Hemolytic activity and fatty acids composition in the ichthyotoxic dinoflagellate Cochlodinium polykrikoides isolated from Bahía de La Paz, Gulf of California

The hemolytic activity of the dinoflagellate Cochlodinium polykrikoides from Bahía de La Paz, Gulf of California was investigated as part of the ichthyotoxic mechanism of this microalga. Two different kinds of erythrocytes, fish and human, were tested for the hemolytic assay. Since fatty acids have been associated with hemolytic activity in C. polykrikoides, the composition of fatty acids of this dinoflagellate was also analyzed. The concentration of C. polykrikoides causing 50% hemolysis (HE₅₀) was 4.88 and 5.27 × 10⁶ cells L⁻¹, for fish and human erythrocytes, respectively. According to the standard curve of saponin, an equivalence between the hemolytic activity of saponin and the dinoflagellate concentration was found with 1 μg saponin mL⁻¹ equivalent to 1 × 10⁶ cells L⁻¹ of C. polykrikoides. The polyunsaturated fatty acids: hexadecaenoic (16:0), docosahexaenoic (22:6 n3) and octadecapentaenoic (18:5 n3) were found in an abundance of 62% of total fatty acids.     

Surface composition and physical properties of several trans-neptunian objects from the Hapke scattering theory and Shkuratov model

Several different trans-neptunian objects have been studied in order to investigate their physical and chemical properties. New observations in the 1.1-1.4 μm range, obtained with the ISAAC instrument, are presented in order to complete previous observations carried out with FORS1 in the visible and SINFONI in the near infrared. All of the observations have been performed at the ESO/Very Large Telescope. We analyze the spectra of six different objects (2003 AZ₈₃, Echeclus, Ixion, 2002 AW₁₉₇, 1999 DE₉ and 2003 FY₁₂₈) in the 0.45-2.3 μm range with the model of Hapke (Hapke, B. [1981]. J. Geophys. Res. 86, 4571-4586) and the method of Shkuratov et al. (Shkuratov, Y., Starukhina, L., Hoffmann, H., Arnold, G. [1999]. Icarus 137, 235-246). Water ice is found on two objects, and in particular it is confirmed in its amorphous and crystalline states on 2003 AZ₈₄ surface. Upper limits on the water ice content are given for the other four TNOs investigated, confirming previous results (Barkume, K.M., Brown, M.E., Schaller, E.L. [2008]. Astron. J. 135, 55-67; Guilbert, A., Alvarez-Candal, A., Merlin, F., Barucci, M.A., Dumas, C., de Bergh, C., Delsanti, A. [2009]. Icarus 201, 272-283). Whatever the absorption features in the near infrared, all objects but one exhibit a moderate red slope in the visible, as most TNOs and Centaurs. We discuss the implications of the presence of water ice and the probable sources of the red slope.