Induction of heat shock protein (hsp)60 in Isochrysis galbana exposed to sublethal preparations of dispersant and Prudhoe Bay crude oil

Adaptation to sublethal exposure to crude oil by phytoplankton is poorly understood. Use of chemical dispersants for oil spill remediation increases petroleum hydrocarbon concentrations in water, while exposing marine organisms to potentially toxic concentrations of dispersant. Heat shock proteins (hsps) have been found to serve as an adaptive and protective mechanism against environmental stresses. The objective of this project was to examine the induction of hsps in Isochrysis galbana, a golden-brown algae, following exposure to the water-accommodated fraction (WAF) of Prudhoe Bay crude oil (PBCO) and PBCO chemically dispersed with Corexit 9527® (dispersed oil: DO). Initial experiments using ³⁵S-labeled amino acids and 2-dimensional electrophoresis with subsequent western blotting identified and confirmed hsp60, a member of the chaperonin family of stress proteins, as being efficiently induced by heat shock in this species. One-dimensional SDS PAGE and western blotting, with hsp60 antibodies and chemiluminesence detection, were used to quantitate hsp60 following exposure to a range of environmental temperatures and concentrations of WAF and DO preparations. I. galbana cultured in 22 parts per thousand (‰) salinity showed a statistically significant increase (p<0.05) in hsp60 after exposure to 25, 30, and 35°C for 1 h compared to controls (20°C), while 34‰ cultures showed no significant increase in hsp60 with increasing temperature. Cultures (22 and 34‰) exposed to varying doses of WAF and DO exhibited statistically significant (p<0.05) increases in levels of hsp60 in all cases. A dose-related response was observed in all exposures (r²≥0.90) with the exception of 34‰ WAF. In independent exposures, a dose-related hsp60 response to naphthalene, a relatively abundant water-soluble aromatic hydrocarbon, was observed in both 22 and 34‰ cultures. Results of this study are consistent with previous studies in other species documenting increases in hsp60 levels with exposure to xenobiotics. Further studies are investigating the protective function of hsp60 against the toxic effects of exposure to WAF and DO preparations.     

The Effect of Macrofauna on Porewater Profiles and Nutrient Fluxes in the Intertidal Zone of the Humber Estuary

Macrofauna, nutrient fluxes, porewater chemistry and sediment characteristics were measured at six intertidal mudflat sites in the Humber Estuary, U.K., during the different seasons. Nereis diversicolor , Macoma balthica and Corophium volutator were found to be the dominant macrofauna. Salinity was the baseline control on macrofauna distribution but this was overprinted by periodic impoverishment due to sediment mobilization. High resolution gel probe porewater samplers provided direct evidence for the impact of burrows on porewater chemistry. The macrofauna modified nutrient fluxes during periods of mud flat stability. Nereis caused a decrease in silicate and phosphate effluxes but enhanced ammonia release and nitrate uptake. Macoma enhanced ammonia and nitrite release. The impact of Corophium was not possible to discern. The Humber is a large, highly dynamic macrotidal estuary in which sediment resuspension has a large impact on porewater profiles, nutrient fluxes and macrofaunal communities. Simple patterns and inter-relationships which are seen in small sheltered estuaries are not observed in the Humber.     

Evaluation of the Variability of the Shoreline of the Tsimlyansk Reservoir and Lake Ilmen according to Space Sounding Data

Izvestiya, Atmospheric and Oceanic Physics - This article presents the results of a study of the dynamics of the shorelines of Lake Ilmen and the Tsimlyansk Reservoir to indicate the location of...     

On Methods of Short-Term Earthquake Prediction Based on Monitoring the Hydrogeodeformation Field

Izvestiya, Atmospheric and Oceanic Physics - The permanent analysis of the hydrogeodeformation (HGD) field is one promising direction in the field of predicting strong earthquakes. As a result of...     

Anthropogenic Meso-Meteorological Feedbacks: A Review of a Recent Research

Izvestiya, Atmospheric and Oceanic Physics - The anthropogenic impact on the Earth’s climate system is currently one of the main factors determining climate change over all spatial scales,...     

Applied Aspects of Different Frequency Bands of Seismic and Water Acoustic Investigations on the Shelf

Izvestiya, Atmospheric and Oceanic Physics - This article focuses on the study of the information value of offshore technologies based on the generation of acoustic signals in water in a wide...     

Intense turbulent convection in a horizontal plane liquid layer

Direct numerical simulation of turbulent convection in a horizontal liquid layer heated from below is performed within the framework of the nonstationary Navier—Stokes equations with the use of the Bubnov—Galerkin method. The main attention is given to calculations for superhigh supercriticalities. Computational burden is reduced by the use of the splitting method at each step of integration. Previously, the smallness of the residual arising from substitution of simulated results into the initial system of equations is demonstrated and the residual’s dependence on the number of reference functions and supercriticality is considered. A good agreement of the results obtained with the use of different numerical implementations of the Bubnov—Galerkin procedure is shown, in particular, for the stochastic processes corresponding to a low supercriticality and appearing with the formation of strange attractors close to a Mobius strip. The calculations were carried out for a wide range of supercriticality (from 1 to 34000). It is shown that simulations and experiment are in good qualitative agreement.     

Hydrophysical state of the Large Aral Sea in the autumn of 2013: Thermal structure,currents, and internal waves

This paper is focused on the hydrophysical state of the western basin of the Large Aral Sea in its present conditions of ecological crisis. The investigation is based on the direct observations carried out during a field survey in the Aral Sea in October of 2013. The analysis of the distribution of the temperature values across the western basin is given. Time series of the water currents variability were recorded at four mooring stations. The pattern of the basin-scale circulation response to the wind forcing was described. Based on the in situ measurements of the time variability of the temperature fields, the principal characteristics of the internal waves were specified for the present-day Aral Sea for the first time.     

Analysis of EMD algorithm for identification and extraction of an acoustic signal in underwater channel against wind driven ambient noise

Sonar generated acoustic signals transmitted in underwater channel for distant communications are affected by numerous factors like ambient noise, making them nonlinear and non-stationary in nature. In recent years, the application of Empirical Mode Decomposition （EMD） technique to analyze nonlinear and non-stationary signals has gained much attention. It is an empirical approach to decompose a signal into a set of oscillatory modes known as intrinsic mode functions （IMFs）. In general, Hilbert transform is used in EMD for the identification of oscillatory signals. In this paper anew EMD algorithm is proposed using FFT to identify and extract the acoustic signals available in the underwater channel that are corrupted due to various ambient noises over a range of 100 Hz to 10 kHz in a shallow water region.Data for analysis are collected at a depth of 5 m and 10 m offshore Chennai at the Bay of Bengal. The algorithm is validated for different sets of known and unknown reference signals. It is observed that the proposed EMD algorithmidentifies and extracts the reference signals against various ambient noises. Significant SNR improvement is alsoachieved for underwater acoustic signals.