Mixed-function oxygenases as biological monitors around petroleum hydrocarbon development sites: Potential for induction by diesel and other drilling mud base oils containing reduced levels of polycyclic aromatic hydrocarbons

The use of oil-based drilling muds has been discouraged in hydrocarbon exploration and production in the marine environment but these muds are presently being used to a considerable degree in the United Kingdom and Norwegian sectors of the North Sea. Field studies in the North Sea have demonstrated only localized impacts around individual drilling sites,^1,2 even including those sites where ‘toxic’ diesel oils were employed as base fluids in drilling muds. Yet the question of disposal of cuttings contaminated with oil from drilling muds remains somewhat controversial. The induction of mixed-function oxygenases (MFO) has been validated on a number of occasions in the field as a sensitive index of low level hydrocarbon exposure (reviewed by Payne),³ including sites in the North Sea where diesel-based muds were used.⁴ The present study demonstrates that any potential for induction by hydrocarbon contaminated cuttings will probably be reduced by substitution of low-aromatic base oils for diesel in drilling mud formulations.     

Wave drift enhancement effects in multi column structures

A theoretical assessment is made of mean wave drift forces on groups of vertical circular cylinders, such as the columns of a floating offshore platform. A complete analytical solution is obtained for two cylinders extending from seabed to free surface, and a long wave approximation is found to provide reliable predictions of the drift force in line with the waves at low frequencies. For moderate separation between the two cylinders, this force is found to tend at low frequencies to a value four times the force on an isolated cylinder.A numerical method is employed to study two surface piercing cylinders truncated below the free surface, and an arrangement of four vertical cylinders characteristic of a floating offshore platform. The mean vertical drift force is found to be reasonably well approximated, over the frequency range of practical interest, by the force on an individual cylinder considered in isolation multiplied by the number of cylinders in the group. Interaction effects, however, have a profound influence on the total horizontal drift force. At low frequencies this force is found to tend to the force on an isolated cylinder multiplied by the squate of the number of cylinders in the group.     

An experimental investigation of plunging breaker boundary layers in the transformation zone

Experimental investigation is made on the boundary layers of the transformation zone (i.e. the region between the last symmetrical wave profile depth and the breaking point) of plunging breakers propagating on a smooth beach with 1/12 uniform slope. Using a laser anemometer, the particle velocities are measured at four verticals along the transformation zone for three different steepnesses of waves within the plunging breaker range. The boundary layer flow in the transformation zone is found mostly of turbulent character and vertical distribution of particle velocities does not seem to conform to the classical law of the wall distribution given for steady-flow boundary layers. The results show that free-stream particle velocities, in the boundary layer of the breaker under the crest phase, increase considerably as the wave progresses towards the breaking point. The boundary layer thickness, defined as the velocity-affected region, remains constant throughout the transformation zone but it decreases with increasing deep-water wave steepness for the particular beach slope tested.     

Design of a caisson plate under wave impact

From the laboratory experiments and field studies it has been shown that when a wave breaks directly on a vertical wall, impact pressures of high magnitude and short duration, are produced. Despite the recent advances made in collecting data on impact pressure histories and their spatial distributions, analyses on the structural behaviour of the walls loaded by the impact forces do not seem adequate. In the present study the theoretical analysis of the response characteristics of a caisson plate, having different aspect ratios, under the wave impact loading is investigated. Numerical results for the dynamic values of moments and transverse displacements are obtained by the method of finite elements. Some prerequisite experimental data for wave breaking and resulting impact pressures are provided. The static results for moments and deflections are also computed for comparing them with the dynamic values. The dynamic results are found significantly greater than the static values. The ratio between the dynamic and static values is called “Dynamic magnification factor” that varies with plate aspect ratio. Based on this factor a procedure is proposed which may have practical consequences in the design of caisson plates.     

The effect of currents on the generation of internal waves by a barotropic tide near an underwater ridge

In the frame of the linear theory for long waves, the paper studies long waves generated by a barotropic tide running at an arbitrary angle over a bottom ridge. The ocean is assumed to be two-layered. In the area of a ridge with a rectangular cross section, geostrophic flows are considered, coupled with the inclinations of the free surface and interface. Wave amplitudes are shown to depend on the angle of an onrunning tide and this allows us to reach a conclusion about the influence of a geostrophic current on the generation of internal waves. Translated by Vladimir A. Puchkin.     

The Amphipod Community as a Bioindicator in Algeciras Bay (Southern Iberian Peninsula) Based on a Spatio-Temporal Distribution

Abstract. The amphipod population associated with Bugula neritina (L.), a verycommon bryozoan species in Algeciras Bay (Strait of Gibraltar), was studied on a spatio-temporal scale, in order to define its role as a bioindicator. Samples were collected in December, March, June and September along a transect running from the external to the internal sampling stations.
Fifty-three species belonging to 22 families were identified; the most abundant species were Jassa marmorata H olmes , Ischyrocerus inexpectatus (R uffo ) and Phtisica marina S labber .
The spatial evolution of the amphipod community reflected the physico-chemical conditions of Algeciras Bay, yielding a clear gradient from the outer to the inner stations. The stations located at the mouth of the bay, characterised by high hydrodynamism and low sedimentation, had a higher diversity and species richness than the inner stations with low water movement and higher sedimentation. Eight species preferred for the outer stations, while another nine species were typical of the inner harbours.
The most notable aspect of the seasonal evolution was the increased dominance of the tube-builder and deposit-suspension feeder Jassa marmorota .
Structural and ordination analyses corroborate the outer-inner gradient in the bay and illustrate the importance of hydrodynamic forces and sedimentation in the amphipod community.     

The distribution of tritium and CFCs in the Weddell Sea during the mid-1980s

Transient tracer data (tritium, CFC11 and CFC12) from the southern, central and northwestern Weddell Sea collected during Polarstern cruises ANT III-3, ANT V-2/3/4 and during Andenes cruise NARE 85 are presented and discussed in the context of hydrographic observations. A kinematic, time-dependent, multi-box model is used to estimate mean residence times and formation rates of several water masses observed in the Weddell Sea.Ice Shelf Water is marked by higher tritium and lower CFC concentrations compared to surface waters. The tracer signature of Ice Shelf Water can only be explained by assuming that its source water mass, Western Shelf Water, has characteristics different from those of surface waters. Using the transient nature of tritium and the CFCs, the mean residence time of Western Shelf Water on the shelf is estimated to be approximately 5 years. Ice Shelf Water is renewed on a time scale of about 14 years from Western Shelf Water by interaction of this water mass with glacial ice underneath the Filchner-Ronne Ice shelf. The Ice Shelf Water signature can be traced across the sill of the Filchner Depression and down the continental slope of the southern Weddell Sea. On the continental slope, new Weddell Sea Bottom Water is formed by entrainment of Weddell Deep Water and Weddell Sea Deep Water into the Ice Shelf Water plume. In the northwestern Weddell Sea, new Weddell Sea Bottom Water is observed in two narrow, deep boundary currents flowing along the base of the continental slope. Classically defined Weddell Sea Bottom Water (θ ≤ −0.7°C) and Weddell Sea Deep Water (−0.7°C ≤ θ ≤ 0°C) are ventilated from the deeper of these boundary currents by lateral spreading and mixing. Model-based estimates yield a total formation rate of 3.5Sv for new Weddell Sea Bottom Water (θ = −1.0°C) and a formation rate of at least 11Sv for Antarctic Bottom Water (θ = −0.5°C).     

Effects of spectrum band width on the distribution of wave heights and periods

The statistical distribution of the crest-to-trough heights of narrowband nonlinear sea waves is derived in a semi-closed form. A quantitative comparison of the resulting density and exceedance probability distributions with those of the linear theory is given. It is shown that the nonlinearity of waves, even with steepnesses typical of extreme sea states, has an insignificant influence on the distribution of crest-to-trough heights.     

Sedimentology and morphodynamics of a macrotidal beach, Pendine Sands, SW Wales

The foreshore of Pendine Sands forms the seaward part of an extensive, sandy coastal barrier in a shallow Carmarthen Bay, SW Wales. The sedimentological features of the macrotidal foreshore reflect a tide-induced modification of nearshore wave characteristics. As the tide ebbs, the breaker height may decrease, the surf zone widens and becomes increasingly dissipative, and swash/backwash velocities diminish. A concomitant change from plunging to spilling breakers and increasingly symmetrical swash zone flows are associated with a decreasing beach gradient.

A zero net transport model demonstrates that the beach profile is self-stabilising in the short-term, and periodic levelling has shown that the beach is in long-term equilibrium with prevailing conditions, though this does not preclude a significant dynamic response to changing tides and waves.

The flow regimes of wave-generated currents decline as the tide ebbs, and normal beach processes do not usually affect the lower foreshore. Accordingly, there is an overall seaward-fining of the primary framework component of the sands. In more detail, this framework component displays a slight seaward-coarsening across an upper foreshore dominated by high water swash and surf; a rapid seaward-fining across the mid-foreshore in response to the ebb-attenuating swash zone flow velocities; and a slight seaward-fining across the lower foreshore under the action of nearshore shoaling waves. Bedforms vary from a swash/backwash emplaced flat bed across the upper foreshore to the small ripples of nearshore asymmetric oscillatory flows across the lower foreshore.

The surface sediment veneer is not representative of the subsurface sediments which form in response partly to fairweather conditions, partly to storms. The upper foreshore is characterised by swash/backwash emplaced plane bedding in fine sands frequently disrupted by bubble cavities. The mid-foreshore is composed of coarser-grained shelly traction clogs arranged as landward- and seaward-dipping large-scale cross bedding and/or plane bedding; these are probably storm breaker/surf deposits. The lower foreshore, though partially and sometimes totally bioturbated, shows landward-dipping small-scale cross bedding in very fine sands sorted by nearshore shoaling waves.

Tide- and storm-induced modification of the nearshore flow regimes therefore produces a distinctive shore-normal array of sedimentary facies. Each facies is characterised by diagnostic textural and structural signatures. A prograding sequence of such macrotidal deposits would be similar to, but more extensive than, a comparable microtidal sequence.     

A fish hepatocyte model for the investigation of the effects of environmental contaminants

An isolated fish hepatocyte culture system was developed as a models ystem to investigate the mechanisms of action of environmental contaminants. Hepatocytes were isolated from striped bass (Morone saxatilis) by an adaptation of the two stage perfusion technique of Seglen.¹ The system was used to evaluate metal binding protein (MBP) induction in response to cadmium, a primary inducer of metallothionein (MT) in rat hepatocytes. Striped bass hepatocytes appeared to be refractory to the induction of MBP by cadmium, since there was no significant increase in the synthesis of MBP for any of the doses at any of the time points investigated. However, when a similar experiment was performed using rat hepatocytes there was induction of MBP that was related to both dose and time. These comparative experiments indicate that although there are similarities between the hepatocytes of the two species in regard to ³⁵S incorporation into low molecular weight metal-binding proteins, there appear to be significant quantitative differences as well in regard to MBP kinetics. This in vitro model system could potentially be utilized to investigate the toxicological properties of other environmental contaminants.