Ex situ cultivation of Aplysinaaerophoba close to in situ conditions: ecological, biochemical and histological aspects

Sponges provide the largest number of biologically active natural products known from the marine environment and continue to be a very well studied phylum of marine fauna. The Mediterranean sponge Aplysina aerophoba accumulates brominated isoxazoline alkaloids such as Aplysinamisin-1, Aerophobin-2, Isofistularin-3 and the biotransformation product Aeroplysinin-1, which possesses, for example, antibiotic and cytotoxic properties. Until now, it is still being discussed which organisms – the sponge itself or associated microorganisms – are responsible for metabolite production. For cultivating Aplysina individuals under ex situ conditions, we surveyed relevant ecological factors in situ and controlled them in our aquarium system. We maintained A. aerophoba for more than 9 months and analysed changes of metabolite content and composition, microbial association as well as morphology in situ and ex situ under different light exposure. Although sponges showed slight reduction during maintenance, ex situ cultivation similar to in situ conditions provides a promising method to keep sponges and obtain their bioactive metabolites.     

Explicit stress–strain equations for modeling frictional materials

In this paper, a comprehensive study on simulating the shearing behavior of frictional materials is performed. A set of two explicit equations, describing the relationship among the shear stress ratio and the distortional strain and the volumetric strain, are formulated independently. The equations contain three stress parameters and three strain parameters and another parameter representing the nonuniformity of stress and strain during softening. All the parameters have clear physical significance and can be determined experimentally. It is demonstrated that the proposed equations have the capacity of simulating the complicated shearing behavior of many types of frictional materials including geomaterials. The proposed equations are used to simulate the stress–strain behavior for 27 frictional materials with 98 tests. These materials include soft and stiff clays in both reconstituted and structured states, silicon sands and calcareous sands, silts, compacted fill materials, volcanic soils, decomposed granite soils, cemented soils (both artificially and naturally cemented), partially saturated soils, ballast, rocks, reinforced soils, tire chips, sugar, wheat, and rapeseed. It has been demonstrated that the proposed explicit constitutive equations have the capacity to capture accurately the shearing behavior of frictional materials both qualitatively and quantitatively. A study on model parameters has been performed.     

南海西北部沉积物捕获器中颗石藻通量和属种组成变化记录

本文利用沉积物捕获器回收的连续时间序列沉降颗粒物样品,研究了南海西北部海南岸外陆坡外缘海域颗石藻的通量和属种组成变化及其环境意义。研究发现,东亚夏季风引起的海南岸外上升流以及冬季风导致的混合层深度增加都会刺激该区域颗石藻的生长,其中夏季上升流的影响尤为显著。从颗石粒属种组成来看,Florisphaera profunda是南海西北部中深层(1 000m)水体中主要的颗石粒优势种,此外Emiliania huxleyi,Gephyrocapsa oceanica和Gephyrocaps ericsonii等属种含量也颇高。然而在将颗石粒折算成颗石球数量后,Emiliania huxleyi则成为主要优势种。沉积物捕获器中F.profunda的相对百分含量与颗石粒总通量呈显著的负相关,表明F.profunda的相对含量与总的颗石藻生产力有着密切联系。本结果对于利用沉积物中的颗石藻记录,尤其是F.profunda相对含量变化,恢复和重建古海洋环境尤其是古海洋生产力的研究提供了直接的理论证据。     

Numerical and Experimental Analysis of A Ducted Propeller Designed by A Fully Automated Optimization Process Under Open Water Condition

A fully automated optimization process is provided for the design of ducted propellers under open water conditions, including 3D geometry modeling, meshing, optimization algorithm and CFD analysis techniques. The developed process allows the direct integration of a RANSE solver in the design stage. A practical ducted propeller design case study is carried out for validation. Numerical simulations and open water tests are fulfilled and proved that the optimum ducted propeller improves hydrodynamic performance as predicted.     

Anthropogenic impacts on nitrogen fixation rates between restored and natural Mediterranean salt marshes

To test the effects of site and successional stage on nitrogen fixation rates in salt marshes of the Venice Lagoon, Italy, acetylene reduction assays were performed with Salicornia veneta‐ and Spartina townsendii‐vegetated sediments from three restored (6–14 years) and two natural marshes. Average nitrogen fixation (acetylene reduction) rates ranged from 31 to 343 μmol C₂H₄·m^?2·h^?1 among all marshes, with the greatest average rates being from one natural marsh (Tezze Fonde). These high rates are up to six times greater than those reported from Southern California Spartina marshes of similar Mediterranean climate, but substantially lower than those found in moister climates of the Atlantic US coast. Nitrogen fixation rates did not consistently vary between natural and restored marshes within a site (Fossei Est, Tezze Fonde, Cenesa) but were negatively related to assayed plant biomass within the acetylene reduction samples collected among all marshes. Highest nitrogen fixation rates were found at Tezze Fonde, the location closest to the city of Venice, in both natural and restored marshes, suggesting possible site‐specific impacts of anthropogenic stress on marsh succession.     

Biogenic carbon flows through the planktonic food web of the Amundsen Gulf (Arctic Ocean): A synthesis of field measurements and inverse modeling analyses

Major pathways of biogenic carbon (C) flow are resolved for the planktonic food web of the flaw lead polynya system of the Amundsen Gulf (southeast Beaufort Sea, Arctic Ocean) in spring-summer 2008. This period was relevant to study the effect of climate change on Arctic marine ecosystems as it was characterized by unusually low ice cover and warm sea surface temperature. Our synthesis relied on a mass balance estimate of gross primary production (GPP) of 52.5 ± 12.5 g C m⁻² calculated using the drawdown of nitrate and dissolved inorganic C, and a seasonal f-ratio of 0.64. Based on chlorophyll a biomass, we estimated that GPP was dominated by phytoplankton (93.6%) over ice algae (6.4%) and by large cells (>5 μm, 67.6%) over small cells (<5 μm, 32.4%). Ancillary in situ data on bacterial production, zooplankton biomass and respiration, herbivory, bacterivory, vertical particle fluxes, pools of particulate and dissolved organic carbon (POC, DOC), net community production (NCP), as well as selected variables from the literature were used to evaluate the fate of size-fractionated GPP in the ecosystem. The structure and functioning of the planktonic food web was elucidated through inverse analysis using the mean GPP and the 95% confidence limits of every other field measurement as lower and upper constraints. The model computed a net primary production of 49.2 g C m⁻², which was directly channeled toward dominant calanoid copepods (i.e. Calanus hyperboreus 20%, Calanus glacialis 10%, and Metridia longa 10%), other mesozooplankton (12%), microzooplankton (14%), detrital POC (18%), and DOC (16%). Bacteria required 29.9 g C m⁻², a demand met entirely by the DOC derived from local biological activities. The ultimate C outflow comprised respiration fluxes (82% of the initial GPP), a small sedimentation (3%), and a modest residual C flow (15%) resulting from NCP, dilution and accumulation. The sinking C flux at the model limit depth (395 m) supplied 60% of the estimated benthic C demand (2.8 g C m⁻²), suggesting that the benthos relied partly on other C sources within the bottom boundary layer to fuel its activity. In summary, our results illustrate that the ongoing decline in Arctic sea ice promotes the growth of pelagic communities in the Amundsen Gulf, which benefited from a ∼80% increase in GPP in spring-summer 2008 when compared to 2004 – a year of average ice conditions and relatively low GPP. However, 53% of the secondary production was generated within the microbial food web, the net ecological efficiency of zooplankton populations was not particularly high (13.4%), and the quantity of biogenic C available for trophic export remained low (6.6 g C m⁻²). Hence it is unlikely that the increase in lower food web productivity, such as the one observed in our study, could support new harvestable fishery resources in the offshore Beaufort Sea domain.