice tests carried out in laboratories show the difficulties of sampling and testing procedures. Most of the tests have not reproduced faithfully enough the conditions existing in nature. To obtain adequate results, laborious and slow samplings and preparations of test specimens have been necessary. Recently, extensive use has been made of in situ tests in order to reduce the number of factors whixh can induce errors in measurements. Large scale tests have simulated actual structures. General ice properties have become available from small-scale in situ tests. Since these tests give rapid results, a large number are already available with a good coverage of field conditions.A theoretical basis has been devised for these in situ tests which measure essentially the unconfined and confined compressive strengths of ice, and practical devices have been developed to execute these tests. A correlation of measurements from laboratory tests and in situ tests has established a link between natural and artificial environments. Brittle elastic and clastic theories have been applied, to find the best correlations for ice tests. Test results have been related to the mechanical properties as used in engineering practice.A very summary conclusion of results is that cold sea ice and cold fresh water ice are both very strong. However, depending on the load rate, ice can be quite brittle, which mitigates dangerous effects from ice action against massive structures.     

自主式水下机械手系统研究

本文从水下机械手的总体设计，关节直流电机的驱动和调速，手臂上声纳的水下搜索与跟踪，机械手系统的智能控制和电源等几个方面，介绍了有关自主式水下机械手系统的研究，可为研制自主式水下作业系统提供参考     

Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach

An integrate approach will be discussed for investigations on oxidative stress in xenobiotic toxicity. While the analysis of individual antioxidants is useful for their sensitivity and to understand the mode of action of a stressor, the integration with the analysis of the total antioxidant capacity provides a more holistic assessment of the overall biological significance of such variations. TOSC has a greater predictive value on the health condition of the organisms and allows to discriminate the different role of specific ROS in oxidative stress syndrome.     

High-resolution acoustic mapping of shallow gas in unconsolidated sediments beneath the strait of Georgia,British Columbia

Gassy sediments are detectable acoustically in subbottom profiles of Canada's Fraser Delta slope and prodelta areas. Interstitial gas is typically represented by zones of acoustic turbidity on high-resolution seismic profiles and by gas brightening on air-gun profiles. The top of the acoustically turbid zone is generally highest within the sediment column closest to the river mouths and lies within 10 ms (two-way travel time) of the sea floor in a nearly continuous area that covers over 530 km². Most of the gas is believed to be biogenic in origin, although thermogenic gas derived from underlying Tertiary sedimentary rocks may be present in places.     

Magnetic studies over the northern extension of the Prathap Ridge complex,eastern Arabian Sea

The northern parts of the Prathap and Laccadive Ridge system, eastern Arabian Sea, consist of three parallel basement ridge peaks at varied depths. The topographic highs are associated with either well-developed or subdued magnetic signatures. Model studies, constrained by seismic results, determine the varied nature and depth to the top of the causative basement bodies. Similarities of the geophysical signatures of the ridges and their structural resemblance perhaps point to their common origin. Hence we propose that the Prathap Ridge complex may be a part of the Chagos-Laccadive Ridge system and formed because of the Reunion hotspot activity.