Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.     

麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

莱州湾温带风暴潮预报研究

本文依据莱州湾羊角沟、夏营两站建国以来的风暴增水资料，对莱州湾建国后发生的风暴潮进行了统计分析，并探讨了温带风暴潮产生的物理机制，此外还对莱州湾温带风暴增水以及诱发增水的天气形势进行了分析分类。在此基础上建立了莱州湾温带风暴潮统计预报方法，并在作业预报中对模型进行了检验，取得较为理想的效果。     

花尾胡椒鲷养殖群体遗传多样性的等位酶研究

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。     

A fuzzy distinguishability on species composition similarities of pelagic fishes in different years in the southern Taiwan Strait

I\rnooUcrIoxThe drpdric fish species cor-npositlon is an imPOrtant element 1n the study of fish ecologyand plays an important guiding role ln fishery preduction and roouLrces rnanagement. Lin(l988) and Zhang (1994 and l995) have studied the simi1drities of mpulation COtwsition ofame s1ngle species of different yea-rs including hairtail Trichuras haumee and yellDw cndertwinena cmi. But no rePOrt is available on the discridrination of the sidrildrities amongmuLlt1ple sPecies composition of…     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

Seasonality and interannual variability of freshwater inflow to a large oligohaline estuary in the Northern Gulf of Mexico

Quantity, timing, duration, and fluctuation of freshwater inflow are important factors affecting the development and health of aquatic and adjacent wetland ecosystems in coastal estuaries. This study assessed six decades of freshwater inflow from the Amite River, Tickfaw River, and Tangipahoa River watersheds to Lake Pontchartrain, a large oligohaline estuary in the Northern Gulf of Mexico, whose flood waters caused recent damage to the city of New Orleans in the aftermath of Hurricane Katrina. By utilizing the long-term (1940–2002) river discharge and climatic data from the three major tributary watersheds, monthly and annual freshwater inflows have been quantified and their spatial and temporal variations have been analyzed. On average, the three rivers discharged (±standard error) 0.27 ± 0.04 km³ freshwater monthly and 3.29 ± 0.15 km³ freshwater annually into the lake estuarine system, with the highest inflow from the Amite River (0.16 ± 0.03 m³ mon⁻¹, and 1.91 ± 0.09 km³ yr⁻¹) and the lowest inflow from the Tickfaw River (0.03 ± 0.00 km³ mon⁻¹, and 0.34 ± 0.02 km³ yr⁻¹). A distinct seasonality was evident with over 69% of the total annual inflow occurring during December and May (wet months) and with a low flow period from August to November (dry months). The monthly inflow during the wet months was positively correlated with the monthly precipitation (r² = 0.64), while the monthly inflow during the dry months was subject to evapotranspiration. Furthermore, the study found a 20-year low flow period from 1954–1973 (2.76 ± 0.24 km³ yr⁻¹) and a 24-year high flow period from 1975–1998 (3.84 ± 0.24 km³ yr⁻¹), coinciding with both the climate variation and population growth in the watersheds.     

冲绳海槽北部西坡的古三角洲沉积

自20世纪70年代我国开展东海海洋地质调査以来,在海底经常能发现河流、河口沉积物以及与水道相似的负地形、称为古河道、古三角洲或古河口等(李全兴,1990；金翔龙,1992；石斯器等,1989；秦蕴珊等、1987)第四纪气候频繁波动，造成陆架上尤其是外陆架上陆相地层与海相地层频繁交替,因此有不同时期的古三角洲和古河道保留,沉溺的古河道在我国陆架海底的不少地区均有发现。由于海底沉积作用的不均衡性,埋藏较浅的沉溺河谷从地形特征上依稀可辨,埋藏较深的在海底表面则渺无踪迹,只有借助于高分辦率的地球物理测量,才能发现其踪迹。 晚更新世末期，长江、黄河是否流经东海陆架区进入冲绳海槽,虽有不少作者论述,但终因缺少系统的实测资料而未取得共识。长江、黄河在历史上每年向大海输入巨量泥沙,加快了海淤积和推进速度,但古长江三角洲也应该是体积大、构造特征明显的大型堆积体,然而至今尚未有这方面的报道。 作者对冲绳海槽北部进行大面积高分辨率浅地层剖面分析中,发现在陆架外缘坡折线附近,近海底表面处存在大面积斜层理三角洲沉积结构,这为晚更新世末期以来自我国的大河(黄河/长江)从冲绳海槽入海提供了新的证据。     

Surficial Sedimentary Diatoms in Okinawa Trough and Its Response to Kuroshio Current

This paper describes the relative contents of dominant diatom species and the distributive characteristics of tropical pelagic species and diatom assemblages, and also discusses the relations between the relative content of tropical pelagic species and Kuroshio Current and between the distribution of diatom assemblages and material sources. The results show that the limit of＞20% relative content of tropical pelagic species is basically consistent with the demarcation of Kuroshio Current, and that the distributions of dominant diatom species and diatom assemblages reserve the records of their closer relations to material sources.     

辽河盆地大民屯凹陷流体压力特征

大民屯凹陷是辽河断陷内4个下第三系凹陷之一。在综合利用钻井、试井及地震等资料的基础上，系统研究并论述了大民屯凹陷流体压力特征。基于57口井的声波测井资料，凹陷内泥岩压力特征可区分为正常压力、异常压力或强超压等类型；根据152口井391个点的压力测试数据，凹陷内产油层段的压力梯度多接近于1；利用公式法模拟计算了47条地震剖面的流体压力、剩余压力及压力系数的分布特征，凹陷内剖面压力系统自上而下一般由正常压力、弱超压和强超压3部分组成。此外，还根据流体压力演化的基本原理及钻井、岩性与试井等实际资料，模拟恢复了大民屯凹陷的压力演化史，其可划分为超压原始积累、超压部分释放及超压再积聚3个阶段。总体上，大民屯凹陷的超压强度低于渤海湾盆地其他地区的超压强度。