Bacterioplankton growth and production at the Louisiana hydrocarbon seeps

The growth rate and potential production of bacterioplankton in cold hydrocarbon seeps located along the Louisiana coast were determined using a pulse-labeling technique. Surprisingly, community doubling times are on the order of 1.1 h, which compares to laboratory-grown cultures. We also found that there are differences in growth rates on relatively small geographic scales, suggesting the influence of site-specific geological features (e.g., gas hydrate mounds). Proceeding downslope to deeper waters, methane-oxidizing bacteria appeared to play a more significant role in community productivity. These preliminary experiments indicated, quite unexpectedly, that water column microbes are growing at a more rapid rate than in any other marine system so far studied and that methane may serve as a primary nutrient (carbon) source in these seep-associated microbial assemblages.     

The elemination of re-reflected waves by a porous medium of finite thickness

The elimination of re-reflected waves in a wave channel by installing a porous medium in front of the wavemaker is investigated. The thickness of the porous wall required to eliminate the re-reflected waves is shown to be related to th porosity, friction coefficient, and wave period, as well as to both the positions of the porous medium and the test structure. However, this study indicates that the goal of eliminating re-reflected waves can be achieved by simply varying the thickness of the porous medium according to the wave period, with all the other factors arbitrarily selected.Assuming that the oscillation amplitude of the wavemaker board is constant, the primitive wave amplitude, before reaching the porous medium, becomes smaller as the wave period is increased. In addition, the study found that the required thickness of the porous medium for eliminating the re-reflected wave becomes larger as the wave period is increased. This results in a trend which further reduces the wave amplitude after the wave passes through the porous medium. In consequence, the oscillation amplitude of a wavemaker board has to be adjusted in a larger scale if the wave period is to be increased.     

Environmental overview of Eckernförde Bay,northern Germany

This environmental overview of Eckernförde Bay (northern Germany) summarizes the results of previous studies relevant to the Office of Naval Research's Coastal Benthic Boundary Layer (CBBL) Baltic field exercise conducted during 1993–1994. Significant environmental characteristics include the following: (1) surface sediment distribution is related to water depth, dictated primarily by hydrodynamic reworking of older glacial deposits; (2) the origin and characteristics of small-scale sedimentary structures depend on storm-generated waves and currents; (3) the proximity of the sea surface and sediment —water interface results in a pelagic—benthic coupling that drives biogeochemical processes and produces organicrich, acoustically turbid sediments; and (4) the bay floor is complicated topographically by pockmarks and manmade sedimentary structures.     

Total amount of oceanic excess CO2 taken from the North Pacific subpolar region

Concentrations of total carbonate, alkalinity and dissolved oxygen were obtained near the 1973 GEOSECS stations in the North Pacific subpolar region north of 40°N along 175°E between 1993 and 1994. A difference of excess CO₂ content between the GEOSECS and our expeditions was estimated. The maximum difference in water column inventory of excess CO₂ has increased by about 280 gC m^–2 above 2000 m depth which apparently means an uptake of excess CO₂ taken from air to sea during the last two decades. An averaged value of the annual flux of excess CO₂ at 75–1000 m depth was 8.63±2.01 gC m^–2yr^–1 in the North Pacific subpolar region. By introducing the annual flux of excess CO₂ into a two-box model for the North Pacific subpolar region, a penetration factor of excess CO₂ from air to sea was obtained to be 1.08×10^–2 gC m^–3ppm^–1 in the North Pacific subpolar region. Based on this factor, the surface concentration of excess CO₂ in the North Pacific subpolar region was estimated to be 68 mole I^–1, suggesting that the North Pacific subpolar region absorbed atmospheric excess CO₂ more than the saturated concentration of excess CO₂. Total amount of excess CO₂ taken from the North Pacific subpolar region by 1993 was estimated to be 36.2×10¹⁵ gC, which was equal to about one tenth of that released by human activities after the preindustrial era.     

Isotopic and Elemental Variations of Carbon and Nitrogen in a Mangrove Estuary

Variations in elemental and isotopic ratios of suspended particulate matter (SPM) were investigated in the Guayas River Estuary Ecosystem (GREE) that empties into the Gulf of Guayaquil, Ecuador. Detritus in the system was identified on the basis of extremely high carbon:chlorophyll aratios (>1000). This material had mean δ¹³C of −26·4±0·3, δ¹⁵N of +4·8±0·2, and (C:N)_atomicof 14·1±0·9. The isotopic data were comparable to measurements reported for fresh and degrading mangrove leaves, whereas the elemental ratio was comparatively enriched in nitrogen. Isotope measurements of SPM throughout the GREE were more similar to values for riverine material and detritus compared with that for the coastal end-member. Values indicative ofin situproduced algae, sewage and shrimp pond effluent were only found at selected sites. Bacterial bioassays, which were used to document potential sources of dissolved organic matter in the GREE, were isotopically similar to SPM. This correspondence coupled with the relatively low (C:N)_aof SPM could be explained by bacterial immobolization of nitrogen onto detritus. Finally, tidal variations of (C:N)_aand δ¹³C at a brackish mangrove site were similar in magnitude to spatial variations encountered throughout the GREE. Based on these results, the authors caution that care must be taken when samples are taken for food-web studies in these systems.     

Gonadal development in Japanese medaka (Oryzias latipes) exposed to 17 β-estradiol

The purpose of this research was to evaluate the Japanese medaka (Oryzias latipes) as an ecotoxicological model for the rapid evaluation of environmental estrogens. A novel short-term (48-h) exposure to 17 β-estradiol is proposed in development of a positive control for disruption of gonadal development. Recently hatched medaka fry (30 fry per dose group) with undifferentiated gonads were exposed to 4.0, 29.4, and 115.6 μg/liter of 17 β-estradiol (acetone carrier) for 48 h in a water bath at 25 °C. The fry were then grown-out in spring water for 2 weeks, killed, and processed for histological evaluation. High lethality was encountered during the grow-out period in the 115.6 μg/liter dose group. Fry in the spring water and acetone (carrier) control groups developed into females or males. Fry exposed to 17 β-estradiol developed primarily into females or had testis-ova.     

Seasonal Study of Methane and Nitrous Oxide in the Coastal Waters of the Southern Baltic Sea

Atmospheric and dissolved methane (CH₄) and nitrous oxide (N₂O) were measured in the unique coastal ecosystem of theBoddenwaters, including the western Oder estuary, (southern Baltic Sea) during five campaigns between 1994 and 1997. The CH₄saturations, ranging from 105–15 500%, showed great spatial and temporal variability with maximum values in September and minimum values in December. The N₂O saturations were in the range of 91–312% with a maximum in March. Enhanced concentrations of both gases were observed only in the western Oder estuary near the mouth of the Peene River. Thus, we conclude that the distributions of CH₄and N₂O in the investigatedBoddenwaters are, directly or indirectly, linked to the Peene River runoff and not to the Oder River. Our estimate of the annual CH₄emissions from theBoddenwaters to the atmosphere indicates a significant contribution (c. 17%) to the overall CH₄emissions from the Baltic Sea. In contrast, theBoddenwaters represent only a small source for atmospheric N₂O.CH₄production rates estimated from sediment slurry experiments revealed a significant spatial variability and indicated that methanogenic activity was related to acetate consumption in the surface sediment layer. Sedimentary CH₄production might depend on different amounts of accumulation of organic material.     

Caught in the act: the 20 December 2001 gravity flow event in Monterey Canyon

A sediment gravity flow descended through the axis of Monterey Canyon on 20 December 2001 at 13:35 Pacific standard time. The timing of this event is documented by a current-meter package which recorded an 11.9-dbar pressure increase in less than 10 min and was found 550 m down-canyon from its deployment site, buried completely within a >70-cm-thick gravity flow deposit. This event is believed to have started in less than 290 m of water because an instrument at this location was also lost at the same time. A 178-cm core collected after the event from the axis of the canyon at 1,297-m water depth contained fresh, greenish, chlorophyll-rich organic material at 32-cm sub-bottom depth, suggesting the event extended to this water depth. The only trigger identified for this mass movement event appears to be moderate sea and surf conditions. Thus, gravity flow events of this magnitude do not require an exceptional triggering event.