Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

The final phases of the Viking mission to Mars

Of the four spacecraft that the Viking Project put into operation at Mars in the summer of 1976, one continues to acquire data periodically. The missions of the two orbiters were terminated by the depletion of their attitude-control gas: Orbiter 2 in July 1978 and Orbiter 1 in August 1980. Lander 2 was shut down in April 1980 because of degradation of its batteries. Lander 1 is programmed to continue acquiring a modest number of imaging, meteorology, and ranging data periodically until December 1994. During its final year Orbiter 1 continued to produce excellent data from its full complement of instruments—two cameras, two infrared instruments (thermal mapper and water vapor detector), and the radio subsystem. The major emphasis was on photography, with 10,000 images being acquired. These included two very large swaths of high-resolution contiguous coverage of the Martian surface and the completion of the moderate-resolution mapping of nearly the entire surface, as well as miscellaneous other observations. The majority of these images has not been processed and examined, but the others have revealed many previously unobserved features and have greatly enhanced the base for a geological understanding of the planet. The history of Viking mission operations is brought up to date.     

Evolution of a complex isolated dome system,Cerro Pizarro,central México

Cerro Pizarro is an isolated rhyolitic dome in the intermontane Serdán-Oriental basin, located in the eastern Trans-Mexican Volcanic Belt. Cerro Pizarro erupted ~1.1 km³ of magma at about 220 ka. Activity of Cerro Pizarro started with vent-clearing explosions at some depth; the resultant deposits contain clasts of local basement rocks, including Cretaceous limestone, ~0.46-Ma welded tuff, and basaltic lava. Subsequent explosive eruptions during earliest dome growth produced an alternating sequence of surge and fallout layers from an inferred small dome. As the dome grew both vertically and laterally, it developed an external glassy carapace due to rapid chilling. Instability of the dome during emplacement caused the partial gravitational collapse of its flanks producing various block-and-ash-flow deposits. After a brief period of repose, re-injection of magma caused formation of a cryptodome with pronounced deformation of the vitrophyric dome and the underlying units to orientations as steep as near vertical. This stage began apparently as a gas-poor eruption and no explosive phases accompanied the emplacement of the cryptodome. Soon after emplacement of the cryptodome, however, the western flank of the edifice catastrophically collapsed, causing a debris avalanche. A hiatus in eruptive activity was marked by erosion of the cone and emplacement of ignimbrite derived from a caldera to the north of Cerro Pizarro. The final growth of the dome growth produced its present shape; this growth was accompanied by multiple eruptions producing surge and fallout deposits that mantle the topography around Cerro Pizarro. The evolution of the Cerro Pizarro dome holds aspects in common with classic dome models and with larger stratovolcano systems. We suggest that models that predict a simple evolution for domes fail to account for possibilities in evolutionary paths. Specifically, the formation of a cryptodome in the early stages of dome formation may be far more common than generally recognized. Likewise, sector collapse of a dome, although apparently rare, is a potential hazard that must be recognized and for which planning must be done.Editorial responsibility: J. Gilbert     

Eminent domain destroys a community: leveling East Arlington to make way for the Pentagon

This study examines the eminent domain action in 1942 where the federal government took 411 acres of land, including an African American neighborhood, to construct the Pentagon building in Arlington County, Virginia. The Fifth Amendment of the U.S. Constitution acknowledges the right of the federal government to exercise eminent domain where needed for the common good, but requires that property owners be given just compensation. This study explores the issue of compensation: what the residents lost when they were forced to move, how that loss was compensated, and whether the compensation was “just.” The study data include interviews with former residents of the neighborhood, oral histories, land records, census data, photographs, and newspaper articles. The study concludes that the federal government compensated the residents for land and buildings, but was unable to compensate for a lost community. For many residents, that community is mourned even 72 years later.     

The Discovery of the Chemical Composition - Kinematics Connection in the 1950's

This paper reviews the recognition that kinematic characteristics of stars are related to their spectroscopic appearance on low and intermediate dispersion spectra. These differences result from the differences in the abundances of metals as determined from high dispersion spectra. Presumably, these abundance differences reflect differences in age. The high-velocity carbon stars share the molecular peculiarities of the high-velocity oxygen giants but, because of the greater strength of the bands of carbon-containing molecules, exhibit them to a greater degree. This revised version was published online in July 2006 with corrections to the Cover Date.     

Changes in beach water table elevation during neap and spring tides on a sandy estuarine beach, Delaware Bay, New Jersey

A field investigation of temporal and spatial changes in wind and wave characteristics, runup and beach water table elevation was conducted on the foreshore of an estuarine beach in Delaware Bay during neap (April 9, 1995) and spring (April 16, 1995) tides under low wave-energy conditions. The beach has a relatively steep, sandy foreshore and semi-diurnal tides with a mean range of 1.6 m and a mean spring range of 1.9 m. Data from a pressure transducer placed on the low tide terrace reveal a rate of rise and fall of the water level on April 16 of 0.09 mm s⁻¹ resulting in a steeper tidal curve than the neap tide on April 9. Data from three pressure transducers placed in wells in the intertidal foreshore reveal that the landward slope of the water table during the rising neap tide was lower than the slope during spring tide, and there was a slower rate of fall of the beach water table relative to the fall of the tide. Wave heights were lower on April 9 (significant height from 17.1 min records <0.16 m). The water table elevation was 0.08 m higher than the water in the bay at the time of high water, when maximum runup elevation was 0.29 m above high water and maximum runup width was 2.0 m. The elevation of the water table was 0.13 m higher than the maximum elevation of water level in the bay 74 min after high water, when wave height was 0.12 m and wave period was 2.7 s. The use of mean bay water level at high tide will underpredict the elevation of the water table in the beach, and demarcation of biological sampling stations across the intertidal profile based on mean tide conditions will not accurately reflect the water content of the sandy beach matrix.     

Field application at a DNAPL-contaminated site in Nanjing and discussion of a source search algorithm based on stochastic modeling and Kalman filter

Delineation of contaminant sources is vital for successful groundwater and soil remediation. With reliable source information, remediation time and cost can be dramatically reduced. An optimal contamination source search strategy incorporating Monte Carlo method, Kalman filtering and fuzzy set theory was applied to a contaminated site in Nanjing to define suspected multiple DNAPL source locations. Using the available nine sample data, the algorithm identified sources #1 and #4 as true sources, and sources #5 and #6 as false ones. The algorithm results for sources #2 and #3 were inconclusive. Three numerical experiments based on specific site conditions were then designed and conducted to determine the influencing factors on the algorithm’s convergence in sources #2 and #3. The numerical experiments tested the effect of multiple sources, the effect of the sources location in relation to the groundwater flow direction and the effect of a low permeability field on the convergence of the algorithm. Based on the numerical experiments and an understanding of the manufacturing site operations, sources #2 and #3 are likely to be true sources. Their moderate weights have been stabilized due to the existence of multiple true sources and the scarcity of informative sampling data, caused by the low permeability field. The moderate weight value of source #3 also includes a contribution from an overlapping plume caused by the sources’ parallel-to-flow layout pattern. It can be concluded that the algorithm works best for high permeability sites where potential source locations are scattered and source location patterns are orthogonal to the groundwater flow.     

Non-Energy Resources,Connecticut Coastal Waters,Year 9 and 10 Activities

The Connecticut effort in year 9 of the Continental Margins Program concentrated on a 13.6-mi2 area south of the Housatonic River. This area was chosen for a side-scan sonar survey because it lies on and adjacent to the bathymetric expressions of two fairly large, subcropping, potential sources of coarse material. Previous seismic work in the area indicated that outcrops or subcrops of these potential source deposits could be delineated using their bathymetric expression. Owing to the limited resolution of the seismic data, a correlation between bottom type and underlying source deposits could not be made with the seismics alone. Results from the November 1993 side-scan survey show that although the source deposits have discernible bathymetric expressions, they are not cropping out as much as expected. As a result, bottom type is not necessarily determined by subcropping deposits in this particular area. Year 10 work was concentrated in Fishers Island Sound, where three areas of potential interest for near-shore gravel resources had previously been identified. These areas were surveyed, during the spring of 1996, using the RoxAnn Seabed Classification System. A small video camera and a Van Veen grab sampler were used to calibrate and verify the RoxAnn data. Although previous sampling had indicated the presence of gravel or gravelly sediment in all of the survey areas, the RoxAnn results showed less gravel than anticipated. Vibrant eelgrass beds and other habitat indicators were detected in all of the survey areas. Given the variable sediment results, and the high habitat potential of the areas surveyed, the likelihood of developing a sand and gravel supply from the near shore of Fishers Island Sound appears quite low.