Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Phylogenetic diversity of culturable bacteria in surface seawater from the Drake Passage,Antarctica

The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America. Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012. The 16S rRNA sequences were analyzed from 187 isolated bacterial strains. Three phyla, 29 genera and 56 species were identified. The three phyla were Actinobacteria, Firmicutes and Proteobacteria; the Proteobacteria included α-Proteobacteria, β-Proteobacteria and γ-Proteobacteria. γ-Proteobacteria, Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species. Gram-positive bacteria (Actinobacteria and Firmicutes) accounted for 57.8% of all types identified. There were nine dominant genera, including Curtobacterium, Staphylococcus, and Halomonas, and 14 dominant species including Curtobacterium flaccumfaciens, Curtobacterium pusillum, and Staphylococcus sciuri. Of the strains identified, 87.2% were catalase positive or weakly positive.     

Experimental study of motion characteristics of rock slopes with weak intercalation under seismic excitation

In order to investigate the effect of a weak intercalation on slope stability, a large-scale shaking table model test was conducted to study the dynamic response of rock slope models with weak intercalation. The dynamic response of the prototype slopes were studied in laboratory with the consideration of law of similitude. The initiation failure was observed in the rock slope model with a counter-tilt thin-weak intercalation firstly, not in the slope model with a horizontal thin-weak intercalation. Furthermore, it was interesting that the fracture site is shifted from crest top to the slope surface near the weak intercalation, which is different with the location of failure position in a normal layered slope. We also discussed the effect of the dip angle and the thickness of weak intercalation on the failure mechanism and instability mode of the layered rock slope. From the experimental result, it was noted that the stability of the slope with a counter-tilt weak intercalation could be worse than that of the other slopes under seismic excitation. The findings showed the difference of failure in slopes with a horizontal and counter weak intercalation, and implicated the further evaluation of failure of layered slopes caused by seismic loads.     

Development and installation of bedload monitoring systems with submerged load cells

Bedload governs riverbed channel variations and morphology, it is necessary to determine bedload discharge through an arbitrary cross section in a mountain river. A new system with submerged load cells has been developed to directly measure bedload discharge. The system consists of: (1) an iron box which is 1 m long, 0.5 m wide and 0.1 m in depth, (2) two submerged load cells 0.7 m apart, (3) a pressure sensor and, (4) an electromagnetic velocity meter. This system has been designed to exclude the effect of the hydraulic pressure of water on direct measurements of bedload particle weight. Initial tests in a laboratory were conducted to examine the accuracy of measurements with the system under aerial conditions. The system has been installed in the supercritical flume in Ashi-arai-dani River of the Hodaka Sedimentation Observatory of the Disaster Prevention Research Institute (DPRI) of Kyoto University to obtain bedload discharge under natural conditions. Flume tests were conducted in this channel by artificial supply of uniform sediment particles of several grain sizes. The average velocity of the sediment particles near the bed was estimated using cross-correlation functions for weight waves obtained by the two load cells. Bedload discharge calculations were based on time integration of the product of sediment velocity and sediment weight obtained by the two load cells. This study clarifies the reasons why bedload measurements are difficult, and provides some solutions using the monitoring systems with submerged load cells through the field measurements. Additionally, the applicability of bedload measurement with the submerged load cells is explained based on experimental artificial sediment supply data.     

Field observations of debris-flow initiation processes on sediment deposits in a previous deep-seated landslide site

Although information regarding the initiation processes of debris flows is important for the development of mitigation measures,field data regarding these processes are scarce.We conducted field observations of debris-flow initiation processes in the upper Ichinosawa catchment of the Ohya landslide,central Japan.On 19 June 2012,our videocamera monitoring systems recorded the moment of debris-flow initiation on channel deposits(nine surges) and talus slopes(eight surges).The initiation mechanisms of these surges were classified into three types by analyzing the video images: erosion by the surface flow,movement of deposits as a mass,and upward development of the fluid area.The first type was associated with the progress of surface flow from the upper stream on unsaturated channel deposits.The second type was likely caused by an increase in the pore water pressure associated with the rising in the groundwater level in channel deposits;a continuous water supply from the upper stream by the surface flow might have induced this saturation.The third type was associated with changes in the downstream topography caused by erosion.The flow velocity of most surges was less than 3 m s~(-1) and they usually stopped within 100 m from the initiation point.Surges with abundant pore fluid had a higher flow velocity(about 3- 5 m s~(-1)) and could travel for alonger duration.Our observations indicate that the surface flow plays an important role in the initiation of debris flows on channel deposits and talus slopes.     

Dynamic process simulation with a Savage-Hutter type model for the intrusion of landslide into river

Natural damming of rivers by mass movements is a very common and potentially dangerous phenomena which has been documented all over the world. In this paper, a two-layer model of Savage-Hutter type is presented to simulate the dynamic procedure for the intrusion of landslide into rivers. The two-layer shallow water system is derived by depth averaging the incompressible Navier-Stokes equations with the hydrostatic assumption. A high order accuracy scheme based on the finite volume method is proposed to solve the presented model equations. Several numerical tests are performed to verify the realiability and feasibility of the proposed model. The numerical results indicate that the proposed method can be competent for simulating the dynamic process of landslide intrusion into the river. The interaction effect between both layers has a significant impact on the landslide movement, water fluctuation and wave propagation.     

Spatial assessment of forest cover and land-use changes in the Hindu-Kush mountain ranges of northern Pakistan

Anthropogenic activities and natural processes are continuously altering the mountainous environment through deforestation, forest degradation and other land-use changes. It is highly important to assess, monitor and forecast forest cover and other land-use changes for the protection and conservation of mountainous environment. The present study deals with the assessment of forest cover and other land-use changes in the mountain ranges of Dir Kohistan in northern Pakistan, using high resolution multi-temporal SPOT-5 satellite images. The SPOT-5 satellite images of years 2004, 2007, 2010 and 2013 were acquired and classified into land-cover units. In addition, forest cover and land-use change detection map was developed using the classified maps of 2004 and 2013. The classified maps were verified through random field samples and Google Earth imagery (Quick birds and SPOT-5). The results showed that during the period 2004 to 2013 the area of forest land decreased by 6.4%, however, area of range land and agriculture land have increased by 22.1% and 2.9%, respectively. Similarly, barren land increased by 1.1%, whereas, area of snow cover/glacier is significantly decreased by 21.3%. The findings from the study will be useful for forestry and landscape planning and can be utilized by the local, provincial and national forest departments; and REDD+ policy makers in Pakistan.     

Length of stay in urban areas of circular migrants from the mountainous areas in China

Currently, there is limited literature dealing with the length of stay of circular migrants in urban areas (LSCMU), although many studies have discussed the phenomenon of migration and the determinants of migration. This study attempts to fill that gap using survey data from the mountainous areas of Chongqing, Southwest China. From a comparative perspective, this study divided workers into two groups (a young group aged between 16 and 35 years and an old group aged between 36 and 65 years). The average LSCMU values for the young and old groups were 225 days and 174 days, respectively. Two multi-regression models were used to estimate the determinants of LSCMU in the two groups. The results showed that LSCMU was closely related to individual factors in both groups, including gender, age and job training. Family and community factors, including household size, arable land per capita and the distance from market, had much weaker effects on the dependent variable, especially in the older group. It was noticeable that job training had significant positive effects on LSCMU in both groups; these findings have special implications for the urbanization process in China.     

Altitudinal trends in δ<Superscript>13</Superscript>C value,stomatal density and nitrogen content of <Emphasis Type="Italic">Pinus tabuliformis</Emphasis> needles on the southern slope of the middle Qinling Mountains,China

In this study, a coniferous tree species (Pinus tabuliformis Carr.) was investigated at a moderate-altitude mountainous terrain on the southern slope of the middle Qinling Mountains (QLM) to detect the trends in carbon isotope ratio (δ¹³C), leaf nitrogen content (LNC) and stomatal density (SD) with altitude variation in north-subtropical humid mountain climate zone of China. The results showed that LNC and SD both significantly increased linearly along the altitudinal gradient ranging from 1000 to 2200 m, whereas leaf δ¹³C exhibited a significantly negative correlation with the altitude. Such a correlation pattern differs obviously from that obtained in offshore low-altitude humid environment or inland high-altitude semi-arid environment, suggesting that the pattern of increasing δ¹³C with the altitude cannot be generalized. The negative correlation between δ¹³C and altitude might be attributed mainly to the strengthening of carbon isotope fractionation in plants caused by increasing precipitation with altitude. Furthermore, there was a remarkable negative correlation between leaf δ¹³C and LNC. One possible reason was that increasing precipitation that operates to increase isotopic discrimination with altitude overtook the LNC in determining the sign of leaf δ¹³C. The significant negative correlation between leaf δ¹³C and SD over altitudes was also found in the present study, indicating that increases in SD with altitude would reduce, rather than enhance plant δ¹³C values.     

Anthropogenic,detritic and atmospheric soil-derived sources of lead in an alpine poor fen in northeast China

Ombrotrophic bogs are faithful archive of atmospheric metal deposition,but the potential for fens to reconstruct environmental change is often underestimated.In this study,some new data on the Pb depositional history in northeast China were provided using two 210Pb-dated peat sequences from a poor fen in the Fenghuang Mountain of Heilongjiang province.Anthropogenic,detritic and atmospheric soil sources were discriminated using a two-step sequential digestion(weak acid leaching to liberate mobile Pb which is often regarded as anthropogenic Pb,especially for recent samples)and a ratio of unsupported 210 Pb and supported 210 Pb with the logic of that the 214 Pb mainly represents the residual detritus(constant throughout the core)and the unsupported 210 Pb arises from atmospheric fallout.A higher 210Pb/214 Pb suggests more contributions from atmospheric deposition to the Pb content in the peat,and a ratio of 10 was defined to indicate the boundary between detritic input and atmospheric deposition.The detritic Pbwas estimated to be 10~(-1)3 mg·kg~(-1),the anthropogenic Pb ranged from 10-80 mg·kg~(-1),and the atmospheric soil-derived Pb ranged from 5 mg·kg~(-1) to 30mg·kg~(-1).The history of anthropogenic Pb pollution over the last 150 years was reconstructed,and the calculated Pb deposition rate(AR Pb) ranged from 5to 56 mg·m-2·yr~(-1).Using Ti as a reliable reference,the enrichment factor of Pb(EF Pb) relative to the upper continental crust was calculated.Both AR Pb and EF Pb increased with time,especially after the foundation of the People's Republic of China.This is consistent with increasing industrialization and coal burning in the last 60 years in northeast China.The present record of anthropogenic Pb deposition was consistent with the previous reports and an increasing trend of environmental pollution due to anthropogenic activities,in contrasts to Europe and North America which have experienced a major environmental cleanup.For the first time,this work estimates atmospheric Pb deposition via a minerotrophic peat core in China.This will enhance the use of peat archives for studies of environmental change.