Effects of polynuclear aromatic hydrocarbons on lysosomal membranes in molluscs

Lysosomal sequestration of polynuclear aromatic hydrocarbons (PNAHs), a major class of environmental contaminant, is a well-established phenomenon;¹ considerably less is known about their pathological effects on lysosomes. Marine molluscs contain a number of lysosome-rich tissues and PNAHs are known to induce deleterious alterations in lysosomal structure and latency of lysosomal enzymes.² The latter are presumed to involve destabilisation of the lysosomal membrane, resulting in increased permeability and reduced enzyme latency. If lysosomal injury involves derangement of membrane-lipid structure due to the interaction of PNAHs then it would be expected that membrane damage would be closely linked to the structural characteristics of the intruding molecule. Our results show that the effects of the isomeric PNAHs phenanthrene and anthracene on digestive cell lysosomal stability were markedly different in the marine mussel (Mytilus edulis) over the same range of tissue concentrations. Lysosomal membrane stability was determined using a cytochemical test for enzyme latency.³     

A modified linear analysis of a wave-energy conversion buoy

The analysis of a pneumatic-type wave-energy conversion buoy is developed assuming independence of the buoy heaving motion and the motion of the water column within the center pipe. Results of the analysis are then compared with experimental data in a study of the relative air velocity within the turbine passage. The results compare very well. The effect of the variation of the center pipe length is found to be significant for periods about the surge chamber resonance but is negligible in the neighborhood of the heaving resonance period. Further, the theory is applied to a prototype buoy study of the U.S. Coast Guard, and a dimensionless design curve is developed from the results of the prototype analysis.     

Microbiological Studies Relevant to a Lobster Introduction into Fatty Basin,British Columbia

Studies in marine microbiology relevant to the cultivation of lobster in Fatty Basin were made. Biomass of bacteria and allied microorganisms in whole seawater column of the basin was very small (2×10⁴ gC), but a large biomass was found in the sediments (3×10⁵ gC). The decomposition of chitin occurred chiefly in the sediments. The rate of decomposition (500 g/day) was approximately half of the rate of production. However, the remaining production was considered not to be involved in the chitin cycle of the basin. This hypothesis was supported by the results of the analysis of the budget of organic matter in the area. Shell disease of lobster caused by chitinoclastic bacteria was detected, although it was not serious. A destructive yeast parasite of crustaceans,Metschnikowia, was collected only from a crab in the basin. This report is dedicated to Professor KenSugawara for his 70 years old anniversary.