壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。     

宁夏香山-天景山弧形断裂带新活动特征及1709年中卫南71/2级地震形变带

本文根据野外地质填图和水系位移测量结果,论述了香山-天景山弧形断裂带新生代有两个不同活动性质的阶段,即早期阶段的强烈挤压和晚期阶段的左旋走滑兼挤压。分析、讨论了不同活动阶段的时间界限和转变原因。指出了1709年中卫南71/_2级地震形变带的表现形式、延伸范围     

Static Analysis of Collision Strength of Offshore Platform

In this paper a numerical analysis method combining FEM incemental technique with limit analysis concept is proposed for the study of the static strength of offshore platform in collision. Large deformation and plasticity are accounted for and the limit yield surface expressed by generalized stress for a tubular section is derived. The modified stiffness matrix of space beam element is formulated by Plastic Node Method. The buckling behavior of beam columns can also be taken into account. It can trace the generation of plastic hinges during loading and finally the ultimate strength of offshore platform against collision is obtained.     

地下水资源遥感评估技术研究进展

地下水的赋存和埋深是地下水资源勘察的重要内容。遥感技术具有数据获取快、综合成本低、观测尺度大等诸多优势。基于遥感的地下水资源评估技术一直受到研究人员的关注,也是遥感应用研究中的热点和难点。回顾总结了遥感技术在评估地下水赋存和埋深领域的应用与研究进展,根据不同评估技术的特点将其划分为单因子模型评估法、多因子综合模型评估法、重力卫星数据评估法3种。得出以下结论: ①地下水遥感评估技术经过多年发展,模型方法更加多样,精确度不断提高,可以作为传统地下水资源勘察的重要辅助手段; ②遥感评估地下水赋存的研究发展迅速,但针对地下水埋深信息的评估研究进展相对缓慢; ③高时空分辨率遥感技术和机器学习技术的结合运用、无人机遥感技术的应用是地下水资源遥感评估技术的未来发展方向。     

青海玉树7.1级大震的预测讨论

讨论了2010年4月14日青海玉树大震的震源组合模式。用临界慢化的观点讨论了玉树大震的前震。以异年倍九律与磁暴二倍法相配讨论了玉树大震的发生日期。     

基于GIS的"三旧"改造项目动态监控和管理

"三旧"改造是广东省特有的改造模式,也是未来城市可持续发展战略的重要组成部分.为了全面掌握"三旧"改造用地的工作情况,实现对"三旧"改造项目有效地动态监控,项目采用地理信息系统(GIS)、三维可视化、倾斜摄影、云计算等先进技术,构建了"三旧"改造数据库,搭建了"三旧"改造信息管理平台,并阐述平台实现的相关关键技术.实践表明,平台在改造项目批后、改造项目建设进度等方面起到良好的监控和管理作用,实现了"三旧"改造的精细化管理.     

无限长土坡滑移变形模拟

从建立土体应力平衡微分方程入手,在平面应变的假定下求解均质无限长土坡的应力平衡方程。通过拟定坡土的滑移速度和粘塑性应变率的关系式,以剑桥模型为依托,得出滑移变形速率的显式表达式,由此推出土坡滑动位移的计算式。因为模型计算值与实测值无论变形趋势还是大小都有不少误差,所以经修正粘滞性参数来改进模型的模拟精度。最后通过一个算例,将现场实测滑移数据和模型计算值进行比较,分析表明二者较为一致。     

Automatic mapping of lunar landforms using DEM-derived geomorphometric parameters

Developing approaches to automate the analysis of the massive amounts of data sent back from the Moon will generate significant benefits for the field of lunar geomorphology. In this paper, we outline an automated method for mapping lunar landforms that is based on digital terrain analysis. An iterative self-organizing (ISO) cluster unsupervised classification enables the automatic mapping of landforms via a series of input raster bands that utilize six geomorphometric parameters. These parameters divide landforms into a number of spatially extended, topographically homogeneous segments that exhibit similar terrain attributes and neighborhood properties. To illustrate the applicability of our approach, we apply it to three representative test sites on the Moon, automatically presenting our results as a thematic landform map. We also quantitatively evaluated this approach using a series of confusion matrices, achieving overall accuracies as high as 83.34% and Kappa coefficients (K) as high as 0.77. An immediate version of our algorithm can also be applied for automatically mapping large-scale lunar landforms and for the quantitative comparison of lunar surface morphologies.     

Building national eco-cultural protection areas and the Xiangxi practice in Hunan Province

Journal of Geographical Sciences - Since 2007, the Chinese government has initiated the building of national eco-cultural protection areas (NECPAs), thereby embarking on a significant...