Properties of proteolytic toxin of Vibrio anguilolarum from diseased flounder

Extracellular products (ECP) produced byVibrio anguillarum strain M3 originally isolated from diseased flounder (Paralichthys olivaceus) were prepared. ECP of M3 showed gelatinase, casinase, amylase and haemolytic activity on agarose plates. High protease activity against azocasin was detected. Bacterium M2 showed highest growth and protease activity at 25°C. The protease present in ECP showed maximal activity at pH 8 and 55°C; was completely inactivated by application of 80°C heat for 30 min; was completely inhibited by EDTA and HgCl₂, and was partially inhibited by PMSF, SDS, MnCl₂ and iodoacetic acid; but not inhibited by CaCl₂ and MgCl₂. The ECP was toxic to flounder fish at LD₅₀ values of 3.1 μg protein/g body weight. The addition of HgCl₂ and application of heat at 50°C decreased the lethal toxicity of ECP. When heated at 100°C, ECP lethality to flounder was completely inhibited. After intramuscular injection of ECP into flounder, it showed evident histopathological changes including necrosis of muscle, extensive deposition of haemosiderin in the spleen, dilated blood vessels congested with numerious lymphocytes in the liver. These results showed that ECP protease was a lethal factor produced by the bacteriumV. anguillarum M3. Contribution No. 4213 from the Institute of Oceanology, Chinese Academy of Sciences. Funded by projects under the Major State Basic Research Development Program, Grant G1999012003.     

Magnetic properties and neutron diffraction study of two manganese sulfosalts: monoclinic MnSb<Subscript>2</Subscript>S<Subscript>4</Subscript> and benavidesite (MnPb<Subscript>4</Subscript>Sb<Subscript>6</Subscript>S<Subscript>14</Subscript>)

Mn²⁺Sb₂S₄, a monoclinic dimorph of clerite, and benavidesite (Mn²⁺Pb₄Sb₆S₁₄) show well-individualized single chains of manganese atoms in octahedral coordination. Their magnetic structures are presented and compared with those of iron derivatives, berthierite (Fe²⁺Sb₂S₄) and jamesonite (Fe²⁺Pb₄Sb₆S₁₄). Within chains, interactions are antiferromagnetic. Like berthierite, MnSb₂S₄ shows a spiral magnetic structure with an incommensurate 1D propagation vector [0, 0.369, 0], unchanged with temperature. In berthierite, the interactions between identical chains are antiferromagnetic, whereas in MnSb₂S₄ interactions between chains are ferromagnetic along c-axis. Below 6 K, jamesonite and benavidesite have commensurate magnetic structures with the same propagation vector [0.5, 0, 0]: jamesonite is a canted ferromagnet and iron magnetic moments are mainly oriented along the a-axis, whereas for benavidesite, no angle of canting is detected, and manganese magnetic moments are oriented along b-axis. Below 30 K, for both compounds, one-dimensional magnetic ordering or correlations are visible in the neutron diagrams and persist down to 1.4 K.     

Underestimation of roughness in rough rock joints

Numerous studies have been made to improve Barton's shear strength model for the quantification of rock joints. However, in these previous studies, the roughness and shear strength of the rock joint have been underestimated especially for relatively high undulated profiles (joint roughness coefficient (JRC) >14). The main factors of roughness underestimation in rough rock joints are investigated for the proper quantification of rock joint roughness. The aliasing effect and the roughness characteristics are analyzed by using artificial joint profiles and natural rock joint profiles. A 3D camera scanner is adopted to verify the main source of underestimation when using conventional measurement methods. Shear strength tests are carried out by using two types of shear apparatus to study the roughness mobilization characteristics, which may also affect the roughness underestimation. The results of joint roughness assessment, such as aliasing and undulation of waviness, show that the roughness can be underestimated in relatively rough joint profiles (JRC>14). At least two components of roughness parameters are needed to properly represent the joint roughness, for example, the amplitude and the inclination angle of joint asperity. Roughness mobilization is affected by both the normal stress and the asperity scale. Copyright © 2008 John Wiley & Sons, Ltd.     

Petrogenesis of the earliest Early Cretaceous mafic rocks from the Cona area of the eastern Tethyan Himalaya in south Tibet: Interaction between the incubating Kerguelen plume and the eastern Greater India lithosphere?

The relationship between the breakup of eastern Gondwanaland and the Kerguelen plume activity is a subject of debate. The Cona mafic rocks are widely exposed in the Cona area of the eastern Himalaya of south Tibet, and are studied in order to evaluate this relationship. Cona mafic rocks consist predominantly of massive basaltic flows and diabase sills or dikes, and are divided into three groups. Group 1 is composed of basaltic flows and diabase sills or dikes and is characterized by higher TiO₂ and P₂O₅ content and OIB-like trace element patterns with a relatively large range of Nd(T) values (+ 1.84 to + 4.67). A Group 1 diabase sill has been dated at 144.7 ± 2.4 Ma. Group 2 consists of gabbroic sills or crosscutting gabbroic intrusions characterized by lower TiO₂ and P₂O₅ content and “depleted” N-MORB-like trace element patterns with relatively higher, homogeneous Nd(T) values (+ 5.68 to + 6.37). A Group 2 gabbroic diabase dike has been dated at 131.1 ± 6.1 Ma. Group 3 basaltic lavas are interbedded with the Late Jurassic–Early Cretaceous pelitic sediments; they have compositions transitional between Groups 1 and 2 and flat to slightly enriched trace element patterns. Sr–Nd isotopic data and REE modeling indicate that variable degrees of partial melting of distinct mantle source compositions (enriched garnet–clinopyroxene peridotite for Group 1 and spinel-lherzolite for Group 2, respectively) could account for the chemical diversity of the Cona mafic rocks. Geochemical similarities between the Cona mafic rocks and the basalts probably created by the Kerguelen plume based on spatial–temporal constraints seem to indicate that an incubating Kerguelen plume model is more plausible than a model of normal rifting (nonplume) for the generation of the Cona mafic rocks. Group 1 is interpreted as being related to the incubating Kerguelen plume–lithosphere interaction; Group 2 is likely related to an interaction between anhydrous lithosphere and rising depleted asthenosphere enriched by a “droplet” originating from the Kerguelen plume, while Group 3 may be attributed to thermal erosion resulting in the partial melting of lithosphere during the long-term incubation of a magma chamber/pond at a shallow crustal level. The Cona mafic rocks are probably related to a progressively lithospheric thinning beneath eastern Gondwanaland from 150–145 Ma to 130 Ma. Our new observations seem to indicate that the Kerguelen plume may have started its incubation as early as the latest Jurassic or earliest Cretaceous period and that the incubating Kerguelen plume may play an active role in the breakup of Greater India, eastern India, and northwestern Australia.     

Holocene environmental changes around Xiaohe Cemetery and its effects on human occupation,Xinjiang, China

The Xiaohe Cemetery archaeological site (Cal. 4–3.5 ka BP) is one of the most important Bronze Age sites in Xinjiang, China. Although the surrounding environment is an extremely arid desert now, abundant archaeological remains indicate that human occupation was common during certain periods in the Holocene. Field investigations and laboratory analyses of a sediment profile near the Xiaohe Cemetery indicate that while the regional environment was arid desert throughout the Holocene there were three episodes of lake formation near the site in the periods 4.8–3.5 ka BP, 2.6–2.1 ka BP and 1.2–0.9 ka BP. Geomorphic and hydrological investigations reveal that a lake or lakes formed in a low-lying area when water was derived initially from the Kongque River and then shunted into the Xiaohe River basin. Low amounts of active chemical elements in lacustrine sediment between 4.8–3.5 ka BP indicate abundant and continuous water volume in the lake; the content of active chemical elements increased between 2.6–2.1 ka BP but was still at a relatively low level, suggesting a declining amount of water and diminished inflow. Between 1.2–0.9 ka BP there was a very high content of active elements, suggesting decreased water volume and indicating that the lake was stagnate. In contrast, the general climate condition shows that there had a warm-humid stage at 8–6 ka BP, a cool-humid stage at 6–2.9 ka BP and a warm-dry stage at 2.9–0.9 ka BP in this region. The hydrological evolutions around Xiaohe Cemetery did not have one-to-one correspondence with climate changes. Regional comparison indicates that broad-scale climatic conditions played an important role through its influences on the water volume of the Tarim River and Kongque River. But, the formation of the lakes and their level were controlled by geomorphic conditions that influenced how much water volume could be shunted to Xiaohe River from Kongque River. Human occupation of the Xiaohe Cemetery and nearby regions during the Bronze Age and Han-Jin period (202 BC–420 AD) corresponded to the two earlier lake periods, while no human activities existed in the third lake period because of the decreased water volume.     

海洋底质专题图中底质要素表示方法研究

针对当前海洋底质专题图制作标准不统一,表示方法不完善的情况,在分析已有底质专题要素表示方法的基础上,研究了海洋底质专题图的需求原则及表示原则,结合多种专题要素表示方法,制定了一种以纯色普染为基本方法的海洋底质专题图表示体系。通过实验对比可知,相较于其他表示方法,合理的纯色普染表达体系在表示海洋底质分布方面具有更好的效果和速率,为进一步推进海洋底质专题图的标准化生产提供了一定的借鉴。