Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

Simulating a heavy metal spill under estuarine conditions: Effects on the clam Scrobicularia plana

We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry.     

Dynamic analysis of a vertical plate exposed to breaking wave impact

From the experimental studies in recent years, it has become known that when a wave breaks directly on a vertical faced coastal structure, high magnitude impact pressures are produced. The theoretical and experimental studies show that the dynamic response of such structures under wave impact loading is closely dependent on the magnitude and duration of the load history. The dynamic analysis and design of a coastal structure can be succeeded provided the design load history for the wave impact is available. Since these types of data are very scarce, it is much more convenient to follow a method which is based on static analysis for the dynamic design procedure. Therefore, to facilitate the dynamic design of a vertical plate that is exposed to breaking wave impact, a multiplication factor called “dynamic magnification factor” is herein presented which is defined as the ratio of the maximum value of the dynamic response to that found by static analysis. The computational results of the present study show that the dynamic magnification factor is a useful ratio to transfer the results of static analysis to the dynamic design of a coastal plate for the maximum impact pressure conditions of p_max/γH₀≤18.     

A new form of generalized Boussinesq equations for varying water depth

A new set of equations of motion for wave propagation in water with varying depth is derived in this study. The equations expressed by the velocity potentials and the wave surface elevations include first-order non-linearity of waves and have the same dispersion characteristic to the extended Boussinesq equations. Compared to the extended Boussinesq equations, the equations have only two unknown scalars and do not contain spatial derivatives with an order higher than 2. The wave equations are solved by a finite element method. Fourth-order predictor–corrector method is applied in the time integration and a damping layer is applied at the open boundary for absorbing the outgoing waves. The model is applied to several examples of wave propagation in variable water depth. The computational results are compared with experimental data and other numerical results available in literature. The comparison demonstrates that the new form of the equations is capable of calculating wave transformation from relative deep water to shallow water.     

Calibration of JASON-1 Altimeter over Lake Erie

This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr.     

Response of the barrier island coastal region of southwestern Nigeria to climate and non-climate forcing

Despite threats emanating from the influence of climate and non-climate forcing on the barrier island coastal region of southwestern Nigeria, the extent of the coastal erosion is poorly understood. We report evidence of coastal erosion and sediment accumulation in the region over a 34-year period (1973–2017), using Landsat imagery at intervals of approximately six years. Landsat image corrections and various water-extraction algorithms were used to systematically delineate coastal erosion and accumulation in the area. The region was subdivided into western and eastern subregions separated by Lagos Harbour. In the west, erosion took place during the periods 1973–1979, 1979–1984, 1990–1999 and 2005–2011, whereas in the east, erosion occurred during 1973–1979, 1990–1999 and 1999–2005. Coastal sediment accumulation occurred in the east during 1979–1984, 1984–1990, 2005–2011 and 2011–2017, whereas gains in the west occurred during 1984–1990, 1999–2005 and 2011–2017. The study revealed substantial net erosion of 1 228.1 ha in the region as a whole, over the full period. Sediment accumulation accompanying the coastal erosion appears to be linked to longshore drift. Erosion between 1973 and 2011 was probably attributable to climate change (storms and tidal conditions), longshore drift, the inflow and outflow of water at Lagos Harbour, coastal morphology and, possibly, human impacts. However, the coastal changes between 2011 and 2017 were more obviously associated with human activities, such as development of the Eko Atlantic construction project. Coastal surveillance, together with the use of environmentally sensitive protective measures, could possibly help to reduce coastal erosion in the region. Careful coastal management practices, including artificial nourishing and the installation of resilient structures (e.g. seawalls), should be undertaken to protect human settlements that are already at risk from sea-level rise.     

Retention and fate of experimentally added mercury in a Massachusetts salt marsh treated with sewage sludge

A simple, rapid, precise and reproducible procedure is described for sample preparation, extraction and measurement of total mercury in peaty sediments, plants and animal tissues. Various amounts of a heavy metal containing dried sewage sludge were applied to experimental plots in a New England salt marsh. This treatment resulted in elevation of mercury levels in the surface 5 cm of the soil. Discrepancies in the bulk density of marsh sediments corresponded with interparticle spaces and detritus/mineral ratios in the peat matrix. Mass balance calculations showed that wetlands covered with tall Spartina alterniflora lost mercury considerable faster than higher marsh locations which retained virtually all mercury added. The shortest halflife of mercury calculated was four years. Grain size analyses of peat and sewage sludge suggested that mercury in the higher intertidal range remained associated with sludge components. Biochemical and physical processes affecting the dissipation of mercury from creek side sediments are discussed.     

Observed foundation behaviour of concrete gravity platforms installed in the North Sea 1973–1978

Available measurements of foundation behaviour of the 13 concrete gravity platforms installed in the North Sea in the years 1973–1978 are presented. The measurements cover both the installation phase and the early part of the operation period. For the installation phase, measurements of dowel and skirt penetration resistance, base contact stresses, tilt, piping and erosion, are presented. For the operation period, the presentation includes measurements of settlements, pore water pressures, base contact stresses, permanent lateral displacements, cyclic displacements, dynamic behaviour and erosion. The measurements are used to back calculate soil properties and to evaluate the adequacy of the design procedures which have been used. The observations give valuable experience with respect to the foundation performance of offshore gravity platforms.     

Submerged cylinder wave energy device: theory and experiment

Linearized water wave theory is used to show that a submerged long circular cylinder suitably constrained by springs and dampers to make small harmonic oscillations, can be extremely efficient in absorbing the energy in an incident regular wave whose crests are parallel to the axis of the cylinder. Experimental results are described which confirm the theory for small amplitude waves and which suggest that the device can still be fairly efficient in waves of moderate amplitude.     

The crustal structure of Concepcion Bank

From reflection and refraction data a crustal model of the Concepcion Bank NE of Lanzarote Island has been calculated. It is interpreted as a tectonic unit of volcanic origin. Its evolution history of mainly Tertiary age does not depend on sea floor spreading. The Bank is built of intruded basaltic layers over material with a reduced mantle p-velocity.