The impact of natural and anthropogenic forcings on climate and hydrology since 1550

A climate simulation of an ocean/atmosphere general circulation model driven with natural forcings alone (constant “pre-industrial” land-cover and well-mixed greenhouse gases, changing orbital, solar and volcanic forcing) has been carried out from 1492 to 2000. Another simulation driven with natural and anthropogenic forcings (changes in greenhouse gases, ozone, the direct and first indirect effect of anthropogenic sulphate aerosol and land-cover) from 1750 to 2000 has also been carried out. These simulations suggest that since 1550, in the absence of anthropogenic forcings, climate would have warmed by about 0.1 K. Simulated response is not in equilibrium with the external forcings suggesting that both climate sensitivity and the rate at which the ocean takes up heat determine the magnitude of the response to forcings since 1550. In the simulation with natural forcings climate sensitivity is similar to other simulations of HadCM3 driven with CO₂ alone. Climate sensitivity increases when anthropogenic forcings are included. The natural forcing used in our experiment increases decadal–centennial time-scale and large spatial scale climate variability, relative to internal variability, as diagnosed from a control simulation. Mean conditions in the natural simulation are cooler than in our control simulation reflecting the reduction in forcing. However, over certain regions there is significant warming, relative to control, due to an increase in forest cover. Comparing the simulation driven by anthropogenic and natural forcings with the natural-only simulation suggests that anthropogenic forcings have had a significant impact on, particularly tropical, climate since the early nineteenth century. Thus the entire instrumental temperature record may be “contaminated” by anthropogenic influences. Both the hydrological cycle and cryosphere are also affected by anthropogenic forcings. Changes in tree-cover appear to be responsible for some of the local and hydrological changes as well as an increase in northern hemisphere spring snow cover.

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Structure of the Transkei Basin and Natal Valley, Southwest Indian Ocean, from seismic reflection and potential field data

Marine geophysical data from the southern Natal Valley and northern Transkei Basin, offshore southeast Africa, were used to study the structure of the crust and sedimentary cover in the area. The data includes seismic reflection, gravity and magnetics and provides information on the acoustic basement geometry (where available), features of the sedimentary cover and the basin's development. Previously mapped Mesozoic magnetic anomalies over a part of the basin are now recognized over wider areas of the basin. The ability to extend the correlation to the southeast within the Natal Valley further confirms an oceanic origin for this region and provides an opportunity to amplify the existing plate boundary reconstructions.The stratigraphic structure of the southern Natal Valley and the northern Transkei Basin reflects processes of the ocean crust formation and subsequent evolution. The highly variable relief of the acoustic basement may relate to the crust formation in the immediate vicinity of the continental transform margin. Renewed submarine seismicity and neotectonic activity in the area is probably related to the diffuse boundary between the Nubia and Somalia plates.2.5-D crustal models show that a 1.7–3.2-km-thick sediment sequence overlies a 6.3±1.2-km-thick normal oceanic crust in the deep southern Natal Valley and Transkei Basin. The oceanic crust in the study area is heterogeneous, made up of blocks of laterally varying remanent magnetization (0.5–3.5 A/m) and density (2850–2900 kg/m³). Strong modifications of accretionary processes near ridge/fracture zone intersections may be a reason of such heterogeneity.     

Interannual Variability Influences Brown Tide (<Emphasis Type="Italic">Aureococcus anophagefferens</Emphasis>) Blooms in CoastalEmbayments

Blooms of Aureococcus anophagefferens in Chincoteague Bay were observed during 5 of 6 years between 2002 and 2007. In order to understand factors controlling blooms, interannual differences in nitrogen and carbon uptake and concentrations of dissolved constituents were compared at two sites in Chincoteague Bay, MD and VA over the 6-year time period. Over that time, we observed that there was no single nitrogen compound that fueled blooms each year. Instead, A. anophagefferens took up a wide range of nitrogen compounds to meet its nutritional demands. Although photosynthetic carbon fixation was the dominant form of carbon acquisition during blooms, organic carbon uptake contributed up to 30 % of the total carbon uptake. In addition to interannual variability in nitrogen and carbon uptake, we observed that there was an increase in bloom intensity and duration over the 6-year study period during which dissolved organic carbon appeared to accumulate in the system.     

Stress distribution over the Mozambique Ridge

The Mozambique Ridge is an aseismic oceanic plateau in the southwestern Indian Ocean. During the separation of Antarctica and South Africa in the Early Cretaceous, the Mozambique Ridge was segmented by fracture zones which were assumed to become inactive during the Cenomanian, when Africa and Antarctica were finally separated. However, the existence of active normal faulting in the central part of the Mozambique Ridge was demonstrated by single and multichannel seismic surveys. Numerical modelling of the stress distribution caused by the crustal structure of the Mozambique Ridge and the adjacent oceanic basins suggests the possible existence of a zone with average horizontal tension up to 70 MPa along the central part of this passive ridge, which may cause the modern fault activity. These stresses also cause an additional dynamic anomaly which can explain small variations of the geoid anomaly over the ridge.     

Early to Middle Devonian granitic and volcanic rocks from the central Gulf of Maine

Cashes Ledge igneous suite in the central Gulf of Maine is represented by 10 granitic and two felsic tuff samples collected from bedrock outcrops using the submersible Alvin in 1971–1972 and archived at the Woods Hole Oceanographic Institute. Laser ablation ICP-MS analyses of zircon grains yielded crystallization ages of 414.9 ± 1.1 Ma and 399.7 ± 1.5 Ma for two alkali feldspar granite samples, 407.0 ± 1.9 Ma for a syenogranite sample, and 384.4 ± 2.3 Ma and 383.9 ± 1.6 Ma for two felsic tuff samples. The samples contain iron-rich mafic minerals, including aegirine-augite, grunerite/ferroedenite, and annite. Most of the samples are alkaline to slightly peralkaline, with high concentrations of SiO₂, Y, Zr, Nb, and REE, strong negative Eu anomalies, and positive epsilon Nd values (1.8 to 3.7). The suite resembles part of a belt of similar Silurian–Devonian rocks with ages between 426 and 370 Ma now recognized in the central part of Avalonia in southeastern New England. They formed in a long-lived, likely extensional regime linked to subduction and subsequent complex transcurrent motions among Ganderia, Avalonia, and Meguma, culminating in the closure of the Rheic Ocean.     

Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols

Benzo(a)pyrene (BaP) and polychlorinated biphenyls (PCBs) often co-exist in contaminated environments. Polychlorobiphenylols (OH-PCBs), formed by CYP-dependent monooxygenation of PCBs, are potent inhibitors of the glucuronidation of hydroxylated BaP metabolites. We hypothesized that OH-PCBs could drive the biotransformation of (−)BaP-7,8-dihydrodiol (BaP-7, 8-D) away from detoxication and towards formation of the reactive metabolite. A mixture of five OH-PCBs with 4–6 Cl atoms was infused into isolated, perfused, biliary intact livers (n=3 fish) removed from 3-methylcholanthrene-induced channel catfish. Controls (n=3) were infused with vehicle. Subsequently, [³H]-BaP-7, 8-D was infused into each liver and bile was collected for 1 h. The livers were taken for analysis of metabolites and DNA adducts. Induction status was confirmed by EROD assay. Bile was analyzed for metabolites. It was found that preinfusion of the mixture of OH-PCBs reduced the extent of glucuronidation of BaP-7, 8-D and increased the formation of DNA adducts 5-fold over controls. GSH conjugates, tetrols and triols were increased in the OH-PCB-infused fish, providing further support for our hypothesis that if the glucuronidation were inhibited, CYP-dependent activation would increase. These studies suggest a mechanism for synergy of toxicity of PAH and PCBs.     

Glucuronidation in the polar bear (Ursus maritimus)

Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 μg/ml, but dropped off very sharply at higher protein concentrations. The apparent K_m for 3-OH-BaP was 1.71 ± 0.04 μM, and V_max 1.26 ± 0.16 nmol/min/mg protein (mean ± SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4^′-OH-CB72) and a model hexachloro-OH-PCB (4^′-OH-CB159) were assayed with [¹⁴-C]-UDPGA and separation of the [¹⁴-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [¹⁴-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4^′-OH-CB72 (K_m 7.3 μM; V_max 1.55 nmol/min/mg) than 4^′-OH-CB159 (K_m 16.1 μM; V_max 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver.     

Catalytic properties of cytochrome P-450 in hepatopancreas of the spiny lobster, Panulirus argus

These studies were designed to determine the metabolic capability of the microsomal cytochrome(s) P-450 in spiny lobster hepatopancreas, and to determine how chemicals which selectively modify mammalian monooxygenase activity catalyzed by different cytochrome P-450 isozymes affect the spiny lobster cytochrome P-450. We used a washing procedure to concentrate the hepatopancreas microsomal cytochrome P-450 and remove the inhibitors of monooxygenase activity which are normally present in microsomes. The resulting reparation (MI fraction) was used to determine monooxygenase activity towards benzo[a]pyrene, benz-phetamine, 7-ethoxyresorufin in the presence of either cumene hydroperoxide or NADPH and vertebrate liver cytochrome P-450 reductase. Benzphetamine was the best substrate for the lobster cytochrome P-450, whereas 7-ethoxyresorufin was metabolized very slowly. Studies with chemical modifiers showed that the responses of the lobster cytochrome(s) P-450 were not similar to those of any of the well-characterized cytochrome P-450 isozymes purified from mammalian liver.