Comparative toxicities of chlorinated biphenyls on sea urchin egg fertilisation and embryogenesis

The aim of this study was to compare the biological actions of selected chlorinated biphenyls (CB's) (with different degrees of chlorine substitution), using the sea urchin test system, for their ability to affect fertilisation, embryogenesis and mitotic activity. Sea urchin embryos were exposed to the test agents throughout larval development or for shorter intervals (e.g. before or after hatching). Sperm or eggs were exposed before fertilisation, thereafter the contaminants were removed from embryo cultures. The results showed maximal activities from medium-chlorinated (tetra- and hexachloro-substituted) compounds, as compared to low- and high-chlorinated biphenyls (mono-, di- and octachloro-substituted), at nominal levels ranging from 10⁻⁶m to 10⁻⁵m. This held true for the different parameters and treatment schedules. Developmental defects were only detected following post hatching exposure of embryos to CB's, whereas pre-hatching exposure failed to reveal any embryological or cytogenetic abnormalities.     

An analysis of 3.5 kHz acoustic reflections and sediment physical properties

Near-bottom normal incidence acoustic reflection data and sediment physical property data are used to study the relationships between acoustic reflections and sediment physical properties. A pinger-hydrophone experiment was performed to obtain the necessary acoustic reflection data. In addition, a standard piston core was retrieved in the acoustic survey area for physical property analysis. The piston core was sampled and 13 properties were measured at 55 locations within the top 12 m of the core. Correlation studies amongst the sediment physical properties resulted in the following strong correlations: acoustic impedance ($\text{Z}$) and porosity ($\text{N}$), (0.96); water content ($\text{WC}$) and $\text{Z}$, (0.95); bulk density ($\text{BD}$) and $\text{Z}$, (0.99).The empirical orthonormal function (EOF) method was employed for acoustic signal analysis. This method assumes no a-priori models of the sediment or causality. The EOF method reduced the acoustic data to 8 functions that contained 97.6% of the sample variance. The EOFs were subsequently analysed by using cepstrum analysis which reveals time delay information and enhances detecting zones of reflectivity. The result of the sediment physical property and cepstrum analysis indicates that zones of reflectivity are essentially zones of relatively high acoustic impedance, low porosity, and low phi (high mean grain size).     

The Ectoparasitic Gastropod Boonea (= Odostomia) impressa: Population Ecology and the Influence of Parasitism on Oyster Growth Rates

Abstract. Boonea (= Odostomia) impressa is a common ectoparasite of oysters. In the laboratory, small oysters (Crassostrea virginica) parasitized by natural densities of B. impressa produced 75 % less new shell than unparasitized oysters. Shell deposition rates of previously parasitized oysters increased significantly after all B. impressa were removed. Thus, the decrease in growth rate, although significant, apparently was not permanent. B. impressa preferentially parasitized small, living oysters (≤2.5cm) in the field, even though a higher percentage of large, living oysters (>2.5cm) was available. The snails maintained an aggregated distribution on the oyster reef. The number of B. impressa per oyster clump was positively correlated with the number of living oysters per clump, however some clumps with few or no living oysters had many B. impressa. Thus, food availability only partially explained the pattern of distribution. B. impressa was very mobile. About 50 % of the population moved in one week. Reproduction occurred throughout the year with a peak period in May. Recruitment was greatest in July, however new recruits were observed throughout the year. The reduction in growth rate of parasitized oysters, the snaiľs propensity towards parasitizing small oysters and the snail's tendency to be contagiously distributed suggests that B. impressa potentially exerts a significant influence on the population structure and health of oyster populations.     

Wave forces on submarine pipelines near a plane boundary

Forces induced by regular waves on submarine pipelines resting on as well as near a plane boundary and aligned parallel to wave fronts of the oncoming waves are investigated experimentally. The inline hydrodynamic coefficients of drag and inertia are evaluated through the use of Morison equation and the least squares method. The transverse force is analysed in terms of maximum transverse force and transverse root mean square (r.m.s.) coefficients. The resulting inline and transverse hydrodynamic coefficients are correlated with the period parameter or Keulegan-Carpenter number and relative clearance of the pipeline from the plane boundary. The effect of depth parameter on these coefficients and the correlation between maximum transverse force and transverse r.m.s. coefficients are also reported.     

Study of the fauna inhabiting the holdfasts of Laminaria hyperborea (gunn.) fosl. along some environmental and geographical gradients

A survey of the holdfast fauna of Laminaria hyperborea was carried out at thirty-five sites around the UK, mostly along the North Sea and south coasts.Data from different age classes of holdfast from a polluted and unpolluted site indicate that volume of holdfast space is a most important measurement of sample size, a minimum size of sample being one litre and, preferably, three.Gradients of increased heavy metals were defined down the North Sea and west coasts and, by utilising measurements of kelp penetration, water clarity gradients were determined as increasing northwards on the west coast and both north and southwards on the North Sea coast from a point near Tyne and Wear county. Analysis of holdfast fauna showed numerous gradients of total numbers, species and diversity which correlate closely with the heavy metal and water clarity gradients, or with undetermined parameters analogous to latitude and longitude. Where there were increases of heavy metals or decreases of water clarity, various reciprocal relationships between suspension feeders and all other trophic groups were commonly observed. In the North Sea numbers of suspension feeders increased while numbers of all other trophic groups declined. On the west coast a heavy metal increase correlated with a decreasing species richness of all trophic categories except suspension feeders, while with total numbers only suspension feeders showed an increase. On the south coast transect neither gradient existed, and only here did all trophic categories covary, except at one very polluted site where the same reciprocal relationship was again indicated.In terms of species similarity the North Sea showed a greater pool of common species than occurred between sites of other regions and between North Sea sites with other regions, despite the widest range of heavy metal levels and wate clarity that existed there. Between-site similarity appeared to be much more dependent on geographical proximity than on the two measured environmental factors. It is suggested that effects of clarity-reduction can be measured by trophic analysis more successfully than by species presence or absence, and that changes of holdfast fauna along the North Sea coast are due largely to pollution.     

Concentrations of suspended matter and particulate cadmium,copper, lead and zinc in the Indian sector of the antarctic ocean

The concentrations of suspended matter and particulate Cd, Cu, Pb and Zn were determined for 36 samples collected at 6 stations in the Antarctic Ocean during December, 1970 and January, 1971 using membrane filters. The concentration of suspended matter was determined gravimetrically and trace metal levels were determined using anodic stripping voltammetry. For waters deeper than 100 m the concentration of suspended matter was < 100 μg l^?1. Concentrations up to 542 μg l^?1 were recorded between surface and 100 m. Individual concentrations of the metals were scattered with depth. Average concentrations of particulate metals were: Cd, 3.5 ng l^?1; Cu, 100 ng l^?1; Pb, 35 ng l^?1; and Zn, 230 ng ;l^?1 These measurements represent non-steady state conditions of early Antarctic summer as the ice pack disintegrates and biological activity increases.     

Effect of major ion variations in the marine environment on the specific gravity-conductivity-chlorinity-salinity relationship

Partial equivalent conductances and partial equivalent volumes of the major constituents in seawater were used to evaluate the specific gravity-conductivity-chlorinity-salinity relationships in the marine environment. For example, in the open ocean, the relationships between Cl‰ and both S‰ and specific gravity are valid to within 0.014‰ and 0.014 σ_t, respectively. The relationships between conductivity and S‰ and specific gravity are valid to within 0.006‰ and 0.007 σ_t. In river diluted nearshore areas specific gravity anomalies inferred from Cl‰, can be as great as 0.06 σ_t and 0.04 σ_t when inferred from a conductivity ratio measurement.     

JONSWAP's parameters: Sorting out the inconsistencies

The original prognostic equations for the JONSWAP-spectrum contained inconsistencies. A subsequent paper (Hasselmann et al., 1976, J. phys. Oceanogr.6, 200–208) attempted to regularise the situation. This paper shows that there were still inconsistencies in the prognostic equations giving overestimations of the first moment of the spectrum and consequently the significant wave height. The prognostic equations are reworked systematically and results presented. It is shown that variable σ_a, σ_b and γ must be used to achieve consistent results     

Cyclone and bioturbation effects on sediments from coral reef lagoons

Despite the severity of tropical cyclone ‘Winifred’, which crossed the Great Barrier Reef on 1 February 1986, there were little long-term effects on lagoon surface sediments from reefs in its path. Short-term effects were apparent only at one particularly exposed area. These were: an increase in proportion of the coarse fraction, the establishment of sand ripples, and the destruction of the mounds produced by callianassid shrimps (normally the dominant topographic feature). Within six weeks this area was indistinguishable from a typical reef lagoon. This is probably the result of sediment reworking by callianassid shrimp, involving selective burial of the coarse fragments and transport to the surface of finer particles. Sediment turnover rates by callianassids are commensurate with change to the sediment within the relatively short period observed. The sediment fauna responded quickly to the changes in sediment type. Immediately after the cyclone the disturbed area supported a fauna typical of the coarse sediments on the shallow reef flat, as the sediment reverted to a more normal type so the fauna changed back to that typical of a reef lagoon.     

Thickening of the oceanic layer in the Pacific Ocean

A statistical analysis of published seismic refraction results in the Pacific Ocean confirms the existence of a progressive thickening of the oceanic layer with age, following an inverse exponential law. There is no strong indication in the Pacific data of an increase in the thickness of layer 2 with decreasing spreading rate.Contribution No. 93 of the Département Scientifique of the Centre Océanologique de Bretagne, B.P. 337, 29200, Brest, France.