长石莼(缘管浒苔)(Ulva linza)rbcL全长基因的克隆与序列分析

克隆分离了长石莼(缘管浒苔)光合作用第一关键酶Rubisco大亚基全长基因(rbcL)。首先应用PCR和RT-PCR方法扩增rbcL大片段DNA序列和大片段cDNA序列,结果表明,获得的2个克隆序列完全相同,该rbcL大片段基因不存在内含子。其次应用基因步移方法分别对rbcL基因的5′上游未知序列和3′下游未知序列进行扩增,在此基础上,将3个片段序列进行拼接,获得了rbcL全长基因序列(NCBI登陆号:DQ813497)。序列全长2124bp,其中编码区序列长1425bp,为单外显子基因,编码474个氨基酸;5′非翻译区序列长225bp,转录起始位点为位于翻译起始密码子ATG上游第47个核苷酸G,在距离转录起始位点-9bp—-48bp的范围内有推测的类似原核生物的启动子序列(-10区:TAAAAT、-35区:TTGAAA);3′非翻译区序列长474bp,在距离转译终止密码子TAA下游54—98bp处有一段较长的回文序列,可形成一个22bp的茎结构。密码子偏好性分析结果表明,长石莼(缘管浒苔)rbcL基因偏向使用第三位核苷酸碱基为A和T的密码子。     

珠江黄茅海河口洪季侧向余环流与泥沙输移

2012年洪季对珠江黄茅海河口湾侧向动力结构与泥沙输移过程进行了系统观测,采用动量平衡和泥沙通量机制分解等方法,分析了河口流、温盐和泥沙侧向分布特征以及泥沙输移过程,探讨了侧向动量平衡与泥沙输移机制。洪季黄茅海河口存在明显的侧向流,西滩和北槽均形成表层向东、底层向西的两层侧向流,拦门沙滩顶呈现表、底层向西、中层向东的三层侧向流,而拦门沙前缘侧向流整体向西。河口湾纵向净泥沙通量表现为北槽向海、西滩向陆,拦门沙滩顶及其前缘均向海;侧向净泥沙通量表现为滩顶及其前缘均向西,西滩向东、北槽向西。这种侧向泥沙辐聚过程是高浓度悬沙聚集于滩槽界面的重要原因,向陆净通量是西滩回淤的重要原因。滩槽间侧向余环流动量平衡主要是侧向斜压梯度力、科氏力和侧向平流作用。欧拉平流输运在侧向泥沙输运中起主要作用,潮泵效应也起重要作用。     

Numerical simulation of random wave slamming on structures in the splash zone

The numerical investigation of random wave slamming on superstructures of marine structures in the splash zone is presented in this paper. The impact pressures on the underside of the structure are computed based on the improved volume of fluid method (VOF). The governing equations are Reynolds time-averaged equations and the two equation k– model. The third order upwind difference scheme is applied to the convection term to reduce the effect of numerical viscosity. The numerical wave flume with random wave-maker suitable for VOF is established. Appropriate moving contact-line boundary conditions are introduced to the model wave in contact with and separated from the underside of structure. Parametric studies have been carried out for different incident waves, structure dimensions and structure clearance. The numerical results are verified by the experimental results.     

黄河口及其邻近水域强壮箭虫密度的时空分布及其与环境因子的关系

为探究强壮箭虫（Sagitta crassa）在黄河口及其邻近水域的时空分布特征及其与生物和非生物环境等因子的关系,本文依据2013—2014年在黄河口及其邻近水域6个航次的调查数据,采用丛生指标（I）、聚块性指标（A）和负二项分布参数的倒数（C_A）等聚集指标,分析了强壮箭虫的空间分布特征;应用广义可加模型（GAM）研究了强壮箭虫密度的时空分布和环境因子的关系。结果表明,黄河口及其邻近水域强壮箭虫平均密度为21.85 ind/m3,春、秋季高,冬、夏季低;其密度的空间分布以黄河入海口附近水域为密集中心,向远岸水域逐渐降低;强壮箭虫在黄河口及其邻近水域的聚集特征随密度的变化存在两次聚集和两次扩散;水深、表层水温和浮游桡足类密度是影响其密度时空分布的主要因子,总偏差解释率为55.11%,强壮箭虫密度随水深的增加而减小,随表层水温的升高先增加后减小,随桡足类密度的增加而增加。强壮箭虫密度受黄河口及其邻近水域环境因子变动的影响,具有明显的季节变化和空间分布规律。     

北极快速变化的生态环境响应

北冰洋由于其特殊的地理位置,成为全球变化响应最为敏感的地区。本文聚焦北极海冰变化幅度最大的西北冰洋,从营养盐、叶绿素、浮游植物群落和沉积碳埋藏等变化来讨论海洋生态环境对北极快速变化的响应。尽管太平洋北向流和北极周边河流输入加强可以增加西北冰洋上层营养盐储库,但由于夏季硅藻旺发向沉积物迁出大量生源元素,使得上层营养盐相对亏损,部分海域存在显著的氮限制和硅限制。随海冰减退,尽管夏末海盆区浮游植物呈现小型化趋势,但西北冰洋总体上浮游植物现存量和初级生产力呈现增高的趋势;伴随叶绿素极大层下移、北扩,以硅藻为代表的生物泵过程得以更高效的运转。在沉积物埋藏的有机碳中,除原先北冰洋生态系统占据重要比份的冰藻外,硅藻等藻类的有机碳埋藏也逐渐增加。西北冰洋海洋初级生产力的增加不仅促进了生物泵的运转和碳的埋藏,而且给海洋生态系统提供了更多的食物来源。北极海域目前已成为全球碳源汇格局变化最大、海洋生态系统改变最显著的地区之一。     

基于深海运载器的小型岩芯取样钻机发展现状分析

深海岩芯取样钻机是开展海洋科学研究、深海资源探测和海底地质取样所必备的关键性装备,随着深海运载器的大规模应用,利用运载器优势发展深海运载器小型岩芯取样探测技术得到了国内外学者的关注,并形成了研究热点方向。在深海运载器作业工具应用认识基础上,首次聚焦运载器岩芯取样钻机研究,对国内外运载器岩芯取样钻机的发展现状、技术特点进行了全面综述。同时,基于"蛟龙"号岩芯钻机研制及应用经验,讨论了发展运载器岩芯取样钻机的关键技术。最后总结了后续发展方向,以期为我国深海运载器岩芯钻机的研发和应用提供技术参考。     

基于加权贝叶斯网络的海洋灾害风险评估

全球气候变化背景下,海洋灾害的群发性、难以预见性和灾害链效应日显突出,造成的损失逐年上升,开展海洋灾害的风险评估工作至关重要。针对海洋灾害评估中的不确定问题,本文首先基于风险理论剖析了海洋灾害风险的不确定性特征,构建了灾害评估指标体系;然后基于贝叶斯网络模型,提出针对不确定性灾害评估的风险贝叶斯网络,进而基于主客观定权,构建了加权贝叶斯网络评估模型;最后对我国沿海地区海洋灾害开展评估研究。实验表明,该评估模型有效实现海洋灾害的风险评估,具有实际可操作性。     

Binary Star Fractions from the LAMOST DR4

Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy.     

Solar GLAO and TAO:performance comparisons

Multi-conjugate adaptive optics(MCAO),consisting of several deformable mirrors(DMs),can significantly increase the adaptive optics(AO)correction field of view.Current MCAO can be realized by either star-oriented or layer-oriented approaches.For solar AO,ground-layer adaptive optics(GLAO)can be viewed as an extreme case of layer-oriented MCAO in which the DM is conjugated to the ground,while solar tomography adaptive optics(TAO)that we proposed recently can be viewed as star-oriented MCAO with only one DM.Solar GLAO and TAO use the same hardware as conventional solar AO,and therefore it will be important to see which method can deliver better performance.In this article,we compare the performance of solar GLAO and TAO by using end-to-end numerical simulation software.Numerical simulations of TAO and GLAO with different numbers of guide stars are conducted.Our results show that TAO and GLAO produce the same performance if the DM is conjugated to the ground,but TAO can only generate better performance when the DM is conjugated to the best height.This result has important application in existing one-DM solar AO systems.     

“海上丝绸之路”沿线重要港口竞争力评价

“海上丝绸之路”是促进亚欧和亚非互联互通的重要通道,对其沿线重要港口的竞争力研究,可以为海上丝绸之路的建设提供参考。本文综合考虑港口的自然条件、腹地环境、基础设施、服务水平、政府廉洁度,及其在航运网络中的地位差别等因素,运用熵权-层次分析法对沿线51个国家的99个重要港口的竞争力进行评价。结果表明,① 港口综合竞争力空间分布呈明显的区域化特征,且与国家经济发展水平显著相关,地中海地区港口竞争力普遍较强,亚洲次之,非洲最弱。② 港口的网络地位对竞争力的影响最大,竞争力强的港口多位于重要水域的战略通道,网络地位高,辐射范围广。③ 我国投资的部分沿线港口综合竞争力与成熟港口之间还存在一定差距,但是具备较大的竞争力提升空间。