Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.     

Wave data assimilation using a hybrid approach in the Persian Gulf

The main goal of this study is to develop an efficient approach for the assimilation of the hindcasted wave parameters in the Persian Gulf. Hence, the third generation SWAN model was employed for wave modeling forced by the 6-h ECMWF wind data with a resolution of 0.5°. In situ wave measurements at two stations were utilized to evaluate the assimilation approaches. It was found that since the model errors are not the same for wave height and period, adaptation of model parameter does not result in simultaneous and comprehensive improvement of them. Therefore, an approach based on the error prediction and updating of output variables was employed to modify wave height and period. In this approach, artificial neural networks (ANNs) were used to estimate the deviations between the simulated and measured wave parameters. The results showed that updating of output variables leads to significant improvement in a wide range of the predicted wave characteristics. It was revealed that the best input parameters for error prediction networks are mean wind speed, mean wind direction, wind duration, and the wave parameters. In addition, combination of the ANN estimated error with numerically modeled wave parameters leads to further improvement in the predicted wave parameters in contrast to direct estimation of the parameters by ANN.     

Transient seepage analysis in zoned anisotropic soils based on the scaled boundary finite‐element method

The scaled boundary finite‐element method, a semi‐analytical computational scheme primarily developed for dynamic stiffness of unbounded domains, is applied to the analysis of unsteady seepage flow problems. This method is based on the finite‐element technology and gains the advantages of the boundary element method as well. Only boundary of the domain is discretized, no fundamental solution is required and singularity problems can be modeled rigorously. Anisotropic and non‐homogeneous materials satisfying similarity are modeled with no additional efforts. In this study, firstly, formulation of the method for the transient seepage flow problems is derived followed by its solution procedures. The accuracy, simplicity and applicability of the method are demonstrated via four numerical examples of transient seepage flow – three of them are available in the literature. Homogenous, non‐homogenous, isotropic and anisotropic material properties are considered to show the versatility of the technique. Excellent agreement with the finite‐element method is observed. The method out‐performs the finite‐element method in modeling singularity points. Copyright © 2014 John Wiley & Sons, Ltd.     

Modelling and predicting the spatial dispersion of skin cancer considering environmental and socio-economic factors using a digital earth approach

ABSTRACT

Almost all causative factors of diseases depend on location. The Digital Earth approach is suitable for studying diseases globally. Geospatial information systems integrated with statistical models can be used to model the relationship between a disease and its causative factors. Through modelling, the most important causative factors can be extracted and the epidemiology of the disease can be observed. In this paper, skin cancer (the most common type of cancer) has been modelled based on its causative factors, including climate factors, people's occupations, nutrition habits, socio-economic factors, and usage of chemical fertiliser. To fit the model, a data framework was first designed, and then data were gathered and processed. Finally, the disease was modelled using Generalised Linear Models (GLM), a statistical model based on the location of the factors. The results of this study identify the most important causative factors together with their relative priority. Furthermore, a model was used to predict the change in skin cancer occurrences caused by a change in one of its causative factors. This work illustrates the ability of the model to predict disease occurrence. Thus, by using this Digital Earth approach, skincancer can be studied in all the key countries around the world.     

A Novel Multiple-Kernel Support Vector Regression Algorithm for Estimation of Water Quality Parameters

Natural Resources Research - The quality of surface waters plays a key role in the sustainability of ecological systems. Measuring water quality parameters (WQPs) is of high...     

Seismic design factors for RC special moment resisting frames in Dubai, UAE

This study investigates the seismic design factors for three reinforced concrete (RC) framed buildings with 4, 16 and 32-stories in Dubai, UAE utilizing nonlinear analysis. The buildings are designed according to the response spectrum procedure defined in the 2009 International Building Code (IBC’09). Two ensembles of ground motion records with 10% and 2% probability of exceedance in 50 years (10/50 and 2/50, respectively) are used. The nonlinear dynamic responses to the earthquake records are computed using IDARC-2D. Key seismic design parameters are evaluated; namely, response modification factor (R), deflection amplification factor (Cd), system overstrength factor (Ωo), and response modification factor for ductility (Rd) in addition to inelastic interstory drift. The evaluated seismic design factors are found to significantly depend on the considered ground motion (10/50 versus 2/50). Consequently, resolution to the controversy of Dubai seismicity is urged. The seismic design factors for the 2/50 records show an increase over their counterparts for the 10/50 records in the range of 200%-400%, except for the Ωo factor, which shows a mere 30% increase. Based on the observed trends, period-dependent R and Cd factors are recommended if consistent collapse probability (or collapse prevention performance) in moment frames with varying heights is to be expected.