Quantifying inorganic sources of geochemical energy in hydrothermal ecosystems, Yellowstone National Park, USA

Combining analytical data from hot spring samples with thermodynamic calculations permits a quantitative assessment of the availability and ranking of various potential sources of inorganic chemical energy that may support microbial life in hydrothermal ecosystems. Yellowstone hot springs of diverse geochemical composition, ranging in pH from <2 to >9 were chosen for this study, and dozens of samples were collected during three field seasons. Field measurements of dissolved oxygen, nitrate, nitrite, total ammonia, total sulfide, alkalinity, and ferrous iron were combined with laboratory analyses of sulfate and other major ions from water samples, and carbon dioxide, hydrogen, methane, and carbon monoxide in gas samples to evaluate activity products for ∼300 coupled oxidation-reduction reactions. Comparison of activity products and independently calculated equilibrium constants leads to values of the chemical affinity for each of the reactions, which quantifies how far each reaction is from equilibrium. Affinities, in turn, show systematic behavior that is independent of temperature but can be correlated with pH of the hot springs as a proxy for the full spectrum of geochemical variability. Many affinities are slightly to somewhat dependent on pH, while others are dramatically influenced by changes in chemical composition. All reactions involving dissolved oxygen as the electron acceptor are potential energy sources in all hot spring samples collected, but the ranking of dominant electron donors changes from ferrous iron, and sulfur at high pH to carbon monoxide, hydrogen, and magnetite as pH decreases. There is a general trend of decreasing energy yields depending on electron acceptors that follows the sequence: O₂(aq) > NO₃⁻ ≈ NO₂⁻ ≈ S > pyrite ≈ SO₄⁻² ≈ CO(g) ≈ CO₂(g) at high pH, and O₂(aq) ≈ magnetite > hematite ≈ goethite > NO₃⁻ ≈ NO₂⁻ ≈ S ≈ pyrite ≈ SO₄⁻² at low pH. Many reactions that are favorable sources of chemical energy at one set of geochemical conditions fail to provide energy at other conditions, and vice versa. This results in energy profiles supplied by geochemical processes that provide fundamentally different foundations for chemotrophic microbial communities as composition changes.     

Elemental,isotopic, and structural changes in Tagish Lake insoluble organic matter produced by parent body processes

Here, we present the results of a multitechnique study of the bulk properties of insoluble organic material (IOM) from the Tagish Lake meteorite, including four lithologies that have undergone different degrees of aqueous alteration. The IOM C contents of all four lithologies are very uniform and comprise about half the bulk C and N contents of the lithologies. However, the bulk IOM elemental and isotopic compositions vary significantly. In particular, there is a correlated decrease in bulk IOM H/C ratios and δD values with increasing degree of alteration—the IOM in the least altered lithology is intermediate between CM and CR IOM, while that in the more altered lithologies resembles the very aromatic IOM in mildly metamorphosed CV and CO chondrites, and heated CMs. Nuclear magnetic resonance (NMR) spectroscopy, C X‐ray absorption near‐edge (XANES), and Fourier transform infrared (FTIR) spectroscopy confirm and quantitate this transformation from CR‐like, relatively aliphatic IOM functional group chemistry to a highly aromatic one. The transformation is almost certainly thermally driven, and probably occurred under hydrothermal conditions. The lack of a paramagnetic shift in ¹³C NMR spectra and 1s‐σ* exciton in the C‐XANES spectra, both typically seen in metamorphosed chondrites, shows that the temperatures were lower and/or the timescales were shorter than experienced by even the least metamorphosed type 3 chondrites. Two endmember models were considered to quantitatively account for the changes in IOM functional group chemistry, but the one in which the transformations involved quantitative conversion of aliphatic material to aromatic material was the more successful. It seems likely that similar processes were involved in producing the diversity of IOM compositions and functional group chemistries among CR, CM, and CI chondrites. If correct, CRs experienced the lowest temperatures, while CM and CI chondrites experienced similar more elevated temperatures. This ordering is inconsistent with alteration temperatures based on mineralogy and O isotopes.     

Simulating the impact of the large-scale circulation on the 2-m temperature and precipitation climatology

The impact of the simulated large-scale atmospheric circulation on the regional climate is examined using the Weather Research and Forecasting (WRF) model as a regional climate model. The purpose is to understand the potential need for interior grid nudging for dynamical downscaling of global climate model (GCM) output for air quality applications under a changing climate. In this study we downscale the NCEP-Department of Energy Atmospheric Model Intercomparison Project (AMIP-II) Reanalysis using three continuous 20-year WRF simulations: one simulation without interior grid nudging and two using different interior grid nudging methods. The biases in 2-m temperature and precipitation for the simulation without interior grid nudging are unreasonably large with respect to the North American Regional Reanalysis (NARR) over the eastern half of the contiguous United States (CONUS) during the summer when air quality concerns are most relevant. This study examines how these differences arise from errors in predicting the large-scale atmospheric circulation. It is demonstrated that the Bermuda high, which strongly influences the regional climate for much of the eastern half of the CONUS during the summer, is poorly simulated without interior grid nudging. In particular, two summers when the Bermuda high was west (1993) and east (2003) of its climatological position are chosen to illustrate problems in the large-scale atmospheric circulation anomalies. For both summers, WRF without interior grid nudging fails to simulate the placement of the upper-level anticyclonic (1993) and cyclonic (2003) circulation anomalies. The displacement of the large-scale circulation impacts the lower atmosphere moisture transport and precipitable water, affecting the convective environment and precipitation. Using interior grid nudging improves the large-scale circulation aloft and moisture transport/precipitable water anomalies, thereby improving the simulated 2-m temperature and precipitation. The results demonstrate that constraining the RCM to the large-scale features in the driving fields improves the overall accuracy of the simulated regional climate, and suggest that in the absence of such a constraint, the RCM will likely misrepresent important large-scale shifts in the atmospheric circulation under a future climate.     

Resistance among wild invertebrate populations to recurrent estuarine acidification

Acid sulphate soils (ASS), which occur on floodplains worldwide, pose a significant threat to estuarine ecosystems. In laboratory and field experiments, naïve calcifying organisms that are exposed for even short periods (1–2 mo) to runoff from ASS suffer 80% mortality and slowed growth. Based on these observations we expected that sampling of wild oyster, gastropod and crab populations at sites close to and away from drains discharging ASS runoff would reveal more depauperate populations, of sparser and smaller-sized individuals at the more acidified sites. Sampling within three estuaries of New South Wales, Australia, confirmed that the oyster Saccostrea glomerata and gastropods (primarily Bembicium auratum) were less abundant at ASS-affected than reference sites. Nevertheless, crab abundances did not differ between the acidified and reference sites and impacts to bivalves and gastropods were far smaller than predicted. Although at ASS-affected sites gastropod populations were dominated by smaller individuals than at reference sites, oyster populations were skewed towards larger individuals. Even at ASS-affected sites, oyster and gastropod abundances were within the range encountered in estuaries that are not influenced by ASS runoff. Behaviour, long-term physiological acclimation or genetic selection may be responsible for differences in the responses of wild and naïve macroinvertebrates to acidification. Alternatively, wild populations may exhibit some recovery between the rainfall events that transport ASS runoff into estuaries, despite the persistently lower pH near outflow drains. Irrespective, this study suggests that at the population level, calcifying organisms display a certain degree of natural resistance to recurrent disturbance from ASS runoff.     

Carbon flows in the benthic food web at the deep-sea observatory HAUSGARTEN (Fram Strait)

The HAUSGARTEN observatory is located in the eastern Fram Strait (Arctic Ocean) and used as long-term monitoring site to follow changes in the Arctic benthic ecosystem. Linear inverse modelling was applied to decipher carbon flows among the compartments of the benthic food web at the central HAUSGARTEN station (2500 m) based on an empirical data set consisting of data on biomass, prokaryote production, total carbon deposition and community respiration. The model resolved 99 carbon flows among 4 abiotic and 10 biotic compartments, ranging from prokaryotes up to megafauna. Total carbon input was 3.78±0.31 mmol C m⁻² d⁻¹, which is a comparatively small fraction of total primary production in the area. The community respiration of 3.26±0.20 mmol C m⁻² d⁻¹ is dominated by prokaryotes (93%) and has lower contributions from surface-deposit feeding macro- (1.7%) and suspension feeding megafauna (1.9%), whereas contributions from nematode and other macro- and megabenthic compartments were limited to <1%. The high prokaryotic contribution to carbon processing suggests that functioning of the benthic food web at the central HAUSGARTEN station is comparable to abyssal plain sediments that are characterised by strong energy limitation. Faunal diet compositions suggest that labile detritus is important for deposit-feeding nematodes (24% of their diet) and surface-deposit feeding macrofauna (∼44%), but that semi-labile detritus is more important in the diets of deposit-feeding macro- and megafauna. Dependency indices on these food sources were also calculated as these integrate direct (i.e. direct grazing and predator–prey interactions) and indirect (i.e. longer loops in the food web) pathways in the food web. Projected sea-ice retreats for the Arctic Ocean typically anticipate a decrease in the labile detritus flux to the already food-limited benthic food web. The dependency indices indicate that faunal compartments depend similarly on labile and semi-labile detritus, which suggests that the benthic biota may be more sensitive to changes in labile detritus inputs than when assessed from diet composition alone. Species-specific responses to different types of labile detritus inputs, e.g. pelagic algae versus sympagic algae, however, are presently unknown and are needed to assess the vulnerability of individual components of the benthic food web.     

Dust in the solar system and in extra-solar planetary systems

Among the observed circumstellar dust envelopes a certain population, planetary debris disks, is ascribed to systems with optically thin dust disks and low gas content. These systems contain planetesimals and possibly planets and are believed to be systems that are most similar to our solar system in an early evolutionary stage. Planetary debris disks have been identified in large numbers by a brightness excess in the near-infrared, mid-infrared and/or submillimetre range of their stellar spectral energy distributions. In some cases, spatially resolved observations are possible and reveal complex spatial structures. Acting forces and physical processes are similar to those in the solar system dust cloud, but the observational approach is obviously quite different: overall spatial distributions for systems of different ages for the planetary debris disks, as opposed to detailed local information in the case of the solar system. Comparison with the processes of dust formation and evolution observed in the solar system therefore helps understand the planetary debris disks. In this paper, we review our present knowledge of observations, acting forces, and major physical interactions of the dust in the solar system and in similar extra-solar planetary systems.     

Climatic change in Central Asia during MIS 3/2: a case study using biological responses from Lake Baikal

A Marine Isotope Stage (MIS) 3/early MIS 2 section from a structural high along the east coast of the North Basin of Lake Baikal was analysed for diatoms, C/N ratios, and organic carbon isotope ratios. Diatoms were present throughout MIS 3 and early MIS 2, with high concentrations of the planktonic taxa Cyclotella sp. c.f. gracilis between 54 and 51.5 kyr BP indicating relatively warm, interstadial, conditions. Following a %TOC inferred climatic cooling between 43.2 and 39.1 kyr BP, evidence of a more muted δ¹³C_(organic) and %TOC inferred climatic warming from c. 39.1–34.7 kyr BP coincides with a period of very high diatom concentrations, indicating high aquatic productivity, at the Buguldeika Saddle in the South Basin of Lake Baikal. No evidence exists for a ‘Kuzmin’ catchment erosional event in the North Basin during MIS 3. This, however, may reflect the location of the coring site away from major riverine inputs. Abrupt climatic cooling at the culmination of both warm phases in the North Basin are associated, on the basis of the palaeomagnetic age-model and correlations to existing sites in Lake Baikal, with the initiation of Heinrich events 5 (c. 50 kyr BP) and 4 (c. 35 kyr BP), respectively, in the North Atlantic. The amount of organic material declines across the MIS 3/MIS 2 transition while constant C/N ratios suggest organic material to be predominantly derived from phytoplankton. An increase in δ¹³C_(organic) at the MIS 3/MIS 2 transition may therefore indicate changes in aquatic productivity, pCO₂ or the inorganic carbon pool.     

A high‐resolution multi‐proxy stalagmite record from Mechara,Southeastern Ethiopia: palaeohydrological implications for speleothem palaeoclimate reconstruction

An annually laminated stalagmite from Southeastern Ethiopia grew for 443±8 yr starting at 5023 yr BP (±160 yr) as determined by lamina‐tuned U‐Th dating. The speleothem shows three growth phases: (1) an initial phase where the stalagmite has a candlestick shape, deposited by relatively slow rate drip water; (2) a middle growth phase where laminae grow on the flanks, suggesting faster drip rates; and (3) a final growth phase where the speleothem becomes narrower owing to a decrease in drip water supply towards the end of deposition. Morphometry, annual growth rate, fluorescence index, and ²³⁴U/²³⁸U isotope ratio show marked differences between the growth phases, while δ¹³C and δ¹⁸O do not show significant variation, except within the third phase towards the top of the stalagmite, where they have higher values. The study indicates that the multiproxy approach is crucial as the different proxies respond to single climate forcing in different manners. Our results can be widely applied to speleothem studies distinguishing the relative importance of the various proxies in recording cave ‘external’ (climate, hydrology) and cave ‘internal’ (e.g. evaporation) processes. Copyright © 2006 John Wiley & Sons, Ltd.     

Preliminary studies concerning subsurface probes for the exploration of icy planetary bodies

It is well-known that the permanent terrestrial ice sheets (glaciers and polar caps) contain a lot of information about the recent geological history and in particular about climatic changes. Extrapolating this fact to other ice sheets in the solar system (e.g. the Mars polar regions, the icy moons of the outer planets, etc.), we may expect a similar wealth of information. To obtain this information it is possible to drill holes or melt the ice by a heated probe, which in this way is able to penetrate the surface and investigate the deeper layers in situ. In the latter case the driving agent is the heating power and the weight of the probe. In this paper we consider the application of such “melting probes” for exploring the structure of ice sheets in extraterrestrial environments. We describe several laboratory experiments with simple melting probes performed under cryo-vacuum conditions and compare the results with tests in a terrestrial environment. The experiments revealed that under space conditions the downward motion of a heated probe in an ice sheet is characterized by intermittent periods of sublimation and melting of the surrounding ice, sometimes interrupted by periods where a part of the probe's outer surface is frozen to the surrounding ice. This leads to a temporary blocking of the probe's downward motion. A similar situation can occur when the trailing tether is frozen in behind the probe. During the periods of ice sublimation the penetration process is significantly more power consuming, due to the large difference between the latent heat of sublimation and the latent heat of melting for water ice.     

Neurophysiological control of swimming behaviour,attachment and metamorphosis in black-footed abalone (Haliotis iris) larvae

Experiments were conducted to test the effect of a range of chemicals on larval responses in swimming behaviour, attachment and metamorphosis of the black-footed abalone (Haliotis iris). The effect of antibiotics on larval survival was first tested within negative (filtered seawater) and positive (GABA at 10^?5, 10^?4 and 10^?3 mol L^?1) control assays over 3 days. This experiment corroborated the effectiveness of using antibiotics to improve survival of larvae without obvious synergistic interactions with the GABA inducer or confounding effects of potential bacterial interactions. Chemical treatments (acetylcholine, potassium chloride, dopamine and glutamine) were then tested at various concentrations for their ability to modulate swimming behaviour and induce larval attachment and metamorphosis over 14 days. Generally, larval state shifted from swimming to attached, and from attached to metamorphosed, in the control and treatments over time. However, the peak percentage of attached and metamorphosed larvae varied in time among chemicals and concentrations. While overall percent metamorphosis was minimally enhanced after 14 days of exposure to some chemical treatments at certain concentrations, all treatments displayed significant capacities to down-regulate larval swimming and induce early attachment and metamorphosis. Mortality was recorded throughout the duration of the experiment, and was generally low (<20%) across controls and most treatments for exposures of less than 12 days. Interpretations of specific results from this study are used to elucidate neurophysiological control of larval activities for this abalone species. Comparisons with other marine invertebrates highlight the specificities of chemical cues and endogenous regulatory mechanisms across relatively closely related taxa.