Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

Wind-driven interannual variability over the northeast Pacific Ocean

Interannual variability of the sea surface height (SSH) over the northeast Pacific Ocean is hindcast with a reduced-gravity, quasi-geostrophic model that includes linear damping. The model is forced with monthly Ekman pumping fields derived from the NCEP reanalysis wind stresses. The numerical solution is compared with SSH observations derived from satellite altimeter data and gridded at a lateral resolution of 1 degree. Provided that the reduced gravity parameter is chosen appropriately, the results demonstrate that the model has significant hindcast skill over interior regions of the basin, away from continental boundaries. A damping time scale of 2 to 3 years is close to optimal, although the hindcast skill is not strongly dependent on this parameter.A simplification of the quasi-geostrophic model is considered in which Rossby waves are eliminated, yielding a Markov model driven by local Ekman pumping. The results approximately reproduce the hindcast skill of the more complete quasi-geostrophic model and indicate that the interannual SSH variability is dominated by the local response to wind forcing. There is a close correspondence the two leading empirical orthogonal modes of the local model and those of the observed SSH anomalies. The latter account for over half of the variance of the interannual signal over the region.     

Dominant amphipods of Posidonia oceanica seagrass meadows display considerable trophic diversity

Gut content examination and trophic markers (fatty acids, stable isotopes of C and N) were combined to delineate the diet of the dominant species of amphipods from Mediterranean Posidonia oceanica seagrass meadows and to highlight trophic diversity among this community. Our results indicate that, although all dominant species heavily relied on macroalgal epiphytes, considerable interspecific dietary differences existed. Carbon stable isotope ratios notably showed that some of the amphipod species favored grazing on epiphytes from leaves or litter fragments (Apherusa chiereghinii, Aora spinicornis, Gammarus aequicauda), while others such as Dexamine spiniventris preferred epiphytes from rhizomes. The remaining amphipods (Caprella acanthifera, Ampithoe helleri and Gammarella fucicola) readily consumed both groups. In addition, SIAR modeling suggested that most species had a mixed diet, and relied on several food items. Fatty acid analysis and gut contents revealed that contributions of microepiphytic diatoms and of benthic and suspended particulate organic matter to the diet of amphipods were anecdotal. None of the examined species seemed to graze on their seagrass host [low 18:2(n‐6) and 18:3(n‐3) fatty acids contents], but Gammarus aequicauda partly relied on seagrass leaf detritus, as demonstrated by the lesser ¹³C‐depletion of their tissues. Overall, our findings suggest that amphipods, because of their importance in the transfer of organic matter from primary producers and detritus to higher rank consumers, are key items in P. oceanica‐associated food webs.     

A regime shift in sediment export from a coastal watershed during a record wet winter,California: Implications for landscape response to hydroclimatic extremes

Small, steep watersheds are prolific sediment sources from which sediment flux is highly sensitive to climatic changes. Storm intensity and frequency are widely expected to increase during the 21st century, and so assessing the response of small, steep watersheds to extreme rainfall is essential to understanding landscape response to climate change. During record winter rainfall in 2016–2017, the San Lorenzo River, coastal California, had nine flow peaks representing 2–10‐year flood magnitudes. By the third flood, fluvial suspended sediment showed a regime shift to greater and coarser sediment supply, coincident with numerous landslides in the watershed. Even with no singular catastrophic flood, these flows exported more than half as much sediment as had a 100‐year flood 35 years earlier, substantially enlarging the nearshore delta. Annual sediment load in 2017 was an order of magnitude greater than during an average‐rainfall year, and 500‐fold greater than in a recent drought. These anomalous sediment inputs are critical to the coastal littoral system, delivering enough sediment, sometimes over only a few days, to maintain beaches for several years. Future projections of megadroughts punctuated by major atmospheric‐river storm activity suggest that interannual sediment‐yield variations will become more extreme than today in the western USA, with potential consequences for coastal management, ecosystems, and water‐storage capacity. The occurrence of two years with major sediment export over the past 35 years that were not associated with extremes of the El Niño Southern Oscillation or Pacific Decadal Oscillation suggests caution in interpreting climatic signals from marine sedimentary deposits derived from small, steep, coastal watersheds, to avoid misinterpreting the frequencies of those cycles. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.     

Simulating Stable Isotope Ratios in Plumes of Groundwater Pollutants with BIOSCREEN‐AT‐ISO

BIOSCREEN is a well‐known simple tool for evaluating the transport of dissolved contaminants in groundwater, ideal for rapid screening and teaching. This work extends the BIOSCREEN model for the calculation of stable isotope ratios in contaminants. A three‐dimensional exact solution of the reactive transport from a patch source, accounting for fractionation by first‐order decay and/or sorption, is used. The results match those from a previously published isotope model but are much simpler to obtain. Two different isotopes may be computed, and dual isotope plots can be viewed. The dual isotope assessment is a rapidly emerging new approach for identifying process mechanisms in aquifers. Furthermore, deviations of isotope ratios at specific reactive positions with respect to “bulk” ratios in the whole compound can be simulated. This model is named BIOSCREEN‐AT‐ISO and will be downloadable from the journal homepage.     

Tyre track pools and puddles – Anthropogenic contributors to aquatic biodiversity

Twelve sites of tyre track pools and puddles situated in woodland, heath and pasture in Dorset UK were examined to determine their macroinvertebrate species richness and community changes over the course of one year.174 taxa were found with Diptera (59) and Coleoptera (48) contributing 61% of the total. The most frequently occurring and ubiquitous groups were nematoceran dipterans, Oligochaeta, Coleoptera, Crustacea and Lamellibranchiata.Species richness varied with season and on average was highest in March and November samples. On average only 26% (range 16–40%) of the combined total number of taxa found in spring (March) and autumn (November) samples from a site were also found there in each of these seasons individually, indicating a high species turnover through the year.The tyre track pools contributed to local aquatic biodiversity by adding 29 taxa to previously published taxa lists from aquatic habitats in the area. The relative richness of the tyre track pools is attributed to their successional variation in a heterogeneous landscape.Conservation value of 9 of the 12 sites was rated Very high to High and nine regionally notable or rare taxa were recorded. It is suggested that the important conservation status of the tyre track pools warrants greater recognition and further intensive study.     

Bio‐geomorphic effects on tidal channel evolution: impact of vegetation establishment and tidal prism change

The long‐term (10–100 years) evolution of tidal channels is generally considered to interact with the bio‐geomorphic evolution of the surrounding intertidal platform. Here we studied how the geometric properties of tidal channels (channel drainage density and channel width) change as (1) vegetation establishes on an initially bare intertidal platform and (2) sediment accretion on the intertidal platform leads to a reduction in the tidal prism (i.e. water volume that during a tidal cycle floods to and drains back from the intertidal platform). Based on a time series of aerial photographs and digital elevation models, we derived the channel geometric properties at different time steps during the evolution from an initially low‐elevated bare tidal flat towards a high‐elevated vegetated marsh. We found that vegetation establishment causes a marked increase in channel drainage density. This is explained as the friction exerted by patches of pioneer vegetation concentrates the flow in between the vegetation patches and promotes there the erosion of channels. Once vegetation has established, continued sediment accretion and tidal prism reduction do not result in significant further changes in channel drainage density and in channel widths. We hypothesize that this is explained by a partitioning of the tidal flow between concentrated channel flow, as long as the vegetation is not submerged, and more homogeneous sheet flow as the vegetation is deeply submerged. Hence, a reduction of the tidal prism due to sediment accretion on the intertidal platform, reduces especially the volume of sheet flow (which does not affect channel geometry), while the concentrated channel flow (i.e. the landscape forming volume of water) is not much affected by the tidal prism reduction. Copyright © 2012 John Wiley & Sons, Ltd.     

Temporal Pointing Variations of the Solar Dynamics Observatory’s HMI and AIA Instruments on Subweekly Time Scales

Achieving subarcsecond co-registration across varying time-lines of multi-wavelength and instrument images is difficult and requires an accurate characterization of the instrument pointing jitter. We investigated the internal pointing errors on daily and yearly time-scales that occur across the Solar Dynamics Observatory’s (SDO) Atmospheric Imaging Assembly (AIA) and Helioseismic Magnetic Imager (HMI). Using cross-correlation techniques on the AIA 1700 Å passband and the HMI line-of-sight magnetograms from three years of observational image pairs at approximately three-day intervals, internal pointing errors were quantified. Pointing variations of ±?0.26″ (jitter-limited) and ±?0.50″ in the solar East–West (x) and North–South (y) directions, respectively, were measured. AIA observations of the Venus transit in June 2012 were used to measure existing coalignment offsets in all passbands. We found that the AIA passband pointing variations are 〈ΔX _CO〉=1.10″±1.41″ and 〈ΔY _CO〉=1.25″±1.24″ when aligned to the HMI nominal image center, referred to here as the CutOut technique. Minimal long-term pointing variations found between limb and correlation derived pointings provide evidence that the image-center positions provided by the instrument teams achieve single-pixel accuracy on time scales shorter than their characterization. However, daily AIA passband pointing variations of ??1.18″ indicate that autonomous subarcsecond co-registration is not fully achieved yet.     

The fusion crust of the Winchcombe meteorite: A preserved record of atmospheric entry processes

Fusion crusts form during the atmospheric entry heating of meteorites and preserve a record of the conditions that occurred during deceleration in the atmosphere. The fusion crust of the Winchcombe meteorite closely resembles that of other stony meteorites, and in particular CM2 chondrites, since it is dominated by olivine phenocrysts set in a glassy mesostasis with magnetite, and is highly vesicular. Dehydration cracks are unusually abundant in Winchcombe. Failure of this weak layer is an additional ablation mechanism to produce large numbers of particles during deceleration, consistent with the observation of pulses of plasma in videos of the Winchcombe fireball. Calving events might provide an observable phenomenon related to meteorites that are particularly susceptible to dehydration. Oscillatory zoning is observed within olivine phenocrysts in the fusion crust, in contrast to other meteorites, perhaps owing to temperature fluctuations resulting from calving events. Magnetite monolayers are found in the crust, and have also not been previously reported, and form discontinuous strata. These features grade into magnetite rims formed on the external surface of the crust and suggest the trapping of surface magnetite by collapse of melt. Magnetite monolayers may be a feature of meteorites that undergo significant degassing. Silicate warts with dendritic textures were observed and are suggested to be droplets ablated from another stone in the shower. They, therefore, represent the first evidence for intershower transfer of ablation materials and are consistent with the other evidence in the Winchcombe meteorite for unusually intense gas loss and ablation, despite its low entry velocity.