Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

Interpolation and extrapolation of hydrodynamic coefficients

The solution to the problem of calculating the hydrodynamic forces on multiple floating bodies is summarised. The complex coefficients which characterise the forces at each frequency of oscillation are expensive to compute; interpolation of intermediate values and extrapolation to high frequencies appear difficult since the coefficients seem to vary arbitrarily with frequency. Interpolation and extrapolation schemes based on a novel representation of the coefficients are proposed and tested. The excellent numerical results indicate that the values of the coefficients at a few frequency points can characterise the hydrodynamics at all frequencies. These techniques can save economically significant amounts of computer time and permit investigations which would otherwise be prohibitively expensive.     

An ocean-bottom hydrophone recorder for seismic refraction experiments

A new and inexpensive pop-up ocean-bottom hydrophone recorder has been developed for use in seismic refraction experiments. It is capable of operating in water depths of up to 4000 m and in very rugged topography, and uses an acoustic command system built by the U.K. Institute of Oceanographic Sciences for recovery. The instrument is mounted in an inexpensive cylindrical pressure case based on commercially available extruded aluminium alloy tubing, and uses glass spheres and syntactic foam for buoyancy. Hydrophone and clock signals are frequency modulated and recorded on tape cassettes, with a recording duration of three hours allowing up to 18 programmed shot windows. The prototype has made seven free descents on the Mid-Atlantic ridge and in the Gulf of Oman, and successfully recorded shots under operational conditions for the first time in September 1979. The total component cost of the prototype was £2740 (1979 prices).(Department of Earth Sciences, University of Cambridge Contribution No. ES135).     

Fate and effects of a heavy fuel oil spill on a Georgia salt marsh

Addition of a heavy oil to a Spartina salt marsh in the autumn resulted in high concentrations of polycyclic aromatic hydrocarbons in sediment and benthic animals. The highest concentrations of phenanthrene, chrysene and fluoranthene in the sediment were 112, 105 and 75 ng/g sediment, respectively. These concentrations rapidly decreased during the 20 week period following the spill. The times for these hydrocarbons to decrease to 50% of their highest values, i.e. half-life, were approximately 100, 70 and 30 days in sediment, mussels and oysters, respectively. Benthic macrofauna species showed three responses to oil addition which included no change, an increase, or a decrease in the population. No changes were noted in populations of fiddler crabs (Uca pugnax), oysters (Crassostrea virginica), and mussels (Modiolus demissus). Mud snails (Nassarius obsoleta) increased in density after the spill due to immigration of adult snails from untreated areas to scavenge on animals killed by the oil. Many of the adult periwinkles (Littorina irrorata) were killed by the oil. In the spring, juvenile periwinkles recolonised to oiled areas as a result of larvae settling.     

Carbon dioxide partial pressures in North Pacific surface waters — Time variations

Observations were made of time variations of the carbon dioxide partial pressures (Pco₂) of the atmosphere and surface sea waters in the Pacific subarctic region. Data were obtained on a cruise of the USC & GSSSURVEYOR in October, 1968 and on the TRANSPAC expedition of the CNAVENDEAVOUR in March–April, 1969. A rise in surface water Pco₂ of 18×10^–6 atm occurred in a period of 30–45 days in March–April due principally to spring warming of surface waters. An average increase of 60×10^–6 atm occurred between October, 1968 and March, 1969 as a result mainly of cessation of summer phytoplankton production and the onset of winter-storm-driven vertical mixing. Because the air-sea Pco₂ gradient not only changed appreciably in magnitude but also changed sign, there are important implications for calculations of air-sea exchange of carbon dioxide on the ocean wide scale.Data contained in this paper comprise part of a dissertation to be submitted by Louis I. Gordon in partial fulfillment of the requirements for the Ph. D. at Oregon State University.     

Effect of agricultural activity on levels of organochlorine pesticides in hard corals, fish and molluscs from the Great Barrier Reef

An organochlorine baseline study of selected corals, fish and molluscs has established that lindane is the only consistently detectable persistent pesticide in Great Barrier Reef organisms sampled. Samples taken during 1976–1977 from twenty reefs between Heron Island and Lizard Island provide evidence from the distribution of lindane that the contamination is related to agricultural usage in the coastal sugar cane growing regions. Consideration is given to the possible mode of transport of lindane to the reef environment. No polychlorinated biphenyls were detected in any of the samples and only scattered samples contained levels of residues other than lindane. Inner marginal shelf reefs of the Great Barrier Reef may be the most pristine environment yet reported for organochlorine pollutants.     

Assessment of imposex in the common whelk, Buccinum undatum (L.) from the Eastern Scheldt, The Netherlands

Several cruises were carried out on the Eastern Scheldt between September 1992 and March 1995 to study the frequency and appearance of imposex in the common whelk, Buccinum undatum L. Incidences of imposex were always > 90%. For the first time several stages of imposex in Buccinum undatum are reported. The evolution of imposex in the whelk seems to follow a similar route as in Hinia reticulata belonging to the same superfamily (Buccinacea). Tissue organotin concentrations of animals collected in February 1995 showed the highest organotin concentrations in the digestive gland and lowest in the foot of the animals. Concentrations of phenyltin compounds (up to 625 ngSn/g DW) were much higher than those of butyltin compounds (up to 40 ngSn/g DW).     

Solubility of hydrous ferric oxide and iron speciation in seawater

Iron solubility equilibria were investigated in seawater at 36.22‰ salinity and 25°C using several filtration and dialysis techniques. In simple filtration experiments with 0.05 μm filters and Millipore ultra-filters, ferric chlorides fluorides, sulfates, and FeOH²⁺ species were found to be insignificant relative to Fe(OH)₂⁺ at p[H⁺] = ?log [H⁺] greater than 6.0. Hydrous ferric oxide freshly precipitated from seawater yielded a solubility product of ${}^1\text{K}{}_{\mathrm{<mtext>so</mtext>}}\text{=}\text{[Fe}{}^{\mathrm{3+}}\text{][H}{}^{\mathrm{+}}\text{]}{}^{\mathrm{?3}}\text{= 4.7 · 10}{}^5$. Solubility studies based on the rates of dialysis of various seawater solutions and on the filtration of acidified seawater solutions indicated the existence of the Fe(OH)₃⁰ species. The formation constant for this species can be calculated as ${}^1\text{β}{}_3\text{=}\text{[Fe(OH)}{}_3{}^0\text{] [H}{}^{\mathrm{+}}\text{]}{}^3\text{/[Fe}{}^{\mathrm{3+}}\text{] = 2.4 · 10}{}^{\mathrm{?14}}$. The Fe(OH)₄^? species is present at concentrations which are negligible compared to Fe(OH)₂⁺ and Fe(OH)₃⁰ in the normal pH range of seawater. However, there is at least one other significant ferric complex in seawater above p[H⁺] = 8.0 (possibly with bicarbonate, carbonate, or borate ions) in addition to the Fe(OH)₂⁺ and Fe(OH)₃⁰ species.