Induction of heat shock protein (hsp)60 in Isochrysis galbana exposed to sublethal preparations of dispersant and Prudhoe Bay crude oil

Adaptation to sublethal exposure to crude oil by phytoplankton is poorly understood. Use of chemical dispersants for oil spill remediation increases petroleum hydrocarbon concentrations in water, while exposing marine organisms to potentially toxic concentrations of dispersant. Heat shock proteins (hsps) have been found to serve as an adaptive and protective mechanism against environmental stresses. The objective of this project was to examine the induction of hsps in Isochrysis galbana, a golden-brown algae, following exposure to the water-accommodated fraction (WAF) of Prudhoe Bay crude oil (PBCO) and PBCO chemically dispersed with Corexit 9527® (dispersed oil: DO). Initial experiments using ³⁵S-labeled amino acids and 2-dimensional electrophoresis with subsequent western blotting identified and confirmed hsp60, a member of the chaperonin family of stress proteins, as being efficiently induced by heat shock in this species. One-dimensional SDS PAGE and western blotting, with hsp60 antibodies and chemiluminesence detection, were used to quantitate hsp60 following exposure to a range of environmental temperatures and concentrations of WAF and DO preparations. I. galbana cultured in 22 parts per thousand (‰) salinity showed a statistically significant increase (p<0.05) in hsp60 after exposure to 25, 30, and 35°C for 1 h compared to controls (20°C), while 34‰ cultures showed no significant increase in hsp60 with increasing temperature. Cultures (22 and 34‰) exposed to varying doses of WAF and DO exhibited statistically significant (p<0.05) increases in levels of hsp60 in all cases. A dose-related response was observed in all exposures (r²≥0.90) with the exception of 34‰ WAF. In independent exposures, a dose-related hsp60 response to naphthalene, a relatively abundant water-soluble aromatic hydrocarbon, was observed in both 22 and 34‰ cultures. Results of this study are consistent with previous studies in other species documenting increases in hsp60 levels with exposure to xenobiotics. Further studies are investigating the protective function of hsp60 against the toxic effects of exposure to WAF and DO preparations.     

Structure and Distribution of Autumn Zooplankton in the Southeastern Baltic Sea in 2015

Oceanology - Specific features of the structure and distribution of autumn zooplankton in the southeastern Baltic Sea (SEB) in October 2015 have been found taking into account the hydrological and...     

Rock moisture content in the field and the laboratory and its relationship to mechanical weathering studies

Rock moisture content is a major control of mechanical weathering, particularly freeze-thaw, and yet almost no data exist from field situations. This study presents moisture content values for rocks, taken from a variety of positions and conditions, in the maritime Antarctic. Additional information regarding the amount of water the rock could take up, as observed from laboratory experiments, is also presented. The results show that the approaches used in simulation experiments, particularly that of soaking a rock for 24 hours, may produce exaggerated results. It was found that the saturation coefficient (S-value) was a good indicator of frost susceptibility (based on water content) but that the derivation of that value may underestimate the potential of some rocks. The distribution of moisture within rocks is seen as an important, but unkown, factor. The results of these field moisture contents suggest that for simulations of freeze-thaw or hydration to be meaningful then they should have rock water contents based on field observations.     

Neo-deterministic seismic hazard assessment in North Africa

North Africa is one of the most earthquake-prone areas of the Mediterranean. Many devastating earthquakes, some of them tsunami-triggering, inflicted heavy loss of life and considerable economic damage to the region. In order to mitigate the destructive impact of the earthquakes, the regional seismic hazard in North Africa is assessed using the neo-deterministic, multi-scenario methodology (NDSHA) based on the computation of synthetic seismograms, using the modal summation technique, at a regular grid of 0.2?×?0.2°. This is the first study aimed at producing NDSHA maps of North Africa including five countries: Morocco, Algeria, Tunisia, Libya, and Egypt. The key input data for the NDSHA algorithm are earthquake sources, seismotectonic zonation, and structural models. In the preparation of the input data, it has been really important to go beyond the national borders and to adopt a coherent strategy all over the area. Thanks to the collaborative efforts of the teams involved, it has been possible to properly merge the earthquake catalogues available for each country to define with homogeneous criteria the seismogenic zones, the characteristic focal mechanism associated with each of them, and the structural models used to model wave propagation from the sources to the sites. As a result, reliable seismic hazard maps are produced in terms of maximum displacement (D _max), maximum velocity (V _max), and design ground acceleration.     

Electricity of the convective atmospheric boundary layer: Field observations and numerical simulation

The electric state of the middle-latitude convective atmospheric boundary layer (ABL) is investigated during a point in time when it is not disturbed by clouds, precipitation, mist, or industrial aerosols. A numerical model is developed that estimates the electroaerodynamic state of the convective ABL. The model is parameterized using results of field observations and laboratory experiments. According to the model, vertical profiles of atmospheric electric field strength, space charge density, electric conductivity, and atmospheric electric current density are calculated in the horizontally homogeneous approximation with a high space-time resolution.     

Analysis of EMD algorithm for identification and extraction of an acoustic signal in underwater channel against wind driven ambient noise

Sonar generated acoustic signals transmitted in underwater channel for distant communications are affected by numerous factors like ambient noise, making them nonlinear and non-stationary in nature. In recent years, the application of Empirical Mode Decomposition （EMD） technique to analyze nonlinear and non-stationary signals has gained much attention. It is an empirical approach to decompose a signal into a set of oscillatory modes known as intrinsic mode functions （IMFs）. In general, Hilbert transform is used in EMD for the identification of oscillatory signals. In this paper anew EMD algorithm is proposed using FFT to identify and extract the acoustic signals available in the underwater channel that are corrupted due to various ambient noises over a range of 100 Hz to 10 kHz in a shallow water region.Data for analysis are collected at a depth of 5 m and 10 m offshore Chennai at the Bay of Bengal. The algorithm is validated for different sets of known and unknown reference signals. It is observed that the proposed EMD algorithmidentifies and extracts the reference signals against various ambient noises. Significant SNR improvement is alsoachieved for underwater acoustic signals.     

Substrate control on formation and maturation of glauconites in the Middle Eocene Harudi Formation, western Kutch, India

Formation and maturation of glauconites within the early Middle Eocene Harudi Formation, built up in a lagoon to shelf transition in western Kutch, India is addressed taking recourse to a combined sedimentological, micropalaeontological and mineralogical investigation. The glauconite is authigenic and its occurrence coincides with maximum flooding. The glauconite is more matured within the fecal pellets and is less matured within the intra-particle pores of bioclasts namely, foraminifera, ostracoda, gastropoda and bryozoa. SEM-EDS, XRD and geochemical studies clearly document that the infilling within the intra-particle pores of bioclasts belong to nascent to slightly evolved glauconite (<5 wt% K₂O), while the pellet belongs to slightly evolved to evolved glauconite (5-7 wt% K₂O). Based on chemical characteristics and SEM investigations the glauconitization process can be best explained by the ‘verdissement theory’. The glauconite possibly formed as initial authigenic glauconitic smectite precipitates; while the pellet matured subsequently by addition of K, the process was hindered in case of infillings. The maturation process also involved addition of Si and release of Al, with or without release of Mg. The glauconite maturation was facilitated in case of fecal pellets because of higher porosity, inter-granular nature of pores and availability of necessary elements in semi-confined micro-environments. In contrast, the maturation was aborted at an early stage in case of infillings because of the minute, closed nature of the pores and prevalent alkaline conditions not allowing dissolution of calcitic tests. A low negative cerium anomaly suggests glauconite formation in sub-oxic micro-environments, created by decay of organic matters within the fecal pellets and bioclasts. A clear case of substratum control on glauconitization becomes apparent.     

Fishers' perceptions as indicators of the performance of Marine Protected Areas (MPAs)

How users perceive the performance of Marine Protected Areas (MPAs) is fundamental for the social acceptance of these zones. Moreover, their perceptions may be relevant for monitoring the effects of MPAs on extractive activities. This study analyzes artisanal fishers' perceptions of the performance of a north-western Mediterranean coastal MPA, which encompasses two no-take zones (NTZs). Three viewpoints have been considered: the effect on the personal activity of fishers, the effect on the local fishery and the effect on the ecosystem. In order to test the hypothesis that biomass export (spillover) - which had previously been evidenced from the two NTZs - may influence fishers' perceptions of NTZ effects, fishers' perceptions were compared with both declared and observed fishing activity over an one-year period.The results show that negative perceptions of NTZs are either nil or are negligible. Most fishers are aware of the beneficial effects of NTZs on ecosystems and fisheries. However, they remain to be convinced of the beneficial effects of the NTZs on their own activity. For instance, the proximity of a NTZ appears never to be involved in the choice of a fishing spot. This partial lack of correspondence between scientific expectation and fishers' perceptions is discussed in the light of fishing habits in the zone adjacent to NTZs, and takes into account fishing grounds, targeted species and seniority (defined as the number of years the fisher has been fishing within the MPA). All three factors appear to influence fishers' perceptions. For example, having a positive perception about a NTZ and spending more time fishing in the adjacent zone are habits that can be associated with fishers with less seniority. Fishers' perceptions obviously indicate the social acceptance of the MPA and are an essential monitoring tool for MPA managers. However, perceptions cannot be seen as a substitute for scientific monitoring, as both approaches are clearly complementary.     

Assessment of Dynamic Characteristics for Compaction Piles with Geophysical Logging in Soft Ground

In this paper, a case study was performed on a sand compaction pile (SCP) and a gravel compaction pile (GCP) to estimate the dynamic characteristics and the improvement effect of soft ground. The dynamic elastic modulus, shear modulus, bulk modulus, and Poisson's ratio were estimated and the dynamic characteristics were analyzed using the compression and shear wave velocity of the improved ground based on the results of suspension P- and S-wave (PS) logging. The results revealed that the dynamic properties were increased in the order of unimproved subsoil and improved subsoil using SCP and GCP. The increase in the effects of dynamic properties with each replacement ratio of SCP was not large, whereas a good increase in the effects was observed in the case of the improved subsoil with GCP. Consequently, it was presented that the resistance characteristics against the seismic loading of GCP are excellent. As a result of analyzing the density distribution of the improved subsoil through density field logging, the overall density distribution gradually exhibits increasing trends in the order of unimproved subsoil and improved subsoil with SCP and GCP. Thus, the improvement effect of GCP was relatively high in comparison with the same replacement ratio of SCP.