黄鳍鲷LDH同工酶的组织特异性研究

以黄鳍鲷为材料进行两方面研究:（1）用聚丙烯酰胺凝胶电泳方法，对其肌肉，心脏，肝脏，血清，玻璃体，晶状体，脑等组织器官进行LDH同工酶分析比较，结果表明，不同组织中LDH的同工酶谱带不相同，存在着组织特异性：（2）用聚丙烯酰胺等电聚焦电泳法对其肌肉，血清的LDH同工酶进行分离 ，结果得到12－15条电泳区带，等电点范围在pH为3．65－9．58之间。     

Quaternary geology of the Amazonian Lowland

The Quaternary history of the Amazon lowlands is characterized by deposition of sediments of Andean provenance and by the influences of changing sea levels. Areas well above the present water tables were not reached by Pleistocene high-water stages. These areas have been intensively weathered since the Tertiary, forming hard lateritic weathering horizons. These weathering horizons are best explained by the relatively constant, humid tropical climate throughout the Quaternary. In the western Amazonian Lowland, flood plains corresponding to the different Pleistocene sea-level heights were formed. During low sea level, erosion in the drainage areas increased and the water levels of the central Amazon River system were lowered. Valleys drowned and lakes formed in the lower reaches of rivers and creeks during high sea-level stages. These lakes (ria lakes) remained in the valleys with rivers having a low sediment load. Seismic profiling (3.5 kHz) in some of these lakes clearly showed deposits of the three last periods of Quaternary high sea-level stages.     

Dissolved aluminium and the silicon cycle in the Arctic Ocean

Concentrations of dissolved (0.2 µm filtered) aluminium (Al) have been determined for the first time in the Eurasian part of the Arctic Ocean over the entire water column during expedition ARK XXII/2 aboard R.V. Polarstern (2007). An unprecedented number of 666 samples was analysed for 44 stations along 5 ocean transects. Dissolved Al in surface layer water (SLW) was very low, close to 1 nM, with lowest SLW concentrations towards the Canadian part of the Arctic Ocean and higher values adjacent to and in the shelf seas. The low SLW concentrations indicate no or little influence from aeolian dust input. Dissolved Al showed a nutrient-type increase with depth up to 28 nM, but large differences existed between the different deep Arctic basins. The differences in concentrations of Al between water masses and basins could largely be related to the different origins of the water masses. In the SLW and intermediate water layers, Atlantic and Pacific inflows were of importance. Deep shelf convection appeared to influence the Al distribution in the deep Eurasian Basin. The Al distribution of the deep Makarov Basin provides evidence for Eurasian Basin water inflow into the deep Makarov Basin. A strong correlation between Al and Silicon (Si) was observed in all basins. This correlation and the nutrient-like profile indicate a strong biological influence on the cycling and distribution of Al. The biological influence can be direct by the incorporation of Al in biogenic silica, indirect by preferential scavenging of Al onto biogenic siliceous particles, or by a combination of both processes. From the slope of the overall Al–Si relationship in the intermediate water layer (AIDW; ~ 200–2000 m depth), an Al/Si ratio of 2.2 atoms Al per 1000 atoms Si was derived. This ratio is consistent with the range of previously reported Al/Si uptake ratio in biogenic opal frustules of diatoms. In the deepest waters (>2000 m depth) a steeper slope of the Al–Si relationship of 7.4 to 13 atoms Al per 1000 atoms Si likely results from entrainment of cold shelf water into the deep basins, carrying the signal of dissolution of terrigenous particles with a much higher Al:Si ratio of crustal abundance. Only a small enrichment with such crustal Al and Si component may readily account for the higher Al:Si slope in the deepest waters.     

Developing a building policy for the erosion zone: Solutions to some key (Dutch) questions

In those coastal communities where the most seaward strip of mainland consists of dunes, these dunes often serve as a flexible sea defence. In addition, this strip offers large potential for housing and commercial enterprises. Unfortunately, due to severe storm surges part of this strip (the erosion zone) is subject to erosion, and as a result of which any buildings or infrastructure located here, are destroyed. Therefore, as we will illustrate in this paper, a building policy for this zone should reflect a compromise between two opposite interests: exploitation of the existing potential and, prevention of an unacceptable high risk due to erosion. Accordingly, the authors have developed a framework for such a building policy on the basis of which the desirability of various different types of investments and the location within the erosion zone of such investments can be determined. The examples that are used to illustrate this framework in this paper are limited to experiences in The Netherlands as relevant data and experiences are available and relatively easy accessible here. Nevertheless, the approach as is described is generic and applicable worldwide suggesting that the discovered unused potential for exploitation is not just limited to The Netherlands.     

The effect of a topographic high on the morphological stability of a two-inlet bay system

The cross-sectional stability of two tidal inlets connecting the same back-barrier lagoon to the ocean is investigated. The condition for equilibrium is the cross-sectional area tidal prism relationship. In an earlier study [Van de Kreeke, J., 1990. Can multiple inlets be stable? Estuarine, Coastal and Shelf Science 30: 261–273.], using the same equilibrium condition, it was concluded that where two inlets connect the same basin to the ocean ultimately one inlet will close. One of the major assumptions in that study was that the water level in the basin fluctuated uniformly. In hindsight this assumption might be too restrictive. For example, in the Wadden Sea the back barrier lagoon consists of a series of basins, rather than one single basin, separated by topographic highs. These topographic highs limit but do not exclude the exchange of water between the sub-basins. For this reason in the present study, a topographic high in the form of a weir was added, separating the back-barrier lagoon in two sub-basins. The water level in the sub-basins, rather than in the back-barrier as a whole, is assumed to fluctuate uniformly. For this schematization the hydrodynamic equations are solved using a finite difference method. The results, together with the equilibrium condition, yield the equilibrium flow curve for each of the inlets. The intersections of the two equilibrium flow curves represent combinations of cross-sectional areas for which both inlets are in equilibrium. The stability of the equilibriums was investigated using a non-linear stability analysis resulting in a flow diagram. Calculations were carried out for different inlet and weir characteristics. Sinussoidal tides were the same for both inlets. The results show that for relatively large wetted cross-sectional areas over the topographic high, approaching the situation of a single basin, there are no combinations of inlet cross-sectional areas for which both inlets are in a stable equilibrium. This supports the conclusion in the earlier study. For relatively small wetted cross-sectional areas over the topographic high there is one set of stable equilibriums. In that case the two-inlet bay system approaches that of two single-inlet bay systems.     

Large-scale dune erosion tests to study the influence of wave periods

Large-scale physical model tests were performed to quantify the effects of the wave period on dune erosion. Attention was focussed on 2D cross-shore effects in a situation with sandy dunes and extreme water levels and wave conditions. Besides profile measurements, detailed measurements in time and space of water pressure, flow velocities and sediment concentrations were performed in the near near-shore area. It was concluded that a longer wave period leads to a larger dune erosion volume and to a larger landward retreat of the dune face. Tests with double-peaked wave spectra showed that the influence of the spectral shape on dune erosion was best represented by the T_m − 1,0 spectral mean wave period, better than the peak wave period, T_p. The effect of the wave period on dune erosion was implemented in a dune erosion prediction method that estimates erosion volumes during normative storm conditions for the Dutch coast. More details of the measurements and additional analyses of physical processes are described in an accompanying paper by Van Thiel de Vries et al. [Van Thiel de Vries, J.S.M., van Gent, M.R.A., Reniers, A.J.H.M. and Walstra, D.J.R., submitted for publication. Analysis of dune erosion processes in large scale flume experiments, In this volume of Coastal Engineering.].     

Onshore sandbar migration at Tairua Beach (New Zealand): Numerical simulations and field measurements

We observed the onshore migration (3.5 m/day) of a nearshore sandbar at Tairua Beach, New Zealand during 4 days of low-energy wave conditions. The morphological observations, together with concurrent measurements of waves and suspended sediment concentrations, were used to test a coupled, wave-averaged, cross-shore model. Because of the coarse bed material and the relatively low-energy conditions, the contribution of the suspended transport to the total transport was predicted and observed to be negligible. The model predicted the bar to move onshore because of the feedback between near-bed wave skewness, bedload, and the sandbar under weakly to non-breaking conditions at high tide. The predicted bathymetric evolution contrasts, however, with the observations that the bar migrated onshore predominantly at low tide. Also, the model flattened the bar, while in the observations the sandbar retained its steep landward-facing flank. A comparison between available observations and numerical simulations suggests that onshore propagating surf zone bores in very shallow water (< 0.25 m) may have been responsible for most of the observed bar behaviour. These processes are missing from the applied model and, given that the observed conditions can be considered typical of very shallow sandbars, highlight a priority for further field study and model development. The possibility that the excess water transported by the bores across the bar was channelled alongshore to near-by rip-channels further implies that traditional cross-shore measures to judge the applicability of a cross-shore morphodynamic model may be misleading.     

Sediment bioavailable organic matter,deposition rates and mixing intensity in the Setúbal–Lisbon canyon and adjacent slope (Western Iberian Margin)

The role of the Setúbal–Lisbon canyon in accumulation and transport of labile organic matter from the coastal sea and ocean surface water towards the deep sea was assessed by investigating the distribution of organic matter of different quality in sedimentary aggregates and surface sediments of the canyon and adjacent slopes. Total hydrolysable amino acids (THAA) and organic carbon (Corg) were measured from aggregates, and contents of Corg, chlorophyll a (chl a), phaeopigments (phaeo), chloroplastic pigment equivalents (CPE) from sediments. As indices of organic matter (OM) quality THAA:Corg, degradation index (DI), chl a:phaeo, chl a:Corg and C:N ratio were determined. Sediment profiles of chl a and the isotope 210 of lead (²¹⁰Pb) were used as tracers in a transport model to estimate deposition rates and background levels of the tracers, and sediment mixing rates (Db). Whereas bulk Corg contents of canyon and slope sediments were practically similar at all depths, higher contents of THAA, chl a and CPE, as well as higher THAA:Corg, DI and chl a:Corg, in aggregates and sediments from the upper reaches of the canyon indicate that labile organic matter accumulates in the upper canyon. This is confirmed by higher chl a and ²¹⁰Pb deposition and Db calculated from the model. Hence, the Setúbal–Lisbon canyon, specially the upper region, acts as a natural trap of organic matter that is transported to the region via lateral transport and vertical settling from primary productivity. Organic matter might be further transported in downward canyon direction via rebound processes. The chl a and ²¹⁰Pb profiles reveal active sediment mixing by physical processes and/or animal reworking.     

Microbial photosynthesis in coral reef sediments (Heron Reef, Australia)

We investigated microphytobenthic photosynthesis at four stations in the coral reef sediments at Heron Reef, Australia. The microphytobenthos was dominated by diatoms, dinoflagellates and cyanobacteria, as indicated by biomarker pigment analysis. Conspicuous algae firmly attached to the sand grains (ca. 100 μm in diameter, surrounded by a hard transparent wall) were rich in peridinin, a marker pigment for dinoflagellates, but also showed a high diversity based on cyanobacterial 16S rDNA gene sequence analysis. Specimens of these algae that were buried below the photic zone exhibited an unexpected stimulation of respiration by light, resulting in an increase of local oxygen concentrations upon darkening. Net photosynthesis of the sediments varied between 1.9 and 8.5 mmol O₂ m⁻² h⁻¹ and was strongly correlated with Chl a content, which lay between 31 and 84 mg m⁻². An estimate based on our spatially limited dataset indicates that the microphytobenthic production for the entire reef is in the order of magnitude of the production estimated for corals. Photosynthesis stimulated calcification at all investigated sites (0.2–1.0 mmol Ca²⁺ m⁻² h⁻¹). The sediments of at least three stations were net calcifying. Sedimentary N₂-fixation rates (measured by acetylene reduction assays at two sites) ranged between 0.9 to 3.9 mmol N₂ m⁻² h⁻¹ and were highest in the light, indicating the importance of heterocystous cyanobacteria. In coral fingers no N₂-fixation was measurable, which stresses the importance of the sediment compartment for reef nitrogen cycling.